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Method capable of improving tobacco bacterial wilt resistance

A tobacco bacterial wilt and resistance technology, which is applied in the field of using arbuscular mycorrhizal fungi to improve tobacco bacterial wilt resistance, can solve problems such as ecological environment, tobacco plant pollution, affecting tobacco yield and quality, and tobacco plant death. Achieve the effects of improving drought resistance, improving mineral nutrition status, and reducing disease index

Inactive Publication Date: 2015-05-27
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In severely diseased tobacco fields in dry land, the incidence of bacterial wilt can reach 30%-80%, causing large areas of tobacco plants to die or even fail to harvest, seriously affecting the yield and quality of tobacco, and bringing huge economic losses
[0003] At present, the prevention and treatment of bacterial wilt in tobacco production mainly uses microbial preparations prepared by various antagonistic bacteria, such as a preparation method of Bacillus subtilis bacterial agent against tobacco bacterial wilt (CN201110195718.7), which is used to prevent and control tobacco bacterial wilt in continuous cropping Antagonist NJL-14 (CN201010222337.9) or some plant components, such as a botanical fungicide (CN201110317994.6) for preventing and treating tobacco bacterial wilt, or pesticides, such as bromothalonil·nonylcopper compound preparation and its The application of preventing and treating tobacco bacterial wilt (CN201110410302.2), these methods have introduced a large amount of exogenous substances or organisms in use, causing pollution to the ecological environment and tobacco plants, how to safely and effectively improve the resistance of tobacco bacterial wilt still to be resolved

Method used

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  • Method capable of improving tobacco bacterial wilt resistance
  • Method capable of improving tobacco bacterial wilt resistance
  • Method capable of improving tobacco bacterial wilt resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Propagate arbuscular mycorrhizal fungi to prepare a mixed arbuscular mycorrhizal fungal agent:

[0024] The initial species of arbuscular mycorrhizal fungi are Acaulospora mellea, Glomus mosseae, Glomus intraradices; clover or corn as host plant; 3:1 Pass the river sand and soil through a 1mm sieve, sterilize with high pressure steam at 121oC for 2 hours, add 10g of urea and 40g of superphosphate to every 100kg of river sand to form a substrate, and insert 5% of the initial strain of the arbuscular mycorrhizal fungus into the substrate , mix well with the layer method or mixed method, then add 22% water to the substrate, sow the seeds of the host plant, routine management, after 3 months, you can harvest the arbuscular mycorrhizal fungus after the expansion, the method of harvest is Cut off the aerial part of the host plant, cut the root section and mix it evenly, and the air-dried substrate containing the root section of the host plant, the spores of the arbuscular...

Embodiment 2

[0035] (1) Propagate arbuscular mycorrhizal fungi and prepare a mixed arbuscular mycorrhizal fungal agent: the initial strains of arbuscular mycorrhizal fungi are Acaulospora mellea, Glomus mosseae, root Glomus intraradices; clover or corn as the host plant; 3:1 river sand and soil passed through a 1mm sieve, sterilized by dry heat at 160oC for 2 hours, adding 5g of urea and 20g of superphosphate for every 100kg of river sand Form a matrix, insert 3% of the initial bacterial classification of the arbuscular mycorrhizal fungus in the matrix, mix well with layer application or mixed application, then add 18% water in the matrix, sow the seeds of host plants, routine management, After 4 months, the expanded arbuscular mycorrhizal fungi can be harvested. The method of harvesting is to cut off the aboveground part of the host plant, cut the root section and mix it evenly, containing the host plant root section, arbuscular mycorrhizal fungus spores, The air-dried substrate of the my...

Embodiment 3

[0042] (1) Propagate arbuscular mycorrhizal fungi and prepare a mixed arbuscular mycorrhizal fungal agent: the initial strains of arbuscular mycorrhizal fungi are Acaulospora mellea, Glomus mosseae, root Glomus intraradices; clover or corn as host plant; 3:1 river sand and soil passed through a 1mm sieve, sterilized by dry heat at 170oC for 2 hours, and then added with 8g urea and 30g superphosphate for every 100kg river sand Form a matrix, insert 4% of the initial bacterial classification of the arbuscular mycorrhizal fungus in the matrix, mix well with layer application or mixed application, then add 20% water in the matrix, sow the seeds of the host plant, routine management, After 3.5 months, the expanded arbuscular mycorrhizal fungus can be harvested. The method of harvesting is to cut off the aboveground part of the host plant, cut the root section and mix it evenly, containing the host plant root section, arbuscular mycorrhizal fungus spores, The air-dried substrate of ...

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Abstract

The invention provides a method capable of improving tobacco bacterial wilt resistance. The method includes that an initial culture of each arbuscular mycorrhizal fungi is independently propagated, the arbuscular mycorrhizal fungi after mixed and propagated prepares mixed arbuscular mycorrhizal fungicide to infect tobacco; and the initial culture which is propagated by the arbuscular mycorrhizal fungi comprises acaulosporamellea, glomusmosseae and glomusintraradices, and mixing ratio of the acaulosporamellea to the glomusmosseae to the glomusintraradices which are propagated is 0.5:1.1:1 by weight ratio. The method promotes growth of the tobacco, effectively reduces morbidity and disease index of bacterial wilt of the tobacco, and improves resist of the tobacco on the bacterial wilt.

Description

technical field [0001] The invention relates to a method for improving tobacco bacterial wilt resistance, in particular to a method for improving tobacco bacterial wilt resistance by using arbuscular mycorrhizal fungi. Background technique [0002] Bacteria wilt is one of the most common diseases in tobacco growing, which is a bacterial soil-borne disease caused by Ral. solanaceae. In recent years, the disease has generally occurred in Yunnan, Chongqing, Hunan, Fujian and other tobacco-smoking areas south of the Yangtze River. In severely diseased tobacco fields in dry land, the incidence of bacterial wilt can reach 30%-80%, causing large areas of tobacco plants to die or even fail to harvest, seriously affecting the yield and quality of tobacco, and bringing huge economic losses. [0003] At present, the prevention and treatment of bacterial wilt in tobacco production mainly uses microbial preparations prepared by various antagonistic bacteria, such as a preparation method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01G7/06A01G1/00
Inventor 郭涛习向银
Owner SOUTHWEST UNIV
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