Culture medium and application thereof

A culture medium and optional technology are applied in the field of culture medium, preparation of ameloblasts, kits for preparation of ameloblasts, preparation of epithelioid cells, and can solve the problems that preparation methods need to be improved, etc.
CN103205394AActive Publication Date: 2013-07-17GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Publication Date
2013-07-17

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Abstract

The invention discloses a culture medium and application thereof. The culture medium is a DMEM / F12 culture medium containing N2 supplement, retinoic acid and BMP-4. With the culture medium provided by the invention, stem cells can be effectively induced into epithelioid cells; then, the obtained epithelioid cells and mesenchyme of animal dental germs are subjected to recombination, culture and transplantation, so adamantoblast in a dental sample structure can be effectively obtained.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a culture medium and its application, more specifically, to a culture medium, a method for preparing epithelioid cells, a kit for preparing ameloblasts and a method for preparing ameloblasts. Background technique

[0002] At present, inducing stem cells into specific tissue cell types and using them in the treatment of corresponding tissue lesions is a new way to treat many diseases at this stage. In recent years, researchers in the field of regenerative medicine and tissue engineering have mainly used stem cells as seed cells to induce the preparation of specific tissue cell types, thereby providing a large number of materials for clinical transplantation of cells, tissues or organs, and then used in Diabetes, Parkinson's disease, spinal cord injury, leukemia, myocardial injury, renal failure, liver cirrhosis and other diseases, and great progress has been made so far.

[0003] Howev...

Claims

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