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A kind of high performance liquid chromatography detection method of penicillin g in penicillin scum

A technology of high-performance liquid chromatography and penicillin residue, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that penicillin G cannot be detected quickly and accurately, and achieve the effect of high accuracy, high sensitivity and good linear relationship

Active Publication Date: 2015-08-19
湖南哈工机器人研究院有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] The purpose of the present invention will solve the problem that penicillin G in the penicillin slag cannot be detected quickly and accurately in the prior art, and a high performance liquid chromatography detection method for penicillin G in the penicillin slag is provided

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  • A kind of high performance liquid chromatography detection method of penicillin g in penicillin scum
  • A kind of high performance liquid chromatography detection method of penicillin g in penicillin scum

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specific Embodiment approach 1

[0015] Specific embodiment one: present embodiment is a kind of high performance liquid chromatography detection method of penicillin G in penicillin scum, specifically completes according to the following steps:

[0016] 1. Draw the standard curve and fit the standard curve equation: the penicillin G standard is dissolved in a solvent, and diluted with a solvent to prepare a concentration of 0.1 μg·mL -1 ~2000μg·mL -1 Penicillin G standard solutions with different gradient concentrations between them were filtered through a 0.45 μm needle filter and detected by high performance liquid chromatography. Chromatography detection is drawn into standard curve, then fits, and obtains standard curve equation: Y=kX+b, Y represents the chromatographic peak area in the equation, and X represents the concentration of penicillin G, and k and b are constants; Described solvent is by Acetonitrile and water are mixed according to the volume ratio of acetonitrile and water is 1:1;

[0017] ...

specific Embodiment approach 2

[0026] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the high performance liquid chromatography method described in step one concrete operation is as follows: HPLC that forms with binary pump, constant temperature box, ultraviolet detector and chemical workstation instrument, with the mass fraction of 0.3% formic acid aqueous solution / acetonitrile as the mobile phase, the volume ratio of aqueous phase A and organic phase B in the present invention is 60:40 with a flow rate of 1.0mL·min -1 Constant elution. Constant elution, chromatographic conditions are as follows:

[0027] a. Column: Eelipse XDB-C 18 ;

[0028] B, mobile phase: water phase A: mass fraction is the formic acid aqueous solution of 0.3%; Organic phase B: acetonitrile; And the volume ratio of described water phase A and organic phase B is 60:40;

[0029] c. Elution method: isocratic elution 0.8mL min -1 ~1.2mL·min -1 ;

[0030] d. Detection wavelength: UV215...

specific Embodiment approach 3

[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: in step 2, at first penicillin bacterium residue is added in the extraction liquid, after mixing evenly, ultrasonically extracts 30min, then carries out centrifugation, centrifugation obtains The supernatant was the penicillin G crude extract. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a high performance liquid chromatography detection method for penicillin G in penicillin fungi residues, which relates to a detection method for penicillin G in penicillin fungi residues, and aims to solve the problems in the prior art that penicillin G in penicillin fungi residues cannot be rapidly and accurately detected. The method comprises the following steps of: 1, drawing a standard curve and fitting a standard curvilinear equation; 2, extracting to obtain an extraction solution of the penicillin G; 3, carrying out activation treatment; 4, eluting to obtain a solution to be detected; and 5, determining the content of the penicillin G. The high performance liquid chromatography detection method disclosed by the invention is mainly used for detecting the content of the penicillin G in the penicillin fungi residues.

Description

technical field [0001] The invention relates to a method for detecting penicillin G in penicillin scum. Background technique [0002] Antibiotics, as a major representative veterinary drug, have received widespread attention in today's rapid development of food safety and world trade. At present, their detection is still mainly concentrated in animal-derived foods. The main detection methods are high-performance liquid chromatography. method, high performance liquid chromatography-tandem mass spectrometry, capillary electrophoresis, enzyme-linked immunoassay. [0003] Antibiotic fungus residue is the waste of antibiotic production enterprises in the production process. The substrate is mainly composed of soybeans, peanut cakes, corn protein, starch, etc. The appearance is like bean curd residue, and the smell varies with different cultured fungi; the inside contains coarse Nutrients such as protein, fat, sugar, water and a small amount of antibiotic residues will become sme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/14
Inventor 刘惠玲王璞
Owner 湖南哈工机器人研究院有限公司
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