Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High performance liquid chromatography detection method for penicillin G in penicillin fungi residues

A high performance liquid chromatography, penicillin bacteria residue technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of inability to detect penicillin G quickly and accurately, and achieve high accuracy, improve detection efficiency, and good linear relationship. Effect

Active Publication Date: 2013-10-02
湖南哈工机器人研究院有限公司
View PDF5 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to solve the problem that the penicillin G in the penicillin slag cannot be quickly and accurately detected in the prior art, and to provide a high-efficiency method for penicillin G in the penicillin slag. Liquid chromatography detection method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High performance liquid chromatography detection method for penicillin G in penicillin fungi residues
  • High performance liquid chromatography detection method for penicillin G in penicillin fungi residues

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0016] Specific embodiment one: present embodiment is a kind of high performance liquid chromatography detection method of penicillin G in penicillin scum, specifically completes according to the following steps:

[0017] 1. Draw the standard curve and fit the standard curve equation: the penicillin G standard is dissolved in a solvent, and diluted with a solvent to prepare a concentration of 0.1 μg·mL -1 ~2000μg·mL -1 Penicillin G standard solutions with different gradient concentrations between them were filtered through a 0.45 μm needle filter and detected by high performance liquid chromatography. Chromatography detection is drawn into standard curve, then fits, and obtains standard curve equation: Y=kX+b, Y represents the chromatographic peak area in the equation, and X represents the concentration of penicillin G, and k and b are constants; Described solvent is by Acetonitrile and water are mixed according to the volume ratio of acetonitrile and water is 1:1;

[0018] ...

specific Embodiment approach 2

[0027] Embodiment 2: The difference between this embodiment and Embodiment 1 is: the specific operation of the high-performance liquid chromatography method described in step 1 is as follows: an HPLC composed of a binary pump, a thermostat, an ultraviolet detector and a chemical workstation In the present invention, the volume ratio of aqueous phase A and organic phase B is 60:40, and the flow rate is 1.0 mL min. -1 Constant elution. Constant elution, chromatographic conditions are as follows:

[0028] a. Chromatographic column: Eelipse XDB-C 18 ;

[0029] B, mobile phase: water phase A: the formic acid aqueous solution whose mass fraction is 0.3%; organic phase B: acetonitrile; and the volume ratio of the water phase A and the organic phase B is 60:40;

[0030] c. Elution method: isocratic elution 0.8mL·min -1 ~1.2mL·min -1 ;

[0031] d. Detection wavelength: UV215nm;

[0032] e. Column temperature: 30℃;

[0033] f. Injection volume: 10 μL.

[0034] Others are the sa...

specific Embodiment approach 3

[0038] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that: in step 2, the penicillin bacterial residue is firstly added to the extraction solution, and after mixing evenly, ultrasonic extraction is performed for 30 minutes, and then centrifugation is performed. The supernatant is the crude extract of penicillin G. Others are the same as in the first or second embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a high performance liquid chromatography detection method for penicillin G in penicillin fungi residues, which relates to a detection method for penicillin G in penicillin fungi residues, and aims to solve the problems in the prior art that penicillin G in penicillin fungi residues cannot be rapidly and accurately detected. The method comprises the following steps of: 1, drawing a standard curve and fitting a standard curvilinear equation; 2, extracting to obtain an extraction solution of the penicillin G; 3, carrying out activation treatment; 4, eluting to obtain a solution to be detected; and 5, determining the content of the penicillin G. The high performance liquid chromatography detection method disclosed by the invention is mainly used for detecting the content of the penicillin G in the penicillin fungi residues.

Description

technical field [0001] The invention relates to a method for detecting penicillin G in penicillin scum. Background technique [0002] Antibiotics, as a major representative veterinary drug, have received widespread attention in today's rapid development of food safety and world trade. At present, their detection is still mainly concentrated in animal-derived foods. The main detection methods are high-performance liquid chromatography. method, high performance liquid chromatography-tandem mass spectrometry, capillary electrophoresis, enzyme-linked immunoassay. [0003] Antibiotic fungus residue is the waste of antibiotic production enterprises in the production process. The substrate is mainly composed of soybeans, peanut cakes, corn protein, starch, etc. The appearance is like bean curd residue, and the smell varies with different cultured fungi; the inside contains coarse Nutrients such as protein, fat, sugar, water and a small amount of antibiotic residues will become sme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88G01N30/14
Inventor 刘惠玲王璞
Owner 湖南哈工机器人研究院有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com