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Hydrophilic and hydrophobic patterned array chip, preparation method and application thereof

An array chip and patterning technology, applied in the field of mass spectrometry detection, can solve the problems of increased cost and increased application difficulty

Active Publication Date: 2022-04-22
中科新芯纳米技术(常州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, by visually inspecting changes in the turbidity of liquids, this requires a certain amount of practice to judge correctly, increasing the difficulty of application
In addition, if you want to realize automatic operation, you need to configure the corresponding shaking equipment, such as: vibrator, which will increase the cost virtually.

Method used

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  • Hydrophilic and hydrophobic patterned array chip, preparation method and application thereof
  • Hydrophilic and hydrophobic patterned array chip, preparation method and application thereof
  • Hydrophilic and hydrophobic patterned array chip, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Put the monocrystalline silicon [n type, (100)] into acetone, chloroform, ethanol, and ultrapure water for ultrasonic cleaning respectively, and blow dry with high-purity nitrogen. Then use O 2 -Plasma is treated with 100W power for 2 minutes to hydroxylate the surface of the silicon wafer.

[0040] Paste the hydroxylated silicon wafer on a clean upper surface dish, pipette 10 μL of heptadecafluorodecyltrimethoxysilane dropwise onto the lower surface dish, then fasten the upper surface dish, and heat and grow at 150°C for 2 hours , so that the surface of the entire silicon wafer is modified with fluorine-containing silane, making the surface of the silicon wafer hydrophobic.

[0041] The evaporation mask with arrayed pattern (the pattern can be: circle, triangle, square or polygon, etc., the size of the pattern in the mask is 100-2000 μm, and the distance between the patterns is 500-5000 μm) is covered on the modified surface containing Fluorine monolayer silicon wafe...

Embodiment 2

[0044] The glass slides were ultrasonically cleaned in acetone, chloroform, ethanol, and ultrapure water, and dried with high-purity nitrogen. Then use O 2 -Plasma is treated with 100W power for 2 minutes to hydroxylate the surface of the glass sheet.

[0045] Attach the hydroxylated glass slide to a clean upper surface dish, pipette 10 μL of heptadecylfluorodecyltrimethoxysilane to the lower surface dish dropwise, then fasten the upper surface dish, and heat and grow at 120°C for 3 hours , so that the surface of the entire glass sheet is modified with fluorine-containing silane, making the surface of the glass sheet hydrophobic.

[0046] The evaporation mask with arrayed pattern (the pattern can be: circle, triangle, square or polygon, etc., the size of the pattern in the mask is 100-2000 μm, and the distance between the patterns is 500-5000 μm) is covered on the modified surface containing Fluorine monolayer glass slide, and put the substrate covered with the baffle upside...

Embodiment 3

[0049] Put the quartz slices into acetone, chloroform, ethanol, and ultrapure water, respectively, to ultrasonically clean them, and dry them with high-purity nitrogen. Then use O 2 -Plasma is treated with 100W power for 2 minutes to hydroxylate the surface of the quartz plate.

[0050] Paste the hydroxylated quartz plate on a clean upper surface dish, pipette 20 μL of tridecafluorooctyltrimethoxysilane dropwise on the lower surface dish, then fasten the upper surface dish, and heat and grow at 100°C for 5 hours , so that the entire surface is modified with fluorine-containing silane, making the surface hydrophobic.

[0051] The evaporation mask with arrayed pattern (the pattern can be: circle, triangle, square or polygon, etc., the size of the pattern in the mask is 100-2000 μm, and the distance between the patterns is 500-5000 μm) is covered on the modified surface containing Fluorine monolayer quartz wafer, and put the substrate covered with the baffle upside down into th...

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Abstract

The invention belongs to the field of mass spectrometry detection, and particularly relates to a hydrophilic and hydrophobic patterned array chip which can be widely applied to preparation and detection of a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF MS) sample. The method comprises the following steps: constructing an array chip of which the outer ring is a hydrophobic layer and the middle is a hydrophilic point on a substrate; the middle hydrophilic point is a metal film or a nanoparticle assembled film; and dropwise adding a mixed solution of the matrix and the biological sample onto the hydrophilic area of the chip, and naturally airing at room temperature to obtain uniform and fine co-crystallized particles of the matrix and the sample. By utilizing the chip disclosed by the invention, not only can the enrichment of a biological sample be realized, but also the co-crystallization morphology of a matrix and the sample can be improved, so that the sensitivity and the reproducibility of MALDI-TOF MS detection are improved, and the chip has a great practical application value.

Description

technical field [0001] The invention belongs to the field of mass spectrometry detection, and in particular relates to a preparation method of a hydrophilic-hydrophobic patterned array chip and an application in the field of mass spectrometry detection. Background technique [0002] Precision medicine is an emerging medical method that applies modern genetic technology, molecular imaging technology, and bioinformatics technology, combined with patients' life and clinical data, to achieve accurate disease classification and diagnosis, and to formulate personalized disease prevention and treatment plans. However, to fully realize precision medicine, precise diagnosis and precise treatment are inseparable. Among them, advanced clinical detection technology is the prerequisite for accurate diagnosis. [0003] As an emerging technology with high specificity, high sensitivity, high accuracy, fast analysis speed, and simultaneous detection of multiple indicators, mass spectrometry...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N27/64
CPCB01L3/502707G01N27/64Y02A50/30
Inventor 曾周芳王燕东
Owner 中科新芯纳米技术(常州)有限公司
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