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Method for detecting efficacy of classical swine fever live vaccines

A swine fever live vaccine and testing method technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of complex strains, time-consuming, and difficulty in accurately testing the efficacy of live vaccines, and achieve uniform conditions, low cost, and experimental results. significantly different effects

Active Publication Date: 2013-10-02
PU LIKE BIO ENG
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Problems solved by technology

Not only does it require a lot of experiments with white rabbits, which is time-consuming and laborious, but also because the number of pure white rabbits in my country is gradually decreasing and the strains are becoming more and more complex, it is difficult to accurately detect the efficacy of live vaccines by using rabbit body reaction heat

Method used

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  • Method for detecting efficacy of classical swine fever live vaccines
  • Method for detecting efficacy of classical swine fever live vaccines

Examples

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Embodiment 1

[0031] Test method for efficacy of swine fever live vaccine

[0032] 1. Preparation of Cells for Passaging

[0033] Pig testicular (ST) cells (derived from ATCC, American Type Culture Collection) covered with a single layer were digested and dispersed with 0.25% trypsin (containing 0.02% EDTA) and then added with 3% fetal bovine serum α-MEM cells were maintained in culture medium, and after cell counting, the cell suspension was added to a 96-well cell plate, 100 μl per well.

[0034] 2. Dilution of live swine fever vaccine

[0035] In this example, three batches of live vaccines were prepared, and their antigen contents were determined respectively.

[0036] The strain of the live vaccine of swine fever is the attenuated strain of swine fever lathe (preserved by China Veterinary Drug Administration, preservation number AV1412). Inoculate ST cells with attenuated CSF rabbitized strain, and mix the harvested virus antigen solution with appropriate lyoprotectant to make CSF l...

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Abstract

The invention belongs to the technical field of novel biological medicines of veterinary medicines, and in particular relates to a method for detecting the efficacy of classical swine fever live vaccines. According to the method, the efficacy of live vaccines can be evaluated by detecting the virus content of live vaccines by an indirect immunofluorescence (IFA) method. The method is short in detection time, simple to operate, low in cost, accurate in result and good in repeatability, and has a good application value and wide market prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for testing the efficacy of a swine fever live vaccine. Background technique [0002] Classical Swine fever (CSF for short), also known as Hog cholera or European swine fever, is a viral infectious disease with acute or hyperacute fever in pigs, clinically characterized by dissemination Rapid, febrile, typical hemorrhagic lesions can be seen on autopsy. Swine fever can infect pigs of all ages and is prevalent throughout the year with high morbidity and mortality, which is extremely harmful to pigs. This disease is one of the most important infectious diseases threatening the pig industry. The World Organization for Animal Health (OIE) lists swine fever as one of the 16 notifiable infectious diseases of category A, and China defines it as a severe infectious disease. Mandatory immunization with swine fever live vaccines is currently the best way to prevent swine fever in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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