Application of frankincense extract in prevention and treatment of cardiovascular and cerebrovascular or liver and kidney damage

An extract, liver injury technology, applied in the application field of frankincense extract in the prevention and treatment of cardiovascular and cerebrovascular or liver and kidney injury, can solve the problems of reducing drug absorption and bioavailability, limiting clinical application, etc., to improve bioavailability, Good efficacy and fast onset effect

Inactive Publication Date: 2015-10-14
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the active ingredients of frankincense extract are water-insoluble, the commonly used clinical dosage forms of frankincense and its compound are pills, capsules, tablets, powders, ointments and other oral or external preparations, and there are no intravenous dosage forms, which makes drug absorption and bioavailability The degree is greatly reduced, which greatly limits the clinical application of the drug

Method used

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  • Application of frankincense extract in prevention and treatment of cardiovascular and cerebrovascular or liver and kidney damage
  • Application of frankincense extract in prevention and treatment of cardiovascular and cerebrovascular or liver and kidney damage
  • Application of frankincense extract in prevention and treatment of cardiovascular and cerebrovascular or liver and kidney damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 实施例1:乳香醇提物乳剂的制备方法

[0028] 乳香提取物10-30%、乙醇1-5%、卵磷脂10-30%、精致大豆油5-25%、油酸0.5-6.5%、生育酚0.01-1.0%、注射用生理盐水添至100%。

[0029] 取乳香提取物200mg,溶于无水乙醇100μL中,于50℃下高速搅拌使其溶解均匀,加入精致大豆油200mg,油酸80mg,生育酚1mg,于50℃下高速搅拌使其溶解均匀,制成油相;卵磷脂200mg溶于适量注射用生理盐水中,于50℃下高速搅拌使其溶解均匀制成水相;油水两相混合于60℃高速搅拌制成初乳;加之注射用生理盐水至2ml,调节pH至5.0-7.0,至高压乳匀机中,反复乳化至乳滴平均粒径≤0.5μm。取上述乳剂过滤后,充氮气灌装,灭菌既得。

Embodiment 2

[0030] 实施例2:乳香对大鼠心肌缺血 / 再灌注(MI / R)损伤的保护作用

[0031] SD大鼠40只,体重220-280g,购自第四军医大学动物实验中心。动物分笼饲养(环境温度24℃,湿度60-70%),术前6d禁食、自由饮水。10%水合氯醛腹腔注射麻醉,仰卧固定,链接心电图检测,气管插管并接呼吸机(频次:75次 / min ;潮气量:8-9 mL;吸:呼=1:2),乳香乳剂静脉注射(3各剂量水平200,100,50 mg / kg),30 min后眼眶采血1.0mL。给药30 min后,胸骨左缘旁纵行切开,第3-5肋间做横向切口,挑出心脏,以左心耳与肺动脉圆锥交界处的左冠状动脉前降支(Left anterior descending coronary aery, LAD)为标志,于左心耳下方1-2mm处进针,穿线(横跨左冠状动脉前降支),结扎冠状动脉前降支血管30 min后再灌注4h时取血测定CK-MB和LDH活性,取心脏组织测定心肌梗死面积。结果(见表1):高中低三个剂量乳香乳剂均能显著预防缺血 / 再灌注造成的心肌损伤,与模型组相比可明显提高CK-MB和LDH活性,同时减小心肌梗死面积( figure 1 ),表明乳香乳剂具有心肌损伤保护作用。

[0032]

Embodiment 3

[0033] 实施例3:乳香对大鼠脑缺血 / 再灌注(RI / R)损伤的保护作用

[0034] SD大鼠40只,体重220-280g,购自第四军医大学动物实验中心。 动物分笼饲养(环境温度24℃,湿度60-70%),术前6d禁食、自由饮水。 10%水合氯醛腹腔注射麻醉,仰卧位固定,乳香乳剂静脉注射(3各剂量水平200,100,50 mg / kg),30 min后,于颈正中位切口,暴露颈右侧总动脉(CCA )、颈外动脉(ECA)及翼腭动脉,在颈总动脉分叉处下剪纵形切口,将预先用酒精灯将两端烧成圆头的尼龙线(30,美国)置入颈内 动脉内约17-18mm,阻断2h后抽出县栓,再灌注24h时取血测定神经功能性评分(0 分为无功能障碍;1 分为不能伸展左侧前肢;2 分为向左侧旋转 ;3 分为向左侧倾倒;4 分为无自主活动伴意识障碍;分数越高,表示动物神经功能损伤越大)、神经元特异性烯醇化酶(NSE)活性,取脑组织测定脑梗死 area. The result is as Figure 2-4 :高中低三个剂量乳香乳剂均能显著预防缺血 / 再灌注造成的脑损伤,与模型组相比可明显降低神经功能性评分,提高NSE活性,同时减小心肌梗死面积,表明乳香乳剂具有脑损伤保护作用。

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Abstract

The invention discloses application of a frankincense extract in preparation of a drug for treating a cardio-cerebral-vascular disease or liver and kidney injury. An animal in-vivo research result shows that frankincense emulsion for injection has a significant effect of relieving the liver and kidney injury, simultaneously has a certain protection effect on cardiac muscles and cerebral vascular injury. The frankincense emulsion for injection has the characteristics of high bioavailability and good availability and therefore shows important practical value and application prospect.

Description

technical field [0001] The invention relates to frankincense extract and its application in preventing and treating cardiovascular and cerebrovascular or liver and kidney damage. Background technique [0002] The heart, brain, liver and kidney are important organs of the human body, and related diseases seriously threaten human health. Coronary heart disease is one of the diseases with the highest mortality rate in the world, and the number of deaths in China ranks second in the world; stroke, the annual death toll in the world is about 6 million, and the incidence rate in China is the first in the world; kidney injury disease, the incidence rate in developed countries is 6.5% -10%, while the incidence rate in some areas of China is as high as 8%-12%. Since 2005, there have been about 60,000 uremia patients receiving renal replacement therapy, and the annual growth rate is greater than 11%. It has become a common clinical critical disease diseases, among which the fatality ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/324A61P1/16A61P31/14A61P31/20A61P35/00A61P11/00A61P13/12A61P9/10A61P25/08A61P25/16A61P25/28A61P9/06A61P9/04A61P9/12A61P31/06A61P3/10A61P13/04A61P25/02A61P9/00
CPCY02A50/30
Inventor 文爱东赵瑾怡刘美佑陈敏纯贾艳艳王珊孙渊朱晓鹤
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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