Application of fluevirosines A in the preparation of drugs for inhibiting liver fibroblast proliferation

A technology of fibroblasts and drugs, which can be used in drug combinations, pharmaceutical formulations, organic active ingredients, etc., and can solve problems such as lack of clinical treatment methods

Inactive Publication Date: 2015-08-05
SUZHOU CHENCAI TEXTILE RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some anti-hepatic fibrosis treatments have appeared successively, including chemical drugs, biological agents, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking. The main links are: reduce liver damage; inhibit the activation of stellate cells, reduce the production of extracellular matrix; regulate the disorder of cytokines, promote the apoptosis of activated hepatic stellate cells

Method used

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  • Application of fluevirosines A in the preparation of drugs for inhibiting liver fibroblast proliferation
  • Application of fluevirosines A in the preparation of drugs for inhibiting liver fibroblast proliferation
  • Application of fluevirosines A in the preparation of drugs for inhibiting liver fibroblast proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: the preparation of the compound Fluevirosines A tablet involved in the present invention:

[0011] Take 5 grams of compound Fluevirosines A, add 195 grams of dextrin, mix well, and make 1000 tablets by conventional compression.

Embodiment 2

[0012] Embodiment 2: the preparation of compound Fluevirosines A capsules involved in the present invention:

[0013] Get 5 grams of compound Fluevirosines A, add 195 grams of starch, mix well, and pack into capsules to make 1000 capsules.

[0014] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example 1

[0015] Experimental example 1: MTT method was used to evaluate the inhibitory effect of the compound Fluevirosines A on liver fibrosis

[0016] 1. Method: NIH / 3T3 cell line was purchased from the cell bank of Shanghai Institute of Cellular Sciences, Chinese Academy of Sciences, cited from ATCC (American Type Culture Collection). Subconfluent NIH / 3T3 cells in the logarithmic growth phase were digested with 0.25% trypsin, washed, centrifuged, and made 1×10 with DMEM medium (containing 10% FCS). 4 cell / ml cell suspension, the survival rate identified by trypan blue staining is greater than 95%, add 100ul per well to a 96-well plate, and store at 37°C, 5% CO 2 After 24 hours of culture and synchronous treatment, the supernatant was discarded, and 200ul of DMEM culture solution (containing 10% FCS) containing different dilutions of drugs was added, cultured for 48 hours, and MTT solution was added to each well for incubation for 4 hours. Discard the culture medium, add 150ulDMSO, ...

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Abstract

The invention discloses the application of fluevirosines A in preparation of the medicine inhibiting liver fibroblast proliferation. The pharmacological test shows that the fluevirosines A can inhibit generation and development of liver fibrosis obviously and can be used for preparing the medicine for preventing liver fibrosis and preventing and curing liver cirrhosis. The fluevirosines A belongs to a brand new matrix type, has the advantages of being high in inhibitory activity to liver fibroblast proliferation and prominent in substantive features, and makes remarkable progress in prevention of liver fibroblast proliferation.

Description

technical field [0001] The invention relates to a new application of the compound Fluevirosines A, in particular to the application of the Fluevirosines A in the preparation of drugs for inhibiting the proliferation of liver fibroblasts. Background technique [0002] Liver fibrosis is a dynamic process from chronic liver injury to liver cirrhosis. In the past 20 years, the research on liver fibrosis has made great progress, and it has been confirmed that liver fibrosis and liver cirrhosis are reversible to a certain extent. In recent years, some anti-hepatic fibrosis treatments have appeared successively, including chemical drugs, biological agents, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking. The main steps are: reduce liver damage; inhibit the activation of stellate cells, reduce the production of extracellular matrix; regulate the disorder of cytokines, and promote the apoptosis of activated hepatic stell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/407A61P1/16
Inventor 袁永春宋巧燕李玉萍
Owner SUZHOU CHENCAI TEXTILE RES & DEV
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