High-throughput screening method for bacterial strains capable of generating quorum sensing signal molecules

Abstract

A high-throughput screening method for bacterial strains capable of generating quorum sensing signal molecules comprises the following steps: step 1, preparing a reporter plate which is a solid microbiological medium with distributed reporter strains; step 2, separating to-be measured strain to obtain single colonies of the to-be measured strain; step 3, transferring the to-be measured strain: photocopying the single colonies of the to-be measured strain to the reporter plate described in the step 1 by using a film covering method; and step 4, obtaining the strain capable of expressing the signal molecules: culturing the reporter plate described in the step 3 until the expression signal is detectable, wherein the bacterial strain, which is in the single colonies of the to-be measured strain and is corresponding to the position of displaying the expression signal in the reporter plate, is the bacterial strain capable of expressing the signal molecules. The method is capable of screening 40-60 single colonies in one time and further improving screening efficiency when the screening platform is enlarged, and the screening obtained results are reliable, and the generation rates of false positive and false negative are low.

Description

technical field [0001] The invention relates to a strain screening method, relates to the field of biotechnology, in particular to a high-throughput screening method for producing quorum sensing signal molecule strains. Background technique [0002] Quorum sensing (quorum sensing, QS) phenomenon refers to a physiological behavior in which bacterial cells regulate gene expression according to density changes, regulating the expression of many characteristics related to food spoilage, animal and plant pathogenicity, such as protease activity, siderophilicity, etc. hormone production, etc. N-acyl-homoserine lactones (AHLs) are the most typical signaling molecules in the quorum sensing system of Gram-negative bacteria. As a key factor in the bacterial QS system, signaling molecules are the language of conversation between bacteria. Therefore, how to quickly and accurately determine whether bacteria produce signal molecules has become an important means to study the bacterial Q...

AI Technical Summary

Problems solved by technology

This method is used as a conventional method for strain screening, but due to the large number of strains to be screened, a large amount of repetitive work is often required to screen the target strain, so it is necessary to develop a high-throughput method that can save workload. Quantitative screening method

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