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Application of phyllanthoid A in the preparation of drugs for inhibiting liver fibroblast proliferation

A technology of fibroblasts and drugs, applied in drug combinations, pharmaceutical formulations, organic active ingredients, etc., can solve problems such as lack of clinical treatment methods

Active Publication Date: 2016-03-30
上海埃泽思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some anti-hepatic fibrosis treatments have appeared successively, including chemical drugs, biological agents, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking. The main links are: reduce liver damage; inhibit the activation of stellate cells, reduce the production of extracellular matrix; regulate the disorder of cytokines, promote the apoptosis of activated hepatic stellate cells

Method used

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  • Application of phyllanthoid A in the preparation of drugs for inhibiting liver fibroblast proliferation
  • Application of phyllanthoid A in the preparation of drugs for inhibiting liver fibroblast proliferation
  • Application of phyllanthoid A in the preparation of drugs for inhibiting liver fibroblast proliferation

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Effect test

Embodiment 1

[0011] Embodiment 1: the preparation of the compound PhyllanthoidA tablet involved in the present invention:

[0012] Take 5 grams of compound Phyllanthoid A, add 195 grams of dextrin, mix well, and make 1000 tablets by conventional compression.

Embodiment 2

[0013] Embodiment 2: the preparation of compound PhyllanthoidA capsules involved in the present invention:

[0014] Get 5 grams of compound Phyllanthoid A, add 195 grams of starch, mix well, pack into capsules and make 1000 capsules.

[0015] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example 1

[0016] Experimental example 1: MTT method was used to evaluate the inhibitory effect of compound Phyllanthoid A on liver fibrosis

[0017] 1. Method: NIH / 3T3 cell line was purchased from the cell bank of Shanghai Institute of Cellular Sciences, Chinese Academy of Sciences cited from ATCC (American Type Culture Collection). Subconfluent NIH / 3T3 cells in the logarithmic growth phase were digested with 0.25% trypsin, washed, centrifuged, and made 1×10 with DMEM medium (containing 10% FCS). 4 cell / ml cell suspension, the survival rate identified by trypan blue staining is greater than 95%, add 100ul per well to a 96-well plate, and store at 37°C, 5% CO 2 After 24 hours of culture and synchronous treatment, the supernatant was discarded, and 200ul of DMEM culture solution (containing 10% FCS) containing different dilutions of drugs was added, cultured for 48 hours, and MTT solution was added to each well for incubation for 4 hours. Discard the culture medium, add 150ulDMSO, shake ...

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Abstract

The invention discloses application of Phyllanthoid A in preparing a medicine for treating liver fibrosis. Pharmacological tests prove that Phyllanthoid A can obviously inhibit the occurrence and development of liver fibrosis and can be used for preparing a medicine for resisting liver fibrosis and preventing liver cirrhosis; the Phyllanthoid A belongs to a brand new framework type; with high inhibitory activity on liver fibroblast proliferation, the Phyllanthoid A has outstanding substantive features; meanwhile, a remarkable progress is obviously made in resisting liver fibroblast proliferation.

Description

technical field [0001] The present invention relates to a new application of compound Phyllanthoid A, in particular to the application of Phyllanthoid A in the preparation of drugs for inhibiting liver fibroblast proliferation. Background technique [0002] Liver fibrosis is a dynamic process from chronic liver injury to liver cirrhosis. In the past 20 years, the research on liver fibrosis has made great progress, and it has been confirmed that liver fibrosis and liver cirrhosis are reversible to a certain extent. In recent years, some anti-hepatic fibrosis treatments have appeared successively, including chemical drugs, biological agents, traditional Chinese medicine and gene therapy, etc., but the ideal clinical treatment methods are still lacking. The main steps are: reduce liver damage; inhibit the activation of stellate cells, reduce the production of extracellular matrix; regulate the disorder of cytokines, and promote the apoptosis of activated hepatic stellate cells....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/58A61P1/16
Inventor 马保凤李一莹李鸣娟付慧玲
Owner 上海埃泽思生物科技有限公司
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