Method for identifying microorganisms by adopting mass spectrum

A microbial and mass spectrometry technology, applied in the field of microbial identification, can solve the problems of increasing false positives and false positives, increasing security incidents, and low accuracy of microbial identification, improving the accuracy, reducing the probability of false positives and false positives, and reducing The effect of false positives and false positives

Active Publication Date: 2014-03-05
融智生物科技(青岛)有限公司
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Problems solved by technology

[0004] However, the above microbial analysis and identification method can only compare the mass numbers of some characteristic peaks of the microorganisms to be tested, and cannot compare the mass numbers of all the characteristic peaks of the microorganisms to be tested, resulting in a low identification accuracy of microorganisms, which is only 80%-95%. Thereby increasing the probability of misjudgment and false alarm, and also increasing the safety accidents caused by microbial misjudgment and false alarm

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  • Method for identifying microorganisms by adopting mass spectrum
  • Method for identifying microorganisms by adopting mass spectrum
  • Method for identifying microorganisms by adopting mass spectrum

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Experimental program
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Embodiment

[0070] Obtain a list of mass spectrum peaks of all known microorganisms (as shown in Table 1);

[0071] Determine the mass intervals of all characteristic peaks in the peak list, and divide the mass intervals into several units, so that the characteristic peaks of each microorganism fall into different units;

[0072] Obtain the peak-out ratio X or the peak-out ratio Y of each known microorganism in each unit;

[0073] Set the upper limit value of all unit weighting values ​​to A, and the lower limit value to negative A,

[0074] Obtain the weighted value of each known microorganism in each unit (as shown in Table 2), specifically calculated according to the following formula:

[0075] A*X or (-A)*Y;

[0076] Obtain the product of the peak intensity of each known microorganism in each unit and the weighted value, and the sum of the weighted products of all units of the microorganism (as shown in Table 3);

[0077] Obtain the mass spectrum peak list of the microorganisms to ...

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Abstract

The embodiment of the invention provides a method for identifying microorganisms by adopting mass spectrum. The method provided by the embodiment of the invention has the advantages that by adoption of a unit partitioning method, the characteristic and the specificity of microorganisms are relatively well reflected; by adoption of a weighting product sum method of the microorganisms, the correlation between the peak intensity and the peak-appearing rule of the microorganisms is relatively well embodied in a full and comprehensive manner; and therefore, all the known microorganisms are used for verifying the method, and the identification accuracy of the microorganisms is more than 99%, so that the accuracy is greatly improved, the probability of the misjudgment and the false alarm is effectively reduced, and safety accidents caused by the misjudgment and the false alarm of the microorganisms are also reduced.

Description

technical field [0001] The invention relates to the field of microorganism identification, in particular to a method for identifying microorganisms by mass spectrometry. Background technique [0002] In related technologies, mass spectrometry methods for identifying microorganisms, including sample pretreatment methods, microbial analysis and identification methods, and reporting methods for identification results, all use mass spectrometry protein detection methods—MALDI-TOF method. [0003] Among them, the microbial analysis and identification method is completed by comparing the list of characteristic peaks of the detected microorganisms with a characteristic spectrum library containing a large amount of microbial characteristic data. A library of signature spectra is prepared by performing several measurements of known microbial species and strains under slightly different conditions. When using mass spectrometry to identify microorganisms, extract information such as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 佟雪梅赵焕铸
Owner 融智生物科技(青岛)有限公司
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