Method for detecting drug resistance of rhopalosiphum aphids

The invention relates to a technology for constricting aphid and drug resistance, which is applied in the field of monitoring the drug resistance of agricultural pests, and can solve the problems of shortening the time for inspection results, complicated operation, and no improvement, and achieves convenient and reliable rapid detection and solves the problems of using insects. Large volume and clear results

Active Publication Date: 2014-03-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Toxicity determination is a common method for indoor understanding of insecticide resistance, but the traditional immersion method and drip method are cumbersome to perform, and several concentrations must be set and repeated to ensure the reliability of the results.
The glass tube drug film method is a new method for rapid detection of pest resistance. Due to its obvious effect, it shortens the time for checking results, but it is still the same as the traditional method in terms of concentration and repetition.

Method used

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  • Method for detecting drug resistance of rhopalosiphum aphids
  • Method for detecting drug resistance of rhopalosiphum aphids
  • Method for detecting drug resistance of rhopalosiphum aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Obtaining the diagnostic dose

[0025] 1. Research object

[0026] The research object of the present invention is a mixed population of Rhopalosiphum padi from various places that have been raised in the laboratory for multiple generations.

[0027] 2. Method

[0028] The test agents, imidacloprid, pirimicarb, dimethoate and beta-cypermethrin, were tested for toxicity using glass tube film method.

[0029] 2.1 Preparation and dilution of the original drug mother liquor

[0030] Dissolve the original medicine in acetone, put it in a medicine bottle after constant volume, seal it with a sealing film, and store it in a refrigerator at 4°C.

[0031] Before the toxicity test, the mother liquor of the original drug was diluted to various concentrations with acetone for use.

[0032] 2.2 Production of glass tube film

[0033] The glass tube used in the experiment (such as figure 1 (Shown) must go through strict washing to ensure that there is no drug residue. Pipette 220μL from ...

Embodiment 2

[0042] Example 2 Detection of resistance of the diagnostic dose to Rhopalosiphum padi

[0043] 1. Research object

[0044] The research object of this embodiment is the population to be tested of Rhopalosiphum padi in the field.

[0045] 2. Method

[0046] The test agents imidacloprid, antipyricarb, dimethoate and beta-cypermethrin were used to make glass tube film with the diagnostic dose of the invention, and the resistance level of Rhopalosiphum padi in the field was tested.

[0047] 2.1 Preparation and dilution of the original drug mother liquor

[0048] The original drug was dissolved in acetone, and the original drug mother liquor was diluted with acetone for the diagnostic dose obtained in Example 1 for use.

[0049] 2.2 Production of glass tube film

[0050] The glass tube used in the experiment (such as figure 1 (Shown) must go through strict washing to ensure that there is no drug residue. Pipette 220μL from the diluted drug solution into a glass tube with a diameter of 1.7cm an...

Embodiment 3

[0053] Example 3 Chemical control of diagnostic glass tubes to determine the resistance of Rhopalosiphum padi in the field

[0054] 1. Research object

[0055] The research object of this embodiment is the population to be tested of Rhopalosiphum padi in the field.

[0056] 2. Method

[0057] The test agents imidacloprid, antipyricarb, dimethoate and beta-cypermethrin were used to make glass tube film with the diagnostic dose of the invention, and the resistance of Rhopalosiphum padi in the field was measured.

[0058] 2.1 Operation steps

[0059] 1) According to the field sampling standards, take aphid-bearing wheat plants and bring them back indoors;

[0060] 2) Shake the wheat seedlings to make the aphids fall off the white paper, select the lively Rhopalosiphum padi into the diagnostic glass tube, 10-20 per tube (try to choose wingless adult aphids), seal with gauze, 3-5 for each agent Duplicate diagnostic reagent tubes;

[0061] 3) Put the diagnostic reagent tube containing aphids in...

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Abstract

The invention provides a method for detecting drug resistance of rhopalosiphum aphids. The method comprises the following steps: producing a diagnostic glass tube at a diagnostic dose of a drug; rapidly detecting the drug resistance level of a rhopalsiphum aphid population in a field, wherein the drug is imidacloprid, pirimicarb, omethoate or high-efficiency cypermethrin; the diagnostic dose of the drug is as follows: the diagnostic dose of the imidacloprid is 898 ng / cm<2>, the diagnostic dose of the pirimicarb is 18 ng / cm<2>, the diagnostic dose of the omethoate is 170 ng / cm<2>, and the diagnostic dose of the high-efficiency cypermethrin is 3077 ng / cm<2>. The method is easy and convenient to use and visual and clear in result, and is applicable to rapid field detection of the resistance level of the rhopalosiphum aphids; by the method, the defects that in the conventional biodetection method, a large number of samples are used, operations are cumbersome, much time is used and the like are overcome; the method can be used for rapidly detecting the resistance level of the rhopalosiphum aphid population in the field.

Description

Technical field [0001] The invention relates to a technical method for monitoring pesticide resistance of agricultural pests, in particular to a method for detecting pesticide resistance of Rhopalosiphum padi. Background technique [0002] Rhopalosiphum padi is one of the world's important agricultural pests that damage wheat. It is found in all wheat-producing countries in the world, and occurs in the north and south wheat regions of my country. Rhopalosiphum can not only directly pierce the nutrients of plants, causing severe water loss and malnutrition, but also spread viruses, causing greater indirect damage to plants and huge economic losses to agricultural production. For a long time, the control of Rhopalosiphum padi is mainly based on chemical control. Due to the large, continuous and unreasonable use of chemical insecticides, Rhopalosiphum padi in many countries and regions have produced common pesticides. Different degrees of drug resistance. [0003] The formation and ...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5085G01N2800/44
Inventor 高希武辛娟娟鲁艳辉刘晓岚张帅肖达杨婷王思一宋敦伦
Owner CHINA AGRI UNIV
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