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A method for rapid breeding of big cherry Gisela

A technology of Gisela and cherry, which is applied in the field of rapid breeding of large cherry Gisela, can solve the problems of slow reproduction speed, unstable technology, and inability to integrate science and technology, and achieve time saving, advanced technology, high promotion value and application value effect

Inactive Publication Date: 2016-08-31
SHAANXI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. Unstable technology
[0004] 2. There are few sources of cutting materials, and the materials cannot achieve the ideal breeding purpose
[0005] 3. Reproduction technology is a conventional method, which cannot be connected with modern advanced science and technology
Cutting propagation technology is applied, but the propagation speed is slow, which is far from meeting the production needs

Method used

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  • A method for rapid breeding of big cherry Gisela

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Embodiment Construction

[0034] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0035] A method for rapid breeding of large cherry Gisela, comprising the steps of:

[0036] 1), explant acquisition and detoxification

[0037] The explants should be detoxified and sterilized to obtain sterile shoot tips;

[0038] 2), explant induction culture

[0039] Explant induction medium: use 4 kinds of hormones 6-BA (6-benzylaminopurine), ZT (zeatin), KT (kinetin), IBA (indole butyric acid) combination, MS+6-BA0.5mg / L+ZT0.4mg / L+IBA0.1mg / L, induced and cultured "Gisela" explants for 30 days, and obtained new buds.

[0040] 3) Proliferation and cultivation of clustered buds

[0041] Choose to use MS as the basic medium, and use the combination of four hormones 6-BA (6-benzylaminopurine), ZT (zeatin), KT (kinin), and IBA (indolebutyric acid) to optimize the medium: Ms+6-BA0.5mg / L+ZT0.2mg / L+KT0.2mg / L+IBA0.1mg / L, inducing and culturing...

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Abstract

The invention discloses a method for rapidly breeding large cherry Gisela, which comprises the following steps: 1) obtaining an explant and eliminating virus; 2) inducing culture of the explants; 3) cultivating multiple shoots; 4) rooting; 4) hardening seedling; 6) cultivating in field. The invention combines laboratory clone technology and booth trainning method technology for breeding Gisela stock, thereby solving previous problems, such as slow breeding speed and bad stock variety, and compared to the cutting breeding, the breeding speed increased 30 times or more, and the invention has the advantages of advanced technology, saved time, higher popularization and application values with nursery stock production 0.1-1 million strains per year.

Description

technical field [0001] The invention relates to the field of cherry cultivation, in particular to a rapid breeding method for large cherry Gisela. Background technique [0002] The big cherry is native to Europe and is an excellent arbor fruit tree variety. Because the big cherry has many characteristics such as early fruit maturity, large fruit grains, gorgeous color, good taste, and high economic benefits, it is also rich in nutrient species, strong in health care, and deeply Popular with consumers. China belongs to the country of introduction and cultivation, and the speed of development is very fast. At the same time, big cherry is a fruit tree variety for grafting cultivation. In the production and cultivation process, the selection of rootstock varieties is very important. Because rootstock determines tree vigor, tree age, resistance, adaptability, and also affects high-yield performance, fruit quality, and flowering and fruiting time. For polyploid plants that cann...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 白文钊代贵华
Owner SHAANXI UNIV OF TECH
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