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Six-hole plate for cell culture

A cell culture and culture well technology, applied in the field of six-well plates, can solve the problems of mutual cross-contamination, waste of experimental consumables, affecting experimental results, etc., and achieve the effect of simplifying experimental steps, saving experimental consumables, and simplifying the operation process.

Inactive Publication Date: 2015-01-28
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The six-well plate that has been widely used in the laboratory has the following defects: 1. After washing the cells, the PBS solution (phosphate buffered saline) in each well needs to be sucked one by one, and the operation is time-consuming and laborious.
2. The cells in the six wells are divided into several groups according to different treatment schemes. When absorbing the PBS solution in the wells, each group needs a pipette. The experimental steps are cumbersome and waste experimental consumables.
3. Since each group needs a straw, there are many straws and the same shape, which is easy to be confused, resulting in operational errors and cross-contamination between groups, affecting the experimental results
4. Continuous washing with flowing PBS solution cannot be realized, and the washing efficiency is low

Method used

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  • Six-hole plate for cell culture
  • Six-hole plate for cell culture
  • Six-hole plate for cell culture

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Embodiment Construction

[0015] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0016] see figure 2 , image 3 , Figure 4 with Figure 5 , including a six-hole plate box body 1 and a cover 8, six culture holes 2 are arranged on the six-hole plate box body 1, a collection drainage groove 4 is arranged on the six-hole plate box body 1, and a collection drainage groove 4 port is provided There is a drainage port 5, and the six-hole plate box body 1 is provided with a drainage groove 3 connecting each culture hole 2 and the collective drainage groove 4, and a watershed 7 higher than the bottom surface of the culture hole 2 is provided in the drainage groove 3.

[0017] Preferably, the side of the watershed 7 facing the culture hole 2 is provided with a slope 6 connected to the culture hole 2 .

[0018] Preferably, the lid 8 is provided with a cover tongue 9 covering the drain opening 5 .

[0019] see figure 2 , imag...

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Abstract

The invention discloses a six-hole plate for cell culture, wherein a collecting drainage groove (4) is formed on the box body (1) of the six-hole plate; a drainage opening (5) is formed at the port of the collecting drainage groove (4); a drainage groove (3) for connecting each culture hole (2) with the collecting drainage groove (4) is formed on the box body (1) of the six-hole plate; a watershed (7) which is higher than the bottom surface of the culture hole (2) is arranged in the drainage groove (3). The six-hole plate for cell culture is low in cost, easy to manufacture and convenient to use; the brand new design is realized based on the structure of the existing six-hole plate; the washing liquid can be removed efficiency and quickly due to the addition of the drainage groove and the collecting drainage groove beside the holes; as a result, the liquid exchange method of absorbing and draining the waste liquid hole by hole at present is changed and experimental steps are simplified; the experimenter can save time and labor; in the meantime, experimental consumables can be saved and misoperation caused by mixing suction tubes can be avoided; continuous washing by using flowing PBS (Phosphate Buffer Solution) can be realized and the washing efficiency is high.

Description

technical field [0001] The invention relates to a six-hole plate for cell culture. Background technique [0002] Six-well plate is a common equipment in experiments such as cell culture, see figure 1 , the existing six-hole plate consists of a six-hole plate box body 1, a cover 2 and a culture hole 2 arranged on the six-hole plate box body 1. When doing cell culture, it is necessary to regularly wash and change the medium for the cells in the six-well plate. The six-well plate widely used in the laboratory has the following defects: 1. After the cells are washed, the PBS solution (phosphate buffered saline) in each well needs to be sucked one by one, and the operation is time-consuming and laborious. 2. The cells in the six wells are divided into several groups according to different treatment schemes. When absorbing the PBS solution in the wells, each group needs a pipette. The experimental steps are cumbersome and waste experimental consumables. 3. Since each group need...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/12
Inventor 王晓艳卜迟斌钱明永袁维黄铭玉艾飞艳任为国代贺龙
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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