36results about How to "Reduce lab consumables" patented technology

The invention discloses a screening method for 110 types of medicines in feed, which specifically includes the following steps: preparation of standard work solution, pre-analysis treatment of sample to be assayed, creation of database and qualitative analysis. By utilizing the high-sensitivity MSMS (Tandem Mass Spectrometry) function of a liquid chromatography-tandem quadrupole time-of-flight mass spectrometer, the screening method can accurately determine parent ions and daughter ions in medicines and rapidly determine added medicine ingredients, moreover, an assay method for a variety of antibacterial medicines in feed is also established, and for a variety of added antibacterial medicines in the feed industry in China, the screening method has a highly efficient, accurate and precise assay measure, and is at the advanced level in china at present. The screening method established by the invention greatly solves the phenomenon of arbitrary addition in the feed industry, the more precise and accurate assay method is perfected for the inspection department, more time is saved, most of experimental consumables are saved, and the invention makes an immeasurable contribution to food safety and human healthy life.

The invention discloses an erosion ditch monitoring method based on close-range photogrammetry. The erosion ditch monitoring method comprises the steps that a photographing target is arranged around an erosion ditch, and a total station or an RTK-GPS is adopted for measuring a target center point, and position information is obtained; a non-measuring common digital camera is adopted to rotate around the erosion ditch clockwise (anticlockwise) and manual photographing is carried out; the photo is guided into Agisoft Photoscan software, and the sparse point cloud, dense point cloud and grids are established through camera calibration, photo splicing, target detection operation and the like, and the coordinates of the target point are guided to obtain a digital elevation model and a digital positive projection image of the erosion ditch; and the digital elevation model of the high-precision erosion ditch is guided into ArcGIS 10.2 software, and form parameters and erosion amount information of the erosion ditch are extracted. The method has the advantages that the dependence and the requirement degree of the instrument are low, the cost is low, the experimental consumable is small, the high-quality digital elevation model data can be quickly obtained, and the information of the erosion ditch can be conveniently and accurately extracted.

The invention discloses a pair of RT-PCR ( reverse transcription-polymerase chain reaction) primers and a kit for detecting high-pathopoiesia pig blue ear disease and classic pig blue ear disease. The upstream primer nucleotide sequence of the primers is GGCGACAATGTCCCTAAC, the downstream primer nucleotide sequence of the primers is GATGGCTTGAGCTGAGTAT, and the kit contains the primers. The primers and the kit for detecting high-pathopoiesia porcine reproductive andrespiratory syndrome and classic porcine reproductive andrespiratory syndrome have the advantages of high sensibility, strong specificity, rapidness and convenience, low detecting cost and the like.

A preparing method of a human DCCIK immunocompetent cell is disclosed. The method includes steps of: (1) separating a mononuclear cell from human peripheral blood; (2) inoculating the mononuclear cell to a culture medium suitable for lymphocyte culturing, adding IFN[gamma], IL-2, an anti-CD3 monoclonal antibody, GM-CSF and IL-4, and culturing for 3-5 days; (3) adding TNF[alpha], and continuously culturing for 1-2 days; and (4) adding IL-2, and continuously culturing for 7-10 days to obtain the DCCIK cell. A composition of various cytokines and stimulating factors is added directly into the mononuclear cell so that the DC cell and lymphocyte induce at the same time and mutually stimulate maturity, thus simplifying operation steps, reducing experiment consumable items and shortening cell culturing time. In addition, normalized experiment process technology can be easily mastered, thus facilitating clinic popularization and application.

The invention discloses an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting European and American porcine reproductive and respiratory syndrome viruses and a kit. The nucleotide sequence of an upstream primer of the primer is 5'-ATGTGGGATAGGGTGGACA-3', and the nucleotide sequence of a downstream primer is 5'-AAGAACCAGAAAAGACACCCAA-3'; the kit comprises the primer. The European and American RT-PCR primer and the kit are adopted for detecting whether the European and American porcine reproductive and respiratory syndrome viruses are positive, and have the advantages of high sensitivity, strong specificity, fastness, convenience and low detection cost and the like.

The invention provides an in-loop simulation method and system oriented to an intelligent logistics vehicle. According to the in-loop simulation method and system oriented to the intelligent logisticsvehicle, an experiment environment setting module, an image acquisition module, an image sensing module, a decision control module, a driving control module, an information checking module, a wireless automatic reception module and a human-vehicle interaction module are provided. According to the in-loop simulation method and system oriented to the intelligent logistics vehicle, contents including self-service operation, information confirmation and storage delivery on the intelligent logistics vehicle by users can be realized in an experiment platform, and research personnel is effectively helped to develop or test interaction content and interaction effect between the intelligent logistics vehicle and the users in a laboratory after computer simulation and before falling of articles onthe ground by the intelligent logistics vehicle.

The invention relates to an experimental method for preventing generation and growth of liver cancer by using umbilical cord mesenchymal stem cells. For the first time, an application of the umbilicalcord mesenchymal stem cells to prevent the generation and growth of liver cancer is provided. The UC-MSCs cell preparation method is provided, and the experimental data of the dose of 1*104 cells / treatment course and administration method of the UC-MSCs cells for preventing liver cancer are provided. A UC-MSCs preparation is transplanted firstly, then the liver cancer cells are implanted, and thetumor growth of NOD / SCID mice is observed and compared, the method proves that the umbilical cord mesenchymal stem cells can prevent the generation and development of liver cancer and the dosage of UC-MSCs is provided, the self-renewal and damage repair functions of UC-MSCs in the body's adult organ tissue can be maximized, the body's immunity is improved, and the generation of liver cancer can be prevented, which is beneficial to play a more significant and stable role in later research and clinical applications.

The invention discloses a general RT-PCR (reverse transcription-polymerase chain reaction) primer and a general kit for detecting foot and mouth disease viruses. The nucleotide sequence of an upstream primer of the primer is 5'-CTGGGCCCTTCTTCTTC-3', and the nucleotide sequence of a downstream primer of the primer is 5'-GCTGTTTCTCTGCTCTCTC-3'. The kit comprises the primer. The general RT-PCR primer and the kit which are disclosed by the invention and are used for detecting O-type, A-type and sub-I viruses of foot and mouth diseases have the advantages of high sensitivity, high specificity, quickness, simplicity and the like.

The invention discloses an open multifunctional test platform related to gas reaction. A gas source library comprises a plurality of gas sources. A gas distribution system comprises a plurality of mixing tanks and gas purging tanks which are connected with the gas sources through a plurality of mass flow meters, and the mixing tanks are connected with inlet ends of a reaction system through a plurality of flow distributors. The reaction system comprises a plurality of reactor assemblies connected with the flow distributors, wherein the reactor assemblies are installed on support or supportingbases, the outlet ends of the reactor assemblies are sequentially connected with a separating tank and a multi-channel switching valve through a pressure buffer tank, and the multi-channel switching valve makes the reactor assemblies connected in parallel or in series. A detecting and monitoring system comprises temperature probes, pressure meters and gas concentration detectors distributed in parts, matched display instruments and a heating controller. The shortcoming of low researching and testing efficiency of a traditional single set of equipment is overcome, and the problems of few gas paths, large batch-type testing work amount, long period and the like of an existing experimental device related to gas reaction are solved.

The invention relates to an economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device. The invention relates to processes of intubation needle manufacturing, mouse fixing device manufacturing, mouse tracheal intubation operation and the like in a silicosis fibrosis model. Specifically, each mouse is fixed on a self-made mouse fixing plate after being anesthetized, the glottis is exposed, and an SiO2 suspension is quantitatively dripped into the trachea of the mouse by using a self-made trachea cannula needle. Intubation is conducted once every 5-7 days for one month, and the silicosis fibrosis occupational disease model is successfully established. The lung tissue of the mouse is taken for lung morphology and pathology analysis, and the result proves that the method is simple to operate and low in cost, the success rate of the silicosis fibrosis occupational disease mouse model is high, and the fibrosis degree is progressive. The invention provides a good experimental animal model for further research on the pathogenesis of the silicious dust-induced pulmonary fibrosis and drug screening.

The invention discloses an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting poultry-like type H1N1 swine influenza virus and a kit. The RT-PCR primer comprises two pairs: primers for H1 gene and primers for N1 genes, wherein the nucleotide sequence of an upstream primer Hu on the primers for the H1 gene is 5'-GACTCTAGATTTCCATGACCTT-3', and the nucleotide sequence of a downstream Hd is 5'-ACCCATTAGAACACATCCAGAA-3'; the nucleotide sequence of an upstream primer Nu on the primers for the N1 gene is 5'-TCCAAGGGGGATGTGTTTG-3', and the nucleotide sequence of a downstream primer Nd is 5'-GACCAAGCGACTGACTCAA-3'; the kit comprises the primer. The RT-PCR primer and the kit are adopted for detecting the poultry-like type H1N1 swine influenza virus and have the advantages of high sensitivity, strong specificity, fastness and convenience and the like.

The invention discloses a method for rapidly determining content of table salt in sauce-marinated pork and / or marinated soup and an application. The method comprises the steps as follows: a work curveis established; a sample is treated; the conductivity of the sample is determined; the conductivity of a sample solution is determined; the content of the table salt in the sample is calculated. Thesample can be determined after water dissolution (extraction) treatment, stirring and filtration are performed, the method is simpler than a sample digestion treatment process in national standard detection, and the effect of a blank sample can be ignored during determination with the method. The method does not have too many limitation conditions, and for other samples, such as a series of propersamples including chicken, chicken soup, sweet soup, flavoring soup and the like which are finally treated to form to-be-determined samples like marinated soup or sauce-marinated pork with certain means, and the determination method may be superior to other determination methods.

The invention discloses a method for improving the repeatability of human albumin aluminum residue. The method for testing human blood albumin aluminum residue comprises the following steps: S1, solution preparation, wherein a diluent is prepared from 0.15% to 0.25% of a non-ionic surfactant solution, and a standard aluminum solution is prepared by the steps of preciously measuring 0.1ml of 100 micron / ml standard aluminum solution, placing in a 100ml volumetric flask and diluting with deionized water; S2, detection solution preparation, wherein 2.5ml of a diluent is taken as a blank solution,2.3ml of the diluent is added into 0.2ml of a test solution to obtain a test solution, and a standard aluminum plus test solution is prepared by the steps of adding 1.8ml of the diluent into 0.2ml ofthe test solution and 0.5ml of the standard aluminum solution; and S3, testing, namely with an atomic absorption spectrophotometer for testing, setting graphite furnace parameters, accurately measuring the blank solution, the test solution and the standard aluminum plus test solution, and injecting the solutions into the instrument. The method significantly increases the repeatability of human albumin aluminum residue.

The invention relates to a top cover type cleaning device. The top cover type cleaning device comprises a barrel body, a top cover and a hinged part, wherein a washing cavity is formed in the barrel body; the top cover is hinged to the upper portion of the washing cavity and is used for sealing the washing cavity; a spraying structure is formed on the side, facing the washing cavity, of the top cover, so that the washing device can carry out spraying from the top cover to the washing cavity, and the spraying area and the washing effect formed by the top spraying mode are far superior to thoseof a side spraying mode; one end of the hinged part is fixed with the barrel body, and the other end of the hinged part is fixed with the top cover; and the hinged part comprises a memory alloy hinge,and opening or closing of the top cover can be controlled by controlling opening and closing movement of the memory alloy hinge, so that the loss rate of parts is greatly reduced, automatic opening and closing of the top cover are guaranteed, and experimental consumables are reduced.

The invention discloses a modified polyurethane foam and a preparation method and applications thereof. Expanded perlite, rick husk ash particles, and hydroxyl terminated poly(epoxy chloropropane) areadopted to carry out modification to obtain modified polyurethane foam; the acoustic property and mechanical properties are all improved; and the modified polyurethane foam is a light and vibration-proof material having a good performance of absorbing medium-low frequency sound and can be used to prepare a sound absorption board of a power capacitor. The modified polyurethane foam is manufacturedaccording to an optimal ratio; the average low / medium frequency sound absorbing performance is obviously enhanced and can reach 0.54; and the average noise insulation factor reaches 12.13 dB. The foam mainly absorbs the capacitor noise with frequencies of 500Hz, 600Hz, and 700Hz, and the sound absorbing coefficient is obviously increased. The wastes are converted into valuable resources, and theeconomic benefits and environmental benefits are increased.

The invention relates to an automatic device for wave-shifted optical fiber bending. The device comprises a flat plate, wherein two ends of the flat plate are connected with bending plates which can only rotate by 90 degrees in a hinged mode correspondingly, the flat plate is provided with a first optical fiber groove group matching a wave-shifted optical fiber side by side, one sides of the bending plates are provided with bending handles correspondingly, the other sides of the bending plates are provided with pressing devices matching the wave-shifted optical fiber correspondingly, a bendingdevice is arranged on the side, not provided with the optical fiber groove group, of the flat plate and comprises a bending driver arranged on the flat plate, bending output shafts are arranged on the front side and the rear side of the bending driver correspondingly, the two bending output shafts are symmetrical with respect to the center of a center point of the flat plate, the bending output shafts are sleeved with bending rotating cylinders, bending pull ropes are fixed to the bending rotating cylinders, and the other ends of the bending pull ropes are arranged on pull rope installation bases on the side faces of the bending plates. According to the device, the optical fiber water bath bending consistency is better, the bending efficiency is higher, the bending reject ratio is reduced, and later experimental data is more accurate and controllable.

The invention discloses a microsatellite triple ternary PCR detection method of marsupenaeus japonicas. The method includes the steps that three pairs of PCR primers are designed and synthesized; the total DNA is extracted; combination, a system and a reaction program of the ternary PCR reactions are provided; PCR products are detected. The efficiency of the microsatellite triple ternary PCR detection method of marsupenaeus japonicas can be tripled compared with an original method. The method has the advantages that the cost and time are saved, and the method is efficient, economical, high in repeatability, simple, feasible, beneficial to detection and the like and can be applied and popularized with respect to breeding management, family identification, individual recognition, genetic relationship identification, improved breed breeding, linkage map construction, genetic diversity detection and the like of marsupenaeus japonicas.

The invention relates to the field of teaching equipment, and aims to provide a gravitational acceleration measuring method and a device thereof. According to the gravitational acceleration measuring method and the device, a laser transmitting device replaces a traditional ticker timer, thereby realizing no-contact ticking, reducing effect of a friction resistance of a traditional tape belt, and realizing more accurate experiment result. The gravitational acceleration measuring method has beneficial effects of simple structure and convenient operation. Ticking on a falling object which is coated with fluorescent powder is realized through the laser transmitting device, thereby realizing visual and obvious phenomenon and high stability. The gravitational acceleration measuring method is suitable for self research and self experience of a student, thereby improving persuasiveness in teaching. The falling object can automatically return to normal after a certain time period. High repeatability is realized, and furthermore consumable materials are greatly saved. Through a strange optical phenomenon of making the falling object emit light by means of the laser transmitting device, attention of the student can be focused to the experiment, thereby improving studying enthusiasm of the student and improving teaching effect.

The invention belongs to the field of PCR (Polymerase Chain Reaction) detection, and particularly relates to a triple PCR detection method of muscovy duck parvovirus, goose parvovirus and avian adenovirus type 4. The triple PCR detection method comprises the following steps: S1, respectively designing three pairs of specific amplification primers according to gene conserved sequences of the muscovy duck parvovirus, the goose parvovirus and the avian adenovirus type 4; S2, extracting DNAs of the muscovy duck parvovirus, the goose parvovirus and the avian adenovirus type 4; S3, mixing all the DNAs obtained in S2 and then carrying out PCR amplification; S4, detecting PCR products in S3 through electrophoresis. The triple PCR detection method established can simultaneously detect the muscovy duck parvovirus, the goose parvovirus and the avian adenovirus type 4; the minimum detection amounts of three viral nucleic acid templates are 2pg / mu L, 20pg / mu L and 2pg / mu L respectively.

The invention discloses equipment for observing a directional solidification process in real time. The equipment comprises a CCD micro-camera, a constant temperature water tank, a heating zone, a coldzone, a linear displacement table, a rubber pipe, a glass container, a heater, a heat insulation plate, a shock absorption plate, a computer, a cold light source, a glass plate, a connecting rod, a supporting rod, a temperature sensor I, a groove, a water inlet, a water outlet, a peristaltic pump, a temperature sensor II and a vacuum pump. The equipment for observing the directional solidification process in real time can directly observe the eutectic solidification of an alloy and the forming process of a porous material, in-depth study of a eutectic growth mechanism and a formation mechanism of the porous material can be researched, and thus the development of the material and condensed matter physics can be further promoted, and an eutectic and porous composite material and other materials with better performance are prepared.

The invention discloses an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting porcine Kobu virus and a kit. The nucleotide sequence of an upstream primer of the primer is 5'-AACTCCAGCTTTCCCTCCT-3', and the nucleotide sequence of a downstream primer is 5'-TGGCCACCTGCAAAGTCA-3'; the kit comprises the primer. The RT-PCR primer and the kit are adopted for detecting the porcine Kobu virus of the pig, and have the advantages of high sensitivity, strong specificity, fastness and convenience and the like.

The invention provides a device and a method for toxicological research of plant roots. The device comprises a toxicological test chamber and a culture chamber which are detachably connected, wherein the culture chamber is arranged above the toxicological test chamber for toxicological test, the toxicological test chamber comprises a body, at least two butted first partition plates are arranged in the body, the body is divided into at least two cavities by the first partition plates, a sample cavity is reserved at the butted part of the butted first partition plates, and the sample cavity is used for accommodating a plant root. The device has characteristics of simplicity, rapidness, convenience and safety, is not only suitable for research of single factor influence, but also can be used for research of multi-element combination effect, reduces experimental consumables, reduces experimental risks, and improves experimental efficiency.

The invention discloses a plasma based object characterization detection method and device. Physical characteristics of the surface of a to-be-detected object are represented according to interactionforce between plasma and the to-be-detected object. The plasma is taken as a probe, when approaching the surface of the object, the plasma has interaction with the surface of the object, the contour of the surface of the object can be obtained by measuring influence of the interaction between the plasma and the surface of the object, and characterization detection for the object is realized.

The invention discloses a method for determining the content of volatile fatty acid in ruminant rumen fluid. The method comprises the following steps: preparing a standard solution, setting headspace sampling conditions, setting gas chromatography conditions, treating a sample and the like. According to the present invention, the VFA content in the ruminant rumen fluid is determined by using the headspace gas chromatography, the sample pretreatment is not required, and the sample introduction analysis is directly performed by using the headspace bottle so as to furthest retain the original components, and the detection result is closer to the real components of the sample. Compared with common sample introduction analysis, pollution of sample impurities to the chromatographic column is effectively reduced, the maintenance cost is reduced, and meanwhile, experimental consumables used for sample pretreatment are saved.

The invention provides a domestication method of HEK293T cells and application of the domestication method. The domestication method comprises a suspension and serum-free culture domestication method of the HEK293T cells, a cell line obtained by the domestication method and application of the cell line. The domestication method is few in passage times and high in domestication efficiency, and the obtained cells are high in viability, good in dispersity and stable in growth state and can meet the requirements of commercialized and large-scale production.