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Method for improving repeatability of human albumin aluminum residue

A technology of human serum albumin and residual quantity, which is applied in the direction of measuring devices, preparation of test samples, color/spectral characteristic measurement, etc., can solve problems such as increased frequency of damage to graphite tubes, increased inspection costs of enterprises, inability to provide guidance for production, etc. , to achieve the effect of reducing carbon residue accumulation, reducing instrument maintenance finished products, and saving experimental consumables

Inactive Publication Date: 2018-11-13
HUALAN BIOLOGICAL ENG CHONGQING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, protein substances such as human blood albumin are non-metallic and have a certain impact on the instrument, especially the impact on the graphite tube. If the repeatability is not good, the test results are inconsistent and cannot provide guidance for production.
On the other hand, the damage frequency of graphite tubes increases, which increases the inspection cost of enterprises

Method used

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  • Method for improving repeatability of human albumin aluminum residue
  • Method for improving repeatability of human albumin aluminum residue
  • Method for improving repeatability of human albumin aluminum residue

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] Preparation of blank solution: take 2.5ml diluent as blank solution;

[0027] Need testing solution: take need testing solution 0.2ml and add 2.3ml diluent, as need testing solution;

[0028] Standard aluminum plus test solution: take 0.2ml of test solution, then take 0.5ml of standard aluminum solution, add 1.8ml diluent, as standard aluminum plus test solution;

[0029] S3 detection: detect with an atomic absorption spectrophotometer, set the parameters of the graphite furnace, accurately measure the blank solution, the test solution, the standard aluminum plus the test solution and inject it into the instrument.

[0030] The nonionic surfactant in step S1 is alkyl polyoxyethylene ether.

[0031] The volume of surfactant added in step S1 is 2.0 ml.

[0032] The alkyl polyoxyethylene ether nonionic surfactant is polyethylene glycol octylphenyl ether.

Embodiment 1

[0034] S1 solution preparation:

[0035] Diluent: Add 1.5ml of polyethylene glycol octylphenyl ether to 1000ml of deionized water to make a 0.15% polyethylene glycol octylphenyl ether solution (V / V);

[0036] Standard aluminum solution: Accurately measure 0.1ml of 100μg / ml standard aluminum solution, put it in a 100ml volumetric flask, dilute and dissolve with deionized water;

[0037] S2 detection solution preparation:

[0038] Preparation of blank solution: take 2.5ml diluent as blank solution;

[0039] Need testing solution: take need testing solution 0.2ml and add 2.3ml diluent, as need testing solution;

[0040] Standard aluminum plus test solution: take 0.2ml of test solution, then take 0.5ml of standard aluminum solution, add 1.8ml diluent, as standard aluminum plus test solution;

[0041] S3 detection: detect with an atomic absorption spectrophotometer, set the parameters of the graphite furnace, accurately measure the blank solution, the test solution, the standard...

Embodiment 2

[0048] S1 preparation solution preparation:

[0049] Diluent: Add 2.0ml of polyethylene glycol octylphenyl ether to 1000ml of deionized water to make a 0.20% polyethylene glycol octylphenyl ether solution (V / V);

[0050] Standard aluminum solution: Accurately measure 0.1ml of 100μg / ml standard aluminum solution, put it in a 100ml volumetric flask, dilute and dissolve with deionized water;

[0051] S2 detection solution preparation:

[0052] Preparation of blank solution: take 2.5ml diluent as blank solution;

[0053] Need testing solution: take need testing solution 0.2ml and add 2.3ml diluent, as need testing solution;

[0054] Standard aluminum plus test solution: take 0.2ml of test solution, then take 0.5ml of standard aluminum solution, add 1.8ml diluent, as standard aluminum plus test solution;

[0055] S3 detection: detect with an atomic absorption spectrophotometer, set the parameters of the graphite furnace, accurately measure the blank solution, the test solution, ...

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Abstract

The invention discloses a method for improving the repeatability of human albumin aluminum residue. The method for testing human blood albumin aluminum residue comprises the following steps: S1, solution preparation, wherein a diluent is prepared from 0.15% to 0.25% of a non-ionic surfactant solution, and a standard aluminum solution is prepared by the steps of preciously measuring 0.1ml of 100 micron / ml standard aluminum solution, placing in a 100ml volumetric flask and diluting with deionized water; S2, detection solution preparation, wherein 2.5ml of a diluent is taken as a blank solution,2.3ml of the diluent is added into 0.2ml of a test solution to obtain a test solution, and a standard aluminum plus test solution is prepared by the steps of adding 1.8ml of the diluent into 0.2ml ofthe test solution and 0.5ml of the standard aluminum solution; and S3, testing, namely with an atomic absorption spectrophotometer for testing, setting graphite furnace parameters, accurately measuring the blank solution, the test solution and the standard aluminum plus test solution, and injecting the solutions into the instrument. The method significantly increases the repeatability of human albumin aluminum residue.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a method for improving the repeatability of aluminum residues in human serum albumin. Background technique [0002] Aluminum ions in the human body may have the potential danger of causing osteomalacia, hypohemochromic anemia, progressive intellectual loss in the elderly and chronic renal impairment. In some industries with relatively high aluminum content, the blood aluminum content will be tested regularly to reduce the harm of aluminum to the human body. The aluminum content in each person's body will vary due to different living habits, occupations, and living environments. Human albumin is mainly used for blood loss trauma, shock caused by burns, increased intracranial pressure caused by cerebral edema and injury, edema or ascites caused by liver cirrhosis and nephropathy, prevention and treatment of hypoproteinemia, neonatal hyperbilirubinemia It is used for cardiopulmonary b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N21/31
CPCG01N1/38G01N21/3103
Inventor 张穹刘利张宝献郭心怡杨柳张建璀
Owner HUALAN BIOLOGICAL ENG CHONGQING
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