RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting porcine Kobu virus and kit
A RT-PCR, kit technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of research, high morbidity and mortality, economic losses, etc., to improve work efficiency, The effect of avoiding false negative results and reducing the cost of testing
Inactive Publication Date: 2014-02-12
广西壮族自治区动物疫病预防控制中心 +1
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Problems solved by technology
From the winter of 2010 to the spring of 2011, severe infectious diarrheal diseases occurred successively in pig farms all over the country. The application of various antibiotics was ineffective, and the morbidity and mortality were high, causing significant economic losses.
Since porcine KoBu virus is a newly discovered virus, related research is relatively scarce
Method used
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[0040] The present invention will be further described below in conjunction with embodiment, but do not limit protection scope and application scope of the present invention:
[0041] 1. Kit for detecting porcine KoBu virus
[0042] A kit for detecting porcine KoBu virus, including the following reagents, see Table 2 for details. The primers were designed by our laboratory and synthesized by Bao Bioengineering (Dalian) Co., Ltd. Other reagents can be purchased from the market.
[0043] Table 2 detects the kit of porcine KoBu virus
[0044]
[0045]
[0046] 2. How to use the kit
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The invention discloses an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer for detecting porcine Kobu virus and a kit. The nucleotide sequence of an upstream primer of the primer is 5'-AACTCCAGCTTTCCCTCCT-3', and the nucleotide sequence of a downstream primer is 5'-TGGCCACCTGCAAAGTCA-3'; the kit comprises the primer. The RT-PCR primer and the kit are adopted for detecting the porcine Kobu virus of the pig, and have the advantages of high sensitivity, strong specificity, fastness and convenience and the like.
Description
technical field [0001] The invention belongs to the technical field of animal virus molecular biological detection, in particular to a pair of RT-PCR primers and a kit for detecting porcine Kobu virus. Background technique [0002] KoBu virus (KoBuvirus) belongs to the Picornaviridae family, and is a virus of the same family as foot-and-mouth disease. It is a non-encapsulated single-stranded RNA virus with a genome size of 8.2kb to 8.3kb, including a large open reading frame encoding a polymeric protein ( ORF). Polymeric protein is composed of 3 structural proteins (VP0, VP3, VP1) and 7 non-structural proteins (2A-2C, 3A-3D). It has been confirmed that KoBu virus is divided into at least 3 species, including human KoBu virus (also known as Aichi virus), bovine KoBu virus and porcine KoBu virus. Human KoBu virus was isolated from the feces of diarrhea patients by Japanese scholars in 1991. The word KoBu comes from Japanese, meaning "spherical" or "protrusion". Human infecti...
Claims
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Login to View More IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/686C12Q1/70C12Q2531/113
Inventor 熊毅伍和明韦达有梁丹洁冯淑萍颜健华
Owner 广西壮族自治区动物疫病预防控制中心
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