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Economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device

A pulmonary fibrosis, mouse model technology, applied in the medical and experimental fields, can solve the problems of difficult to quantify pathogenic substances, difficult to ensure reliability, uneven pathological degree, etc., and achieves short experimental period, high success rate, and operation easy effect

Pending Publication Date: 2022-01-04
ANHUI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can accurately observe the airway, it does more damage to the mice, and the mice can hardly tolerate multiple operations, and are prone to infection and death, which not only causes economic losses, but also affects the progress of subsequent experiments.
In addition, there is a non-invasive spray inhalation of pathogenic substances for the construction of pulmonary fibrosis models. This method is difficult to quantify the pathogenic substances, and it is easy to lead to uneven pathology of the model, and it is difficult to ensure the credibility of subsequent experiments.

Method used

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  • Economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device
  • Economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device
  • Economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Step 1 Preparation before experiment

[0026] 1. Purchase, Breeding and Free Grouping of Mice

[0027] Thirty SFP-grade male mice, weighing 23±2g, were purchased from the Animal Experiment Center of Anhui Medical University. Afterwards, they were directly raised in the SPF level laboratory center of the Experimental Animal Center of Anhui University of Science and Technology. All experimental mice lived in a specific aseptic environment, the temperature was controlled at 25±2°C, the humidity was within the range of 60±10%, the light and dark cycle was 12h / 12h, and the conditions allowed the mice to eat and drink freely. After one week of feeding, they were randomly divided into model group and normal group. In addition, all procedures of this experiment were approved by the Biological Research Ethics Committee of Anhui University of Technology.

[0028] 2. Homemade mouse fixation plate

[0029] Remove the connecting plastic rods at the four corners of the purchased ...

Embodiment 2

[0051] Step 1 Preparation before experiment

[0052] 1. Purchase, Breeding and Free Grouping of Mice

[0053] Thirty SFP-grade male mice, weighing 23±2g, were purchased from the Animal Experiment Center of Anhui Medical University. Afterwards, they were directly raised in the SPF level laboratory center of the Experimental Animal Center of Anhui University of Science and Technology. All experimental mice lived in a specific aseptic environment, the temperature was controlled at 25±2°C, the humidity was within the range of 60±10%, the light and dark cycle was 12h / 12h, and the conditions allowed the mice to eat and drink freely. After one week of feeding, they were randomly divided into model group and normal group. In addition, all procedures of this experiment were approved by the Biological Research Ethics Committee of Anhui University of Technology.

[0054] 2. Homemade mouse fixation plate

[0055] Remove the connecting plastic rods at the four corners of the purchased ...

Embodiment 3

[0071] Step 1 Preparation before experiment

[0072] 1. Purchase, Breeding and Free Grouping of Mice

[0073] Thirty SFP-grade male mice, weighing 23±2g, were purchased from the Animal Experiment Center of Anhui Medical University. Afterwards, they were directly raised in the SPF level laboratory center of the Experimental Animal Center of Anhui University of Science and Technology. All experimental mice lived in a specific aseptic environment, the temperature was controlled at 25±2°C, the humidity was within the range of 60±10%, the light and dark cycle was 12h / 12h, and the conditions allowed the mice to eat and drink freely. After one week of feeding, they were randomly divided into model group and normal group. In addition, all procedures of this experiment were approved by the Biological Research Ethics Committee of Anhui University of Technology.

[0074] 2. Homemade mouse fixation plate

[0075] Remove the connecting plastic rods at the four corners of the purchased ...

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Abstract

The invention relates to an economical quantitative non-invasive silicious dust-induced pulmonary fibrosis occupational disease mouse model establishing method and preparation device. The invention relates to processes of intubation needle manufacturing, mouse fixing device manufacturing, mouse tracheal intubation operation and the like in a silicosis fibrosis model. Specifically, each mouse is fixed on a self-made mouse fixing plate after being anesthetized, the glottis is exposed, and an SiO2 suspension is quantitatively dripped into the trachea of the mouse by using a self-made trachea cannula needle. Intubation is conducted once every 5-7 days for one month, and the silicosis fibrosis occupational disease model is successfully established. The lung tissue of the mouse is taken for lung morphology and pathology analysis, and the result proves that the method is simple to operate and low in cost, the success rate of the silicosis fibrosis occupational disease mouse model is high, and the fibrosis degree is progressive. The invention provides a good experimental animal model for further research on the pathogenesis of the silicious dust-induced pulmonary fibrosis and drug screening.

Description

technical field [0001] The invention belongs to the field of medicine and experiment technology, and specifically relates to an economical, quantitative and non-invasive method for establishing a mouse model of occupational disease of pulmonary fibrosis caused by silica dust and a preparation device. Background technique [0002] Pulmonary fibrosis is a chronic progressive lung disease that is difficult to cure and has a high mortality rate. It is a respiratory disease that causes progressive pulmonary dysfunction due to excessive collagen deposition and alveolar structural disorder due to abnormal tissue repair. The main pathogenic factors are smoking, drugs, dust, bacterial or viral infection (such as COVID-19). Pneumoconiosis is a widespread and serious lung disease caused by dust. About 90% of newly diagnosed occupational diseases in my country are pneumoconiosis, and the number of pneumoconiosis patients and dust exposure ranks first in the world, among which the incid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 姚京京段聪慧崔世华胡东齐浩然田保刘新月王紫萍王亚洲
Owner ANHUI UNIV OF SCI & TECH
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