Method for determining content of volatile fatty acid in ruminant rumen fluid
A technology of volatile fatty acids and ruminants, which is applied in the field of animal husbandry to achieve the effects of saving experimental consumables, reducing maintenance costs, and reducing pollution
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Embodiment 1
[0032] A method for determining the content of volatile fatty acids in rumen juice of ruminants, comprising the steps of:
[0033] S1, standard solution preparation
[0034] Accurately weigh the standard products of acetic acid, propionic acid, and butyric acid, and make up to volume with pure water to prepare a mixed standard stock solution of 320mmol / L acetic acid, 32mmol / L propionic acid, and 32mmol / L butyric acid for use; pipette different volumes respectively The standard stock solution is configured as a mixed standard solution with a concentration of 80%, 100% and 120% of the measured concentration of the sample;
[0035] S2, headspace sampling conditions
[0036] Headspace sampler conditions: Equilibrium temperature 85°C, sample flow path temperature 180°C, transfer line temperature 200°C, equilibration time 30min, injection time 1min;
[0037] S3, gas chromatography conditions
[0038] The chromatographic column is SH-Stabilwax quartz capillary column; the temperat...
Embodiment 2
[0051] The present embodiment provides a kind of concrete test method as follows:
[0052] A method for determining the content of volatile fatty acids in rumen juice of ruminants, comprising the steps of:
[0053] 1 Materials and methods
[0054] 1.1 Instruments and reagents
[0055]HSS 86.50 headspace sampler (DANI, Italy); GC-2010Plus gas chromatograph (Shimadzu, Japan), equipped with hydrogen flame ionization detector (FID); Eppendorf series pipettes; From Feihong Packaging Materials Factory (Nantong City, Jiangsu Province); Glacial acetic acid, propionic acid, and butyric acid are all chromatographically pure, purchased from Aladdin Company (Shanghai); ethanol, propanol, and butanol are all chromatographically pure, purchased from Bailingwei Company (Beijing); air, nitrogen, and hydrogen were purchased from Hangzhou Jingong Materials Co., Ltd.; ultrapure water was produced by NANO pure UV / UF ultrapure water system.
[0056] 1.2 Standard solution preparation
[0057] A...
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