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General RT-PCR (reverse transcription-polymerase chain reaction) primer and kit for detecting foot and mouth disease viruses

A technology of RT-PCR and foot-and-mouth disease virus, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of easy false positives, difficult to guarantee specificity, false negatives, etc., and avoid false negative results , reduce testing costs and improve work efficiency

Inactive Publication Date: 2014-03-12
广西壮族自治区动物疫病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The purpose of these methods is to detect the corresponding antibodies, which belong to serological detection methods. In view of the high variability and crossover of FMD virus, its specificity is difficult to guarantee, and false positives and false negatives are prone to occur. Therefore, the establishment of effective diagnostic methods is very important for the prevention and treatment of FMD. of great significance

Method used

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  • General RT-PCR (reverse transcription-polymerase chain reaction) primer and kit for detecting foot and mouth disease viruses
  • General RT-PCR (reverse transcription-polymerase chain reaction) primer and kit for detecting foot and mouth disease viruses
  • General RT-PCR (reverse transcription-polymerase chain reaction) primer and kit for detecting foot and mouth disease viruses

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Embodiment Construction

[0042] The present invention will be further described below in conjunction with embodiment, but do not limit protection scope and application scope of the present invention:

[0043] 1. General-purpose kit for detecting foot-and-mouth disease virus

[0044] A general-purpose test kit for detecting foot-and-mouth disease virus, including the following reagents, see Table 2 for details, primers are designed by the laboratory, and other reagents can be purchased from the market.

[0045] Table 2 detects the kit of general type of foot-and-mouth disease virus

[0046]

[0047]

[0048] 2. How to use the kit

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Abstract

The invention discloses a general RT-PCR (reverse transcription-polymerase chain reaction) primer and a general kit for detecting foot and mouth disease viruses. The nucleotide sequence of an upstream primer of the primer is 5'-CTGGGCCCTTCTTCTTC-3', and the nucleotide sequence of a downstream primer of the primer is 5'-GCTGTTTCTCTGCTCTCTC-3'. The kit comprises the primer. The general RT-PCR primer and the kit which are disclosed by the invention and are used for detecting O-type, A-type and sub-I viruses of foot and mouth diseases have the advantages of high sensitivity, high specificity, quickness, simplicity and the like.

Description

technical field [0001] The invention belongs to the technical field of animal virus molecular biological detection, and in particular relates to a pair of general-purpose RT-PCR primers and a kit for detecting foot-and-mouth disease virus. Background technique [0002] Foot and mouth disease (FMD) is an acute and highly contagious infectious disease caused by foot and mouth disease virus, which mainly affects cloven-hoofed animals, such as cattle, pigs, sheep, etc. It is characterized by fever and vesicular ulceration of the skin or mucous membranes, especially in the mouth and fork. [0003] Foot and mouth disease virus (FMDV) belongs to the picornavirus family and consists of about 8500 nucleotides. The viral genome consists of an open reading frame (ORF), a 5' non-coding region (NCR) and a 3' non-coding region (NCR), and the ORF code consists of four structural proteins (VP1, VP2, VP3 and VP4) , RNA polymerase (3D), protease (L, 2A, 3C) and other non-structural proteins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/70C12Q1/686C12Q2531/113
Inventor 冯淑萍颜健华胡巧云其他发明人请求不公开姓名
Owner 广西壮族自治区动物疫病预防控制中心