Experimental method for preventing generation and growth of liver cancer by using umbilical cord mesenchymal stem cells

A technology of stem cells and experimental methods, applied in the field of preventing the occurrence and growth of liver cancer, can solve the problems of low cure rate and increase the prevalence of liver cancer, and achieve the effects of avoiding side effects, preventing cell clustering, and preventing the occurrence of liver cancer.

Pending Publication Date: 2019-10-22
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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Problems solved by technology

In the present invention, liver cancer is selected as the representative of malignant tumors. Liver cancer is the tumor with the highest cancer incidence rate in my country. Smoking or life stress will increase the prevalence of liver cancer. At present, the only way to treat liver cancer is surgical resection and the cure rate is low.

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  • Experimental method for preventing generation and growth of liver cancer by using umbilical cord mesenchymal stem cells
  • Experimental method for preventing generation and growth of liver cancer by using umbilical cord mesenchymal stem cells
  • Experimental method for preventing generation and growth of liver cancer by using umbilical cord mesenchymal stem cells

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0034] An experimental method for using umbilical cord mesenchymal stem cells to prevent the occurrence and growth of liver cancer, characterized in that: the specific experimental steps are as follows:

[0035] (1) Select 15 NOD / SCID mice aged 4 weeks and weighing 18-22 g, and randomly divide them into normal saline group, UC-MSCs low-dose group and UC-MSCs high-dose group, 5 in each group;

[0036] (2) Select liver cancer cells HepG2. Before the experiment, HepG2 cells need to be transfected with lentivirus to make them with RFP+Luciferse red fluorescent markers. After transfection, use 1-2 μg / mL puromycin to screen until the purity reaches more than 90%. , the stable HepG2 cells were expanded and fr...

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Abstract

The invention relates to an experimental method for preventing generation and growth of liver cancer by using umbilical cord mesenchymal stem cells. For the first time, an application of the umbilicalcord mesenchymal stem cells to prevent the generation and growth of liver cancer is provided. The UC-MSCs cell preparation method is provided, and the experimental data of the dose of 1*104 cells/treatment course and administration method of the UC-MSCs cells for preventing liver cancer are provided. A UC-MSCs preparation is transplanted firstly, then the liver cancer cells are implanted, and thetumor growth of NOD/SCID mice is observed and compared, the method proves that the umbilical cord mesenchymal stem cells can prevent the generation and development of liver cancer and the dosage of UC-MSCs is provided, the self-renewal and damage repair functions of UC-MSCs in the body's adult organ tissue can be maximized, the body's immunity is improved, and the generation of liver cancer can be prevented, which is beneficial to play a more significant and stable role in later research and clinical applications.

Description

technical field [0001] The invention belongs to the fields of cytology and immunology, and relates to stem cell technology, in particular to an experimental method for preventing occurrence and growth of liver cancer by using umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are a type of undifferentiated cells with self-renewal replication and multi-lineage differentiation potential, which play an irreplaceable role in promoting the self-renewal and damage repair of adult organ tissues in organisms. Role. Among various types of stem cells, umbilical cord-derived mesenchymal stem cells (UC-MSCs) exhibit higher cell proliferation activity and differentiation potential, while UC-MSCs have no or low expression and Histocompatibility-related immune molecules are not tumorigenic in animal safety evaluation, so they are receiving more and more attention. The advantages of UC-MSCs also include: taken from ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12Q1/02
CPCC12N5/0665G01N33/5011
Inventor 卢君君许蕊马洁赵刚
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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