Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting lymphoid cancer cell P388D1 from proliferation

A green plum cold tablet and extraction technology, which is applied in the direction of making medicines into special physical or taking forms, drug combinations, anti-tumor drugs, etc., can solve the problems of large dosage, rough process, and many impurities, and achieve the goal of taking medicine Small dose, no bitterness in the dose, and the effect of reducing the dose

Inactive Publication Date: 2014-05-07
李福锦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the use of supercritical and microwave technology in the extraction and preparation of Qingmei Ganmao Granules. However, the method of extracting volatile oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of fenugreek, 200g of dog liver dish, 134g of licorice, 134g of Centella asiatica, 267g of Gangmei, and add Artemisia annua and lavender to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 200min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, crushed, added 5L of 50% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 700W, and the extraction is performed twice. For 8 minutes each time, combine the extracts, concentrate, add to the D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, recover ethanol under reduce...

Embodiment 2

[0033] Take 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of fenugreek, 200g of dog liver dish, 134g of licorice, 134g of Centella asiatica, 267g of Gangmei, and add Artemisia annua and lavender to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 4%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, crushed, added 5L of 50% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 600W, and the extraction is performed twice. Combine the extracts for 5 minutes each time, concentrate, add to the D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, recover ethanol under reduced...

Embodiment 3

[0036] Take 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of fenugreek, 200g of dog liver dish, 134g of licorice, 134g of Centella asiatica, 267g of Gangmei, and add Artemisia annua and lavender to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 6%, the extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 220min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, pulverized, added 5L of 50% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 800W, and the extraction is performed twice. For 10 minutes each time, combine the extracts, concentrate, add to the D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, recover ethanol under re...

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PUM

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly provides a preparation method and application of a Qingmei cold tablet. The preparation method comprises the following steps: by using 200g of southernwood, 267g of finger citron, 134g of elsholtzia, 200g of dicliptera, 134g of licorice, 134g of cat's-foot and 267g of holly root as active pharmaceutical ingredients, carrying out supercritical extraction and microwave extraction so that the glycyrrhizic acid content is greatly enhanced. The invention also provides application of the Qingmei cold tablet in preparing drugs for inhibiting mouse lymphoid cancer cell P388D1 from cell proliferation.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a preparation method of Qingmei Ganmao tablet and its application in medicines for inhibiting the proliferation of mouse lymphoid tumor cell P388D1 cells. Background technique [0002] The standard number of Qingmei Ganmao Granules is WS-11318 (ZD-1318)-2002, which is recorded in the Spleen and Stomach Subvolume of the National Compilation of Chinese Patent Medicine Standards. It is made from 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of Carrot, 200g of Dog Liver, 134g of Licorice, 134g of Centella asiatica, and 267g of Gangmei, which has cooling and antipyretic effects. For Fengreganmao, headache, cough, stuffy nose. [0003] In the prior art, there is no report on the use of supercritical and microwave technology in the extraction and preparation of Qingmei Ganmao Granules. However, the method of extracting volatile oil and d...

Claims

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Application Information

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IPC IPC(8): A61K36/752A61K36/85A61K9/20A61P35/00A61J3/00
Inventor 郝爱华
Owner 李福锦
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