Cosmetic method, composition for skin care, bacterial cells and dried bacterial cells

A technology of using bacteria and bacteria, applied in the directions of drug combination, preparations for skin care, biochemical equipment and methods, etc., can solve the problems of loss of moisturizing ingredients, reaction, killing, etc., to maintain or improve health status with less adverse effects , the effect of inhibiting reproduction

Active Publication Date: 2016-08-24
BIOGENOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if these cleaning agents are used too much, it will not only cause the loss of skin moisturizing ingredients such as ceramide, but also cause adverse effects by killing useful bacteria

Method used

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  • Cosmetic method, composition for skin care, bacterial cells and dried bacterial cells
  • Cosmetic method, composition for skin care, bacterial cells and dried bacterial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Isolation of Staphylococcus epidermidis from skin resident bacteria and separate culture, and production of dried bacteria

[0076] [1] Acquisition of skin resident bacteria

[0077] The following operations are performed while wearing sterile gloves.

[0078] Gently press a polycarbonate cylinder (inner diameter 25mmФ) that has been washed and sterilized by an autoclave on the skin surface of the forehead of the subject, and soak it in sterilized phosphate-buffered saline (PBS) for 20 seconds. A sterile cotton swab will be used to wipe the exposed skin surface inside the cylinder thoroughly. Remove the cylinder, and use a sterilized cotton swab to wipe off the remaining moisture on the skin surface. The water adhering to the inner side of the skin contact surface of the cylinder was also wiped off with a sterilized cotton swab. These sterilized cotton swabs (the part that came into contact with the collector's hand were broken off.) were put into a steril...

Embodiment 2

[0087] Embodiment 2: clinical trial

[0088] 21 women in the age group of 20 to 50 were used as test subjects. On the first day of the test, skin resident bacteria obtained from their own skin (forehead) were isolated and cultured individually by the procedure described in Example 1. , thus obtaining Staphylococcus epidermidis from its own skin resident bacteria. After administration (reinjection) to the test subjects, the number of Staphylococcus epidermidis on the skin surface and the influence on the moisture content of the epidermis were examined.

[0089] The 21 subjects were divided into Group 1 of 13 (in figure 1 and figure 2 Among them, marked as "Beauty Skin Bacteria Reintroduction Group") and the second group with 8 people (in figure 1 and figure 2 , marked as "blank control group") (without telling which group the subjects were assigned to), and distributed basic cosmetics (non-alcoholic lotion (manufactured by HORIN), moisturizing gel (HORIN Manufactured) an...

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Abstract

Provided are: a novel and safe beauty treatment method which can cope with the individual variation of skin environments readily, has little adverse impacts such as adverse side effects, and enables the selective proliferation of a useful bacterium to thereby keep the normal bacterial skin flora healthy; a skin care composition; and a dried bacterium cell which can be used suitably for the production of the skin care composition. A beauty treatment method comprising a step of collecting bacteria inhabiting the surface of the skin of a human body, a step of selectively proliferating at least one useful bacterium that can be proliferated on the surface of the skin of a human body and can improve the health state of the skin among the collected bacteria, and a step of administering a composition containing at least one selected from the group consisting of a cell of the proliferated useful bacterium, a cell component of the useful bacterium and a substance produced by the useful bacterium onto the skin of a human body; and a skin care composition containing a useful bacterium that has been proliferated selectively in the above-mentioned manner and / or a substance produced by the useful bacterium.

Description

technical field [0001] The present invention relates to a cosmetic method that can be applied to everyone regardless of individual differences in skin environment and has almost no side effects, a skin care composition, and bacteria and dried bacteria used in the production of the skin care composition. Background technique [0002] It is known that about dozens of skin resident bacteria live on the surface of human skin. Among the skin resident bacteria, in addition to harmful bacteria such as Staphylococcus aureus, there are also useful bacteria that are useful in maintaining a healthy skin condition, and keeping the resident bacteria in a normal state is important for improving skin. or the state of the skin surface is favorable. For example, Staphylococcus epidermidis (Staphylococcus sepidermidis) plays a very good auxiliary role in maintaining the skin's moisturizing function and isolation function by producing glycerin. It also exerts a great effect on the reproducti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61Q19/00C12N1/00C12N1/20
CPCA61K8/99A61P17/00A61P17/16A61Q5/00A61Q5/02A61Q5/065A61Q5/10A61Q5/12A61Q7/00A61Q19/00C12N1/20
Inventor 本多英俊出来尾格
Owner BIOGENOMICS
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