Cosmetic method, composition for skin care, bacterial cells and dried bacterial cells
A technology of using bacteria and bacteria, applied in the directions of drug combination, preparations for skin care, biochemical equipment and methods, etc., can solve the problems of loss of moisturizing ingredients, reaction, killing, etc., to maintain or improve health status with less adverse effects , the effect of inhibiting reproduction
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Embodiment 1
[0075] Example 1: Isolation of Staphylococcus epidermidis from skin resident bacteria and separate culture, and production of dried bacteria
[0076] [1] Acquisition of skin resident bacteria
[0077] The following operations are performed while wearing sterile gloves.
[0078] Gently press a polycarbonate cylinder (inner diameter 25mmФ) that has been washed and sterilized by an autoclave on the skin surface of the forehead of the subject, and soak it in sterilized phosphate-buffered saline (PBS) for 20 seconds. A sterile cotton swab will be used to wipe the exposed skin surface inside the cylinder thoroughly. Remove the cylinder, and use a sterilized cotton swab to wipe off the remaining moisture on the skin surface. The water adhering to the inner side of the skin contact surface of the cylinder was also wiped off with a sterilized cotton swab. These sterilized cotton swabs (the part that came into contact with the collector's hand were broken off.) were put into a steril...
Embodiment 2
[0087] Embodiment 2: clinical trial
[0088] 21 women in the age group of 20 to 50 were used as test subjects. On the first day of the test, skin resident bacteria obtained from their own skin (forehead) were isolated and cultured individually by the procedure described in Example 1. , thus obtaining Staphylococcus epidermidis from its own skin resident bacteria. After administration (reinjection) to the test subjects, the number of Staphylococcus epidermidis on the skin surface and the influence on the moisture content of the epidermis were examined.
[0089] The 21 subjects were divided into Group 1 of 13 (in figure 1 and figure 2 Among them, marked as "Beauty Skin Bacteria Reintroduction Group") and the second group with 8 people (in figure 1 and figure 2 , marked as "blank control group") (without telling which group the subjects were assigned to), and distributed basic cosmetics (non-alcoholic lotion (manufactured by HORIN), moisturizing gel (HORIN Manufactured) an...
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