A kind of magnetized compound microbial organic water-soluble fertilizer and its preparation method and application
A compound microorganism and fertilizer technology is applied in the field of magnetized compound microorganism organic water-soluble fertilizer and its preparation, and achieves the effects of good crop absorption efficiency, easy absorption and utilization, and regulation of growth and development.
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Embodiment 1
[0042] Example 1 Preparation of magnetized composite microbial organic water-soluble fertilizer (1)
[0043] 1. Preparation of CM bacterial agent:
[0044] ① Preparation of Vesicle-Arbuscular Mycorrhizal (Vesicalar-Arbuscular) and Yeastbacterium (Yeastbacterium):
[0045] Take roots about 4cm long from the root tips of fresh plants, rinse the soil on the roots with water, then treat them with 75% ethanol for 20s, and then use the freshly prepared 0.1% mercury solution (mercury chloride 1g, concentrated hydrochloric acid 2.5mL , 1L of distilled water) sterilize for 5 minutes, rinse with sterile water 5 times. Use a sterile scalpel to cut the root into 3mm thin slices in a sterile petri dish. Use sterile forceps to place the thin slices in a petri dish containing PDA culture medium. Place 3 pieces in each dish. Cultivate in a constant temperature incubator at 25°C and avoid light. It is frequently observed that when the hyphae grow from the tissue block to form a colony of a certain...
Embodiment 2
[0055] Example 2 Preparation of magnetized composite microbial organic water-soluble fertilizer (2)
[0056] 1. Preparation of CM bacterial agent:
[0057] ① Preparation of Vesicle-Arbuscular Mycorrhizal (Vesicalar-Arbuscular) and Yeastbacterium (Yeastbacterium):
[0058] Take roots about 3cm long from the root tips of fresh plants, rinse the soil on the roots with water, then treat them with 75% ethanol for 30s, and then use the freshly prepared 0.1% mercury solution (mercury chloride 1g, concentrated hydrochloric acid 2.5mL , 1L of distilled water) sterilize for 10 minutes, rinse with sterile water 5 times. In a sterile petri dish, use a sterile scalpel to cut the roots into 5mm thin slices. Use sterile forceps to place the thin slices in a petri dish containing PDA culture medium, and place 4 pieces in each dish. Cultivate in a constant temperature incubator at 25°C and avoid light. It is frequently observed that when the hyphae grow from the tissue block to form a colony of a ...
Embodiment 3
[0068] Example 3 Preparation of Magnetized Composite Microbial Organic Water-soluble Fertilizer (3)
[0069] 1. Preparation of CM bacterial agent:
[0070] ① Preparation of Vesicle-Arbuscular Mycorrhizal (Vesicalar-Arbuscular) and Yeastbacterium (Yeastbacterium):
[0071] Take roots about 4cm long from the root tips of fresh plants, rinse the soil on the roots with clean water, then treat them with 75% ethanol for 25s, then use the freshly prepared 0.1% mercury solution (mercury chloride 1g, concentrated hydrochloric acid 2.5mL , 1L of distilled water) sterilize for 8 minutes, rinse with sterile water 5 times. In a sterile petri dish, use a sterile scalpel to cut the root into 4mm thin slices. Use sterile tweezers to place the slices in a petri dish containing PDA culture medium, and place 4 pieces in each dish. Cultivate in a constant temperature incubator at 25°C and avoid light. It is frequently observed that when the hyphae grow from the tissue block to form a colony of a cert...
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