Novel use of substances for detecting nucleotides at rs7597593 and rs1344706
A nucleotide and site technology, applied in the field of biomedicine, can solve the problem of unclear exact function of ZNF804A gene, and achieve the effect of preventing heroin addiction disease and having great application value.
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Embodiment 1
[0030] Example 1. Detect the nucleotides at rs7597593 and rs1344706 in subjects, and obtain the combined genotypes of subjects based on rs7597593 and rs1344706
[0031] 1. Extract the genomic DNA of the subject's peripheral venous blood.
[0032] 2. Using the genomic DNA extracted in step 1 as a template, a specific primer pair composed of rs7597593-upper and rs7597593-lower is used for PCR amplification to obtain PCR amplification products.
[0033] 3. Sequencing the PCR amplified product of step 2 to know the genotype of the subject based on the rs7597593 locus. The sequencing results showed that the PCR amplified products were all target sequences in the ZNF804A gene, and there was no other non-specific amplification, that is, for the specific primer pair, there were no other targets that could be amplified in the human genome. good.
[0034] 4. Using the genomic DNA extracted in step 1 as a template, a specific primer pair composed of rs1344706-upper and rs1344706-lower is used f...
Embodiment 2
[0046] Example 2: Edward Gambling Test (Iowa Gambling Task, IGT)
[0047] Using IGT classic card method, there are four sets of cards (A, B, C, D), each set has 40 cards, stacked according to a certain rule. Among them, Group A and Group B are high-risk cards with higher scores and higher scores. The more cards selected from these two groups, the lower the final total score; Group C and Group D are low-risk cards with low scores. There are fewer points lost, the more you choose between these two sets of cards, the higher the final total score. Without knowing the score of each card in advance, the subject, based on the principle of maximizing his total score, randomly selected one card from the four sets of cards at a time according to the instruction, and selected 100 cards in total to stop. Finally, the total number of low-risk group C and D cards minus the total number of high-risk group A and B cards ((C+D)-(A+B)) represents the subject’s impulsive decision-making ability an...
Embodiment 3
[0053] Example 3. Combined genotype changes in brain imaging of subjects
[0054] In the imaging department of Zhongshan Hospital of Traditional Chinese Medicine, Guangdong Province, a magnetic resonance scanner was used to scan the subject's head. The subject’s head was fixed with the matching foam pad, and the ears were plugged with rubber plugs to reduce noise interference. The subject remained at rest during the entire scanning process, lying on his back quietly, and closing his eyes. A total of 2 different MR sequences (3D-T1; T2) were scanned, and each sequence was repeated. First complete the scanning of the positioning image, and then perform the axial 3D-T1 spoiled gradient echo sequence (SPGR) scan. The scanning parameters are: layer thickness = 1 mm, repetition time (TR) = 7.816 milliseconds, echo time (TE) =2.984 milliseconds, reversal time=450ms, reversal angle=13°, matrix=256×256, field of view (FOV)=256×256mm 2 , The average number of times = 2. During the scanni...
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