New application of substance for detecting nucleotide at rs1344706 site in ZNF804A gene
A nucleotide and site technology, applied in the field of new application of substances used to detect nucleotides at the rs1344706 site in the ZNF804A gene, can solve the problems of unclear exact function of the ZNF804A gene, and achieve the prevention of heroin addiction The effect of disease, significant application value
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Example 1. Detecting the genotype of the subject based on the rs1344706 locus, obtaining the genotype distribution of the subject population and the allele distribution of the subject population
[0027] 1. To detect the genotype of each subject based on the rs1344706 locus, the method is as follows:
[0028] 1. Extract the genomic DNA from the peripheral venous blood of the subjects.
[0029] 2. Using the genomic DNA extracted in step 1 as a template, PCR amplification is performed using a specific primer pair composed of rs1344706-upper-2 and rs1344706-lower-2 to obtain a PCR amplification product.
[0030]3. Sequencing the PCR amplification product in step 2, so as to know the genotype of the subject based on the rs1344706 site. The sequencing results showed that the PCR amplification products were all the target sequences in the ZNF804A gene, and there was no other non-specific amplification, that is, for the specific primer pair, there were no other amplified targ...
Embodiment 2
[0039] Embodiment 2, the sensitivity detection of primer pair
[0040] Randomly select 5 persons from the group of heroin addicts, and 5 persons are randomly selected from the group of normal subjects.
[0041] 1. Sensitivity of detecting primers with different contents to amplify a fixed amount of template DNA
[0042] 1. Extract the genomic DNA from the peripheral venous blood of the subjects.
[0043] 2. Using the genomic DNA extracted in step 1 as a template, use the primer pair composed of rs1344706-upper-1 and rs1344706-lower-1 to carry out PCR amplification, and then perform agarose gel electrophoresis on the PCR amplification product. The results are shown in figure 2 a.
[0044] PCR system (10μl): 10×PCR Buffer (containing 20mM MgCl 2 ) 1μl, MgCl 2 Aqueous solution (25mM) 0.8μl, dNTP mix (25mM each) 0.2μl, primer mix 1.0μl (containing equimolar mixture of rs1344706-upper-1 and rs1344706-lower-1), Hotstar Tag (5U / μl) 0.2μl, template DNA 1 μl, with H 2 O to make ...
PUM
Property | Measurement | Unit |
---|---|---|
Sensitivity | aaaaa | aaaaa |
Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com