New application of substance for detecting nucleotide at rs1344706 site in ZNF804A gene

A nucleotide and site technology, applied in the field of new application of substances used to detect nucleotides at the rs1344706 site in the ZNF804A gene, can solve the problems of unclear exact function of the ZNF804A gene, and achieve the prevention of heroin addiction The effect of disease, significant application value

Active Publication Date: 2014-05-07
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the exact function of the ZNF804A gene is unknown

Method used

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  • New application of substance for detecting nucleotide at rs1344706 site in ZNF804A gene
  • New application of substance for detecting nucleotide at rs1344706 site in ZNF804A gene
  • New application of substance for detecting nucleotide at rs1344706 site in ZNF804A gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Detecting the genotype of the subject based on the rs1344706 locus, obtaining the genotype distribution of the subject population and the allele distribution of the subject population

[0027] 1. To detect the genotype of each subject based on the rs1344706 locus, the method is as follows:

[0028] 1. Extract the genomic DNA from the peripheral venous blood of the subjects.

[0029] 2. Using the genomic DNA extracted in step 1 as a template, PCR amplification is performed using a specific primer pair composed of rs1344706-upper-2 and rs1344706-lower-2 to obtain a PCR amplification product.

[0030]3. Sequencing the PCR amplification product in step 2, so as to know the genotype of the subject based on the rs1344706 site. The sequencing results showed that the PCR amplification products were all the target sequences in the ZNF804A gene, and there was no other non-specific amplification, that is, for the specific primer pair, there were no other amplified targ...

Embodiment 2

[0039] Embodiment 2, the sensitivity detection of primer pair

[0040] Randomly select 5 persons from the group of heroin addicts, and 5 persons are randomly selected from the group of normal subjects.

[0041] 1. Sensitivity of detecting primers with different contents to amplify a fixed amount of template DNA

[0042] 1. Extract the genomic DNA from the peripheral venous blood of the subjects.

[0043] 2. Using the genomic DNA extracted in step 1 as a template, use the primer pair composed of rs1344706-upper-1 and rs1344706-lower-1 to carry out PCR amplification, and then perform agarose gel electrophoresis on the PCR amplification product. The results are shown in figure 2 a.

[0044] PCR system (10μl): 10×PCR Buffer (containing 20mM MgCl 2 ) 1μl, MgCl 2 Aqueous solution (25mM) 0.8μl, dNTP mix (25mM each) 0.2μl, primer mix 1.0μl (containing equimolar mixture of rs1344706-upper-1 and rs1344706-lower-1), Hotstar Tag (5U / μl) 0.2μl, template DNA 1 μl, with H 2 O to make ...

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Abstract

The invention discloses a new application of a substance for detecting nucleotide at the rs1344706 site in a ZNF804A gene, and particularly relates to a new application of the substance for detecting nucleotide at the rs1344706 site in preparing a kit for evaluating the risk of heroin addiction and / or the damage degree of the impulsive decision-making behavior after heroin addiction. The new application is conductive to preventing heroin addiction diseases, facilitating carrying out effective intervention on heroin addicts and developing new medicines for the heroin addicts and has great application value.

Description

technical field [0001] The present invention relates to a new application of the substance used to detect the nucleotide at the rs1344706 site in the ZNF804A gene, specifically, the substance used to detect the nucleotide at the rs1344706 site is used in the preparation of the substance used to assess the risk of heroin addiction and Novel uses in the kit for the degree of impairment of impulsive decision-making behavior after heroin addiction and / or heroin addiction. Background technique [0002] Substance addiction, also known as substance dependence, refers to a strong and irresistible state of looking for the substance psychologically and physically after long-term abuse of a substance, in order to experience the euphoria of repeated use of the substance. At the same time avoid physical discomfort caused by withdrawal. Substance addiction disorder is a chronic recurrent encephalopathy characterized by recurrent attacks of compulsive drug-seeking and drug-taking behavior...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 时杰孙艳王贵彬陆林朱维莉赵励彦
Owner PEKING UNIV
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