Polyacrylamide gel solution for microsatellite marker polymorphism detection and silver staining method
A technology of polyacrylamide gel and polyacrylamide gel mother, which is applied in the biological field, can solve the problems of PAG gel being easily broken, the bands are difficult to distinguish, and the error of the result is increased, so as to achieve superior detection sensitivity, easy operation, Effects in simple steps
Inactive Publication Date: 2014-08-27
TARIM UNIV
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At this concentration, PAG is completely transparent and elastic, but in the process of color development, PAG glue is fragile, and it needs to be "puzzled" when taking pictures. In data analysis, the spliced PAG after fra
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The invention discloses a polyacrylamide gel (PAG) solution for microsatellite marker (also known as simple sequence repeats or SSR) polymorphism detection. The polyacrylamide gel solution includes a polyacrylamide gel mother liquor, 5*TBE, a 10% APS and TEMED, diluting is carried out by adopting distilled water until the volume of the polyacrylamide gel mother liquor accounts for 21-22% of the total volume, and the amount of the 5*TBE is the same as that of the polyacrylamide gel mother liquor. When the polyacrylamide gel solution is used, PAG has less damage during an oscillation developing process, PAG splicing is avoided, the experimental error is reduced, and the accuracy of results is guaranteed.
Description
technical field [0001] The invention relates to a gel reagent used in an SSR detection process and a method for using the same, belonging to the field of biotechnology. Background technique [0002] Microsatellite (SSR), also known as Simple Sequence Repeats (SSR), is a piece of DNA composed of basic units consisting of 1 to 6 nucleotides repeated multiple times, widely distributed in different positions of the genome, and the length is generally between Below 200bp. Microsatellite markers are widely used due to the high inter-individual variability and abundance of repeat units. Microsatellite loci are usually amplified by PCR, and the amplified products are analyzed by electrophoresis and alleles are separated by size for detection. [0003] Among the SSR polymorphism detection methods, PAGE polyacrylamide gel electrophoresis (polyacrylamide gel electrophoresis) is a widely used method, and the polyacrylamide gel electrophoresis is stained by silver staining. [0004] T...
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Inventor 聂竹兰魏杰王帅马振华程勇张大龙张玲任道泉宋勇罗伟王卫民王智超陈生熬雷曼红
Owner TARIM UNIV
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