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Kit for predicting leukocyte antigen genotypes of the Han people by using single-nucleotide polymorphisms

A single nucleotide polytype, antigen gene technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem that the model cannot use leukocyte antigen genotype, and the distribution of human leukocyte tissue antigen gene categories is different. And other issues

Inactive Publication Date: 2014-10-22
ACAD SINIC +2
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Problems solved by technology

However, most of the HLA genotype prediction models only include Caucasian samples, and few studies have reported non-Caucasian samples, and the distribution of HLA gene types varies among different races
[0004] Zheng et al. emphasized in the BMC genetics journal in 2011 that after constructing the prediction model of leukocyte antigen, the model cannot be used for leukocyte antigen genotypes of different races
Therefore, Ayele et al. published in 2011 in the PLOS ONE journal that they have constructed a prediction model for Africans-specific leukocyte antigen; however, there is no prediction model for Asian human leukocyte antigen.

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  • Kit for predicting leukocyte antigen genotypes of the Han people by using single-nucleotide polymorphisms
  • Kit for predicting leukocyte antigen genotypes of the Han people by using single-nucleotide polymorphisms
  • Kit for predicting leukocyte antigen genotypes of the Han people by using single-nucleotide polymorphisms

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Embodiment 1

[0095] Utilize above-mentioned result, can develop a kind of single nucleotide polymorphism accordingly in the application in preparation prediction human leukocyte antigen allele kit, its steps include:

[0096] (a) provide a human nucleic acid sample;

[0097] (b) identifying the genotype of a set of single nucleotide polymorphisms of the human nucleic acid sample, the set of single nucleotide polymorphisms comprising each of the different single nucleotide polymorphisms located on the human leukocyte antigen gene;

[0098] (c) analyzing the genotype of each SNP in step (b) using a predictive model to obtain a calculated value, wherein the predictive model uses the genotype of the SNP to predict human leukocyte antigen alleles; and

[0099] (d) predicting the HLA allotype of the human sample according to the calculated value obtained in step (c).

[0100] The present invention uses the specific single nucleotide polymorphism of the Han population and predicts the genotypes...

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Abstract

The invention discloses a kit for predicting leukocyte antigen genotypes of the Han people by using single-nucleotide polymorphisms and applications thereof. Models are predicted for the human leukocyte antigen genotypes (HLA-A, HLA-B, HLA-C, HLA-DPB1, HLA-DQB1 and HLA-DRB1) of the Asians, and the prediction results achieve optimization of the models. The typical human leukocyte antigen alleles types of the Asians are predicted according to the fixed genotypes of the single-nucleotide polymorphisms with a high accuracy ranging from 80.37% (HLA-B) to 95.79% (HLA-DQB1). Besides, the kit for predicting the leukocyte antigen alleles types by using the genotypes can save a considerable amount of time and cost.

Description

technical field [0001] The present invention relates to a kit for predicting human leukocyte antigen alleles with single nucleotide polymorphisms with ethnic specificity, in particular, the present invention relates to using single nucleotide polymorphisms of Han Chinese to predict human leukocyte antigens Antigen Genotyping Kit. Background technique [0002] The human leukocyte tissue antigen gene group is located on the sixth pair of chromosomes and is divided into major histocompatibility complex class I (HLA-A, HLA-B, and HLA-C) and major histocompatibility complex class II (HLA -DR, HLA-DQ, and HLA-DP) alleles, and the polymorphism (polymorphism) pattern of multiple alleles of a single gene in an individual causes graft rejection (graft) during tissue or organ transplantation rejection) and graft-versus-host diseases. HLA alleles also play important roles in population genetics and immune-related disease status. Furthermore, previous comparative studies have shown th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6881C12Q2600/156
Inventor 范盛娟张天钧杨伟勋陈沛隆谢瑷如陈垣崇朱正中
Owner ACAD SINIC
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