Warburgia ugandensis tissue culture rapid propagation method

A tissue culture, dozens of camphor technology, applied in the field of plant tissue culture, to achieve the effect of speeding up the development process

Active Publication Date: 2014-11-19
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieval, up to now, there is no report specifically aimed at the tissue culture of tens of Cinnamomum camphora in the prior art

Method used

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  • Warburgia ugandensis tissue culture rapid propagation method
  • Warburgia ugandensis tissue culture rapid propagation method
  • Warburgia ugandensis tissue culture rapid propagation method

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Experimental program
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Embodiment Construction

[0038] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0039] 1. Method

[0040] ① Disinfection of explants

[0041] The explant used for callus induction is the young leaf base or shoot base near the petiole;

[0042] Many experiments have proved that the healing rate of this part is high, while other parts of the leaves (including unexpanded young leaves) can callus at the wound, but the healing rate is low.

[0043] The explants were sterilized with 0.1% mercury liter for 6 minutes, then discarded the waste liquid, and then sterilized with new 0.1% mercury liter for 6 minutes, then discarded the waste liquid, and then rinsed with sterile water for 6 to 8 times;

[0044] ②Callus induction

[0045] The medium used for callus induction is: MS + 1.6mg / L 6BA + 1mg / L IBA + 0.1mg / L TDZ; the explants can be seen to expand within 1 week after inoculation, and gradually form a yellow-green callus (local There are dark brown)

[0046] See fig...

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Abstract

The present invention discloses a Warburgia ugandensis tissue culture rapid propagation method, and relates to a plant tissue culture technology in molecular biology experiments. The method comprises: 1, disinfecting explants, 2, carrying out callus induction, 4, elongating seedlings, 5, inducing roots, and 6, carrying out seedling exercising and transplantation. According to the present invention, a lot of young seedlings of the Warburgia ugandensis imported from Africa can be obtained through tissue culture rapid propagation so as to provide sufficient plant materials for extraction of the active pharmaceutical ingredient of the Warburgia ugandensis in the downstream and accelerate the development process of oral health care products.

Description

technical field [0001] The invention relates to plant tissue culture techniques in molecular biology experiments, in particular to a method for rapid propagation of tens of camphor tissue culture. Background technique [0002] Dozens of camphor ( Warburgia ugandensis ) is a medicinal plant endemic to Africa. The branches of this plant are used as "tooth sticks" in Africa and have broad-spectrum antibacterial activity. The natural heat plant-based antibacterial agent extracted from Shida camphor will help to develop a series of health care products, especially health toothpaste, which can be used for oral health care in the future. The present invention establishes a ten-odd camphor tissue culture regeneration system, which can provide sufficient plant materials for downstream extraction and analysis of medicinal active ingredients. After retrieval, up to now, there is no report specifically aimed at the tissue culture of Ten Da Cinnamomum camphora in the prior art. Conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吕世友张玲玲
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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