Warburgia ugandensis tissue culture rapid propagation method
A tissue culture, dozens of camphor technology, applied in the field of plant tissue culture, to achieve the effect of speeding up the development process
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[0038] Below in conjunction with accompanying drawing and embodiment describe in detail:
[0039] 1. Method
[0040] ① Disinfection of explants
[0041] The explant used for callus induction is the young leaf base or shoot base near the petiole;
[0042] Many experiments have proved that the healing rate of this part is high, while other parts of the leaves (including unexpanded young leaves) can callus at the wound, but the healing rate is low.
[0043] The explants were sterilized with 0.1% mercury liter for 6 minutes, then discarded the waste liquid, and then sterilized with new 0.1% mercury liter for 6 minutes, then discarded the waste liquid, and then rinsed with sterile water for 6 to 8 times;
[0044] ②Callus induction
[0045] The medium used for callus induction is: MS + 1.6mg / L 6BA + 1mg / L IBA + 0.1mg / L TDZ; the explants can be seen to expand within 1 week after inoculation, and gradually form a yellow-green callus (local There are dark brown)
[0046] See fig...
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