Method for detecting vomitoxin and zearalenone in grains and feeds

Abstract

The invention discloses a method for detecting vomitoxin and zearalenone in grains and feeds. The method comprises the following steps: A, adding 25g of a to-be-detected sample into a 250ml conical flask; B, adding 100ml of an extracting solution into the conical flask, and shaking the conical flask on a shaker for 20 minutes to obtain a mixed solution; C, adding 8ml of the mixed solution into a glass test tube, adding 80mul of acetic acid into the test tube, and performing column purification by virtue of a multifunctional purification column to obtain a purification solution; D, adding 250 microlitres of the purification solution into a container, adding deionized water containing 0.05 percent of acetic acid into the container for making up to volume to 1ml, thereby obtaining a test sample; and E, extracting the solution from the test sample, detecting by utilizing a multi-reaction detection mode of a liquid chromatography-tandem mass spectrometer, thereby obtaining data of the vomitoxin and the zearalenone respectively. According to the method, the operation steps have the time / labor-saving effect, the production and processing cost is reduced, and the detection result is relatively accurate and sensitive.

Description

technical field [0001] The invention relates to the application field of a method for measuring deoxynivalenol and zearalenone, in particular to a method for detecting deoxynivalenol and zearalenone in grain and feed. Background technique [0002] At present, the methods for determining the content of deoxynivalenol and zearalenone in grain and feed often need to be determined separately, and both require very cumbersome steps, and need to use a large number of auxiliary solution reagents and solid samples, which not only makes the operation The steps become time-consuming and laborious, and the cost of production and processing is increased due to the separate determination and addition of a large number of reagents and samples. At the same time, the determination method also has defects such as inaccurate and insensitive determination results. Contents of the invention [0003] In order to solve the problems in the above-mentioned background technology, the object of the...

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Problems solved by technology

[0002] At present, the methods for determining the content of deoxynivalenol and zearalenone in grain and feed often need to be determined separately, and both require very cumbersome steps, and need to use a large number of auxiliary solution reagents and solid samples, which not only makes the operation The steps become time-consuming and laborious, and the cost of production and processing is increased due to the separate measurement and addition of a large number of reagents and samples. At the same time, this measurement method also has defects such as inaccurate and insensitive measurement results.