Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular specific marker primers for sakura varieties including Superba and Grandifora

A marker primer, specific technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the difficulty in identifying phenotypic characteristics, the application and promotion of garden plants and the breeding of new varieties and other problems, to achieve the effect of a simple method

Inactive Publication Date: 2015-05-13
CHENGBANG ECO ENVIRONMENT CO LTD
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After long-term investigation work, we found that 20 late cherry varieties include Hongli (Cerasus serrulata'Benigasa'), Honghua (C.serrulata'Kouka'), Yiye (C.serrulata'Hisakura'), Fulushou ( C.serrulata'Contorta'), Samantabhadra (C.serrulata'Albo-rosea'), Songyue (C.serrulata'Superba'), Yujin (C.serrulata'Grandifora'), Hongfeng (Cerasus×sieboldii' Beni‐yutaka'), Royal Clothes Yellow (C.serrulata'Gioiko'), Guanshan (C.serrulata'Kanzan'), Lantern (C.serrulata'Ojochin'), Eightfold Red Branch Weeping (C.spachiana'Plena Rosea' ), Ichihara Huwei (C.serrulata'Albo Plena'), Sakuyahime (Cerasus×yedoensis'Sakuyahime'), Suzaku (C.serrulata'Shujaku'), Yang Guifei (C.serrulata'Mollis'), Yuqing Zhiwei (C.spachiana'Ujou‐shidare'), Chrysanthemum weeping cherry (C.serrulata'Plena-pendula'), Hirano sister back (C.serrulata'Imose'), Asahiyama cherry (C.serrulata'Asahiyama') in the garden It is widely used, but the phenomenon of "same names and different objects" and "same objects and different names" of these varieties is also very serious, and it is difficult to identify them from the aspect of phenotypic characteristics, which brings many difficulties to the application, promotion and selection of new varieties of garden plants. Therefore, Focusing on the development of stable and specific DNA fingerprints for these varieties at the molecular level is the scientific way to achieve accurate and rapid identification of cherry blossom varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular specific marker primers for sakura varieties including Superba and Grandifora
  • Molecular specific marker primers for sakura varieties including Superba and Grandifora
  • Molecular specific marker primers for sakura varieties including Superba and Grandifora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Extraction of Genomic DNA of Cherry Blossom Varieties:

[0034] Take 0.01g of the young leaves of the cherry blossom variety to be tested, add liquid nitrogen and grind them thoroughly, and use the SDS-CTAB method to extract the genomic DNA. After multiple extractions, the crude genomic DNA extract of the cherry blossom variety is obtained. The crude DNA was purified by Magabio Nucleic Acid Purification Kit (Bioer, Hangzhou, China), and detected by 1.5% agarose gel electrophoresis and DNA / RNA UV spectrophotometer (GeneQuant Pro, GE Healthcare). Integrity, Purity and Concentration. DNA samples with OD260 / OD280>1.8 were used for subsequent PCR amplification. DNA extracts were stored in a -20°C refrigerator for later use.

[0035] (2) Design specific PCR amplification primers, the sequence of the primer pair is:

[0036] The upstream primer: 5'-AGCGGCCGCACAAATTAAGAAAAA-3' and the downstream primer: 5'-AGCGGCCGCACTTTGAACGAT-3' were synthesized by Shanghai Bioengineer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a pair of high-specificity molecular specific marker primers for sakura varieties including Superba and Grandifora, and a method for quickly identifying the sakura varieties including Superba and Grandifora. Primer sequences are as follows: an upstream primer: 5'-AGCGGCCGCACAAATTAAGAAAAA-3'; a downstream primer: 5'-AGCGGCCGCACTTTGAACGAT-3'. The molecular specific marker primers can be used for quick preliminary identification of the sakura varieties including Superba and Grandifora, and the method is simple, quick and convenient and is a molecular means irreplaceable by apparent characteristic identification of the sakura varieties.

Description

(1) Technical field [0001] The invention relates to molecular specific marker primers of cherry blossom varieties "Songyue" and "Yujin" and a method for quickly identifying the cherry blossom varieties "Songyue" and "Yujin" by using the primers. (2) Background technology [0002] Cherry blossoms belong to the genus Cerasus of the Rosaceae (Rosaceae) and are world-renowned ornamental plants. At present, there are more than 300 known varieties of cherry blossoms, which have great application value in gardens because of their beautiful plant shape, bright colors and long flowering period. In recent years, cherry blossoms have been planted on a large scale in various parts of my country and used in parks, schools, streets, courtyards and other green spaces, becoming one of the main ornamental flowers and trees in early spring. Beijing, Wuhan, Qingdao, Shanghai and other cities hold cherry blossom festivals every year, and the cherry blossom industry has developed rapidly. This ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 徐梁李海波杨少宗魏海龙赵庆杰柳新红
Owner CHENGBANG ECO ENVIRONMENT CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com