Molecular specific marker primers for No. 4 and No.32 of an improved variety Changlin of Camellia oleifera and an identification method

Abstract

The present invention provides molecular specific marker primers for No. 4 and No.32 of an improved variety Changlin of Camellia oleifera. The sequences of the primers are: an upstream primer: 5'-CCTATGGTGCCTCTTTGTTTGATGT-3'; and downstream primer: 5'-GTGACAAGTTGAACTCTAGCACAAA-3'. By employing the molecular specific marker primers of the present invention, quick identification of the No. 4 and No.32 of the improved variety Changlin of Camellia oleifera in early stage can be achieved; the method is simple, fast and accurate, so it is a molecular method that can not be substituted by using apparent characteristics to distinguish improved varieties of Camellia oleifera.

Description

(1) Technical field [0001] The invention relates to molecular-specific marker primers for Camellia oleifera varieties Changlin 4 and Changlin 32, and a method for quickly identifying Camellia oleifera varieties Changlin 4 and Changlin 32 using the primers. (2) Background technology [0002] Camellia oleifera is the woody oil tree species with the largest planting area and wide distribution in my country. Vigorously developing the Camellia oleifera industry is of great significance to ensuring the safety of grain and oil, increasing farmers' income, promoting the comprehensive development of mountainous areas and building a new socialist countryside. Camellia oleifera industry in my country is in the ascendant with huge development potential. At present, the total area of ​​camellia oleifera forest in my country is more than 45 million mu, but the output is very low, only about 4 kilograms of camellia oil per mu. The root cause is that most varieties of camellia oleifera ar...

AI Technical Summary

Problems solved by technology

[0004] The identification of camellia oleifera varieties is mainly based on appearance characteristics, but due to the long period of identification of many morphological characters, the great influence of the environment, and the increasing number of varieties, the identification of varieties is becoming more and more difficult

Since this century, some PCR-based molecular marker technologies such as RAPD (Random Amplified Polymorphic DNA, random amplified polymorphic DNA), ISSR (Inter-Simple Sequence Repeat, simple sequence repeat interval amplified polymorphism) and SRAP ( sequence-related amplified polymorphism) have been successively used to study the genetic diversity and genetic distance of Camellia oleifera germplasm resources, but there are few studies on the linkage between molecular markers and Camellia oleifera traits, and the molecular identification between Camellia oleifera varieties. Moreover, these molecular markers are either universal random primers or a random combination of designed primers, the PCR amplification pattern is not only complex, poor repeatability, but also low specificity, so it is not suitable for variety identification

SCAR (Sequence Characterized Amplified Region) marker was proposed by Paran and Michelmore on the basis of RAPD in 1993. It is based on the sequencing of specific RAPD fragments, and a pair of 18-24 bases is designed according to the sequences at both ends The primers are used for specific amplification at a higher annealing temperature. The use of specific primers eliminates the competition between random primer binding sites, so it is a very stable molecular marker. It is widely used in applications. It has the characteristics of rapidity, simplicity and low cost. So far, there has been no research report on the application of SCAR markers in the identification of Camellia oleifera varieties

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