8 results about "GeneQuant" patented technology

The invention belongs to the technical field of analytical chemistry detection, and particularly relates to a construction method and application of a tobacco mosaic virus molecular imprinting sensor based on MIL-101 (Fe). The construction method comprises the following steps: by taking a tobacco mosaic virus TMV as a template virus, preparing a molecularly imprinted polymer MIPs with a TMV specific recognition cavity on the surface of MIL-101 (Fe) through a free radical polymerization reaction; the template virus is removed in the preparation process of the molecularly imprinted polymer MIPs, and a TMV specific recognition cavity of the molecularly imprinted polymer MIPs contains MIL-101 (Fe); the molecularly imprinted polymer MIPs is the tobacco mosaic virus molecularly imprinted sensor based on MIL-101 (Fe). Hydrochloric acid is introduced, so that the TMV specific recognition cavity structure of the molecularly imprinted polymer MIPs is cracked to release Fe < 3 + >, then potassium thiocyanate is added, and the Fe < 3 + > and KSCN are subjected to a complexation reaction to generate a red complex. And the complexation reaction of Fe < 3 + > and KSCN is used as a signal probe, the rapid semi-quantitative visual detection of the target virus TMV by naked eyes is realized, and quantitative detection and analysis are carried out through an ultraviolet spectrophotometer.

The present application relates to the field of probiotic activity detection, in particular to a reagent device for rapid detection of probiotic activity and equipment, comprising a shaking cup and a shaking device; the reagent device further comprises: a separation ring coaxially arranged in the shaking cup along the axis of the shaking cup, the diameter of the separation ring is the same as the diameter of the shaking cup, and the separation ring is made of a deformable material; a sampling device arranged at the upper part of the shaking cup; a storage cup arranged in a straight line with the shaking cup, and a plurality of storage cups are used to store reagents and probiotic samples respectively; a first linear driver for driving the sampling device to reciprocate along the arrangement direction of the storage cup; and an ultraviolet spectrophotometer arranged on one side of the storage cup and located on the moving path of the sampling device. The present application avoids the scattering and shielding interference of foam on the light path, and improves the reliability of the data.

The invention belongs to the technical field of enzyme activity determination. The combined determination method comprises the following steps: (1) adding plant tissues into liquid nitrogen, grinding, adding a phosphate buffer solution with the pH value of 7.8, continuously grinding until no crude fiber exists, and centrifuging to obtain an enzyme extracting solution; (2) determining the activity of SOD in the enzyme extracting solution by adopting a nitrogen blue tetrazole photochemical reduction method; (3) preparing a reaction system by using a phosphate buffer solution with the concentration of 50mmol. L <-1 > and the pH value of 5.5, and determining the POD activity in the enzyme extracting solution by adopting a guaiacol colorimetric method; and (4) determining by adopting an ultraviolet spectrophotometer method, immediately starting timing after H2O2 is added, reading once every 1min until the OD value is unchanged, and calculating the CAT activity by using the average value of OD change per minute. The method can solve the problems of tedious experimental steps, low efficiency, unstable data and the like due to respective extraction of three enzymes, ensures the accuracy of enzyme activity determination, simplifies the experimental steps, and improves the experimental efficiency and the data stability.

The invention provides a method for rapidly screening black fungus strains with high melanin yield and application of the black fungus strains, and belongs to the technical field of biology. According to the method disclosed by the invention, the black fungus fermentation liquid is simply treated, and the ultraviolet spectrophotometer is combined, so that the purpose of quickly and accurately screening the black fungus strains with high melanin yield is achieved, and the workload of screening the black fungus strains is effectively reduced. The invention further provides application of the method in black fungus strain breeding and melanin production with high melanin yield, and improvement of scientific research efficiency and development of the melanin industry are facilitated.

A method for effectively measuring the tear protein content of a soft hydrophilic corneal contact lens is provided in the present invention. The method comprises: preparing a protein extraction solution by mixing acetonitrile, pure water and trifluoroacetic acid; placing the soft hydrophilic corneal contact lens with protein adsorbed on its surface into the protein extraction solution so that the protein extraction solution separates the protein from the soft hydrophilic corneal contact lens; taking out the soft hydrophilic corneal contact lens from the protein extraction solution; detecting a protein concentration in the protein extraction solution by a micro ultraviolet spectrophotometer to obtain a concentration value of the adsorbed protein on the soft hydrophilic corneal contact lens; and calculating a content value of the adsorbed protein on the soft hydrophilic corneal contact lens based on the concentration value of the adsorbed protein on the soft hydrophilic corneal contact lens and a volume of the protein extraction solution. By means of the method, the tear protein content on the surface of the soft hydrophilic corneal contact lens may be quantitatively and qualitatively detected, thus providing users with experimental test data and ensuring the safety of user consumption and use.

The invention belongs to the field of detection and analysis, and relates to a method for measuring protein, starch, cellulose, hemicellulose, pectin and lignin in a sample, which comprises the following steps: extracting the sample with an ethanol saturated salt solution to obtain a liquid phase substance; extracting the solid-phase substance with a dimethyl sulfoxide solution to obtain extract liquor and residues; measuring a liquid phase substance and the extract liquor by a coomassie brilliant blue method to obtain the protein content; carrying out acidolysis on the extract and dilute sulphuric acid, and measuring an acidolysis product by ion chromatography to obtain the content of starch; carrying out acidolysis on the residues and concentrated sulfuric acid, diluting, and continuously carrying out acidolysis to obtain liquid and solid products; measuring the liquid product by ion chromatography to obtain the contents of cellulose, hemicellulose and pectin; measuring the absorbance of a liquid product by using an ultraviolet spectrophotometer to obtain the content of the acid-soluble lignin; drying and roasting the solid product, and changing the mass before and after roasting to obtain the content of the acid-insoluble lignin; the sum of the acid-soluble lignin and the acid-insoluble lignin is the lignin content. The invention also relates to a method and a device for predicting the components in a sample. The method can quickly and accurately determine the contents of the components in the sample, and is high in precision.

The invention relates to the technical field of intelligent agriculture and plant protection, and particularly discloses a broccoli black spot early diagnosis system and method based on multi-mode machine learning fusion, and the system comprises a portable collection platform which is integrated with a hyperspectral imaging camera, a chlorophyll fluorescence imager and a dark adaptation leaf clamp, the platform is provided with a height-adjustable bracket and a controllable light source, so that the consistency of data acquisition conditions is ensured; a physical and chemical index rapid detection module is arranged in the mobile experiment terminal, and the mobile experiment terminal comprises a portable PCR instrument for rapid extraction and amplification of nucleic acid on site and a miniature ultraviolet spectrophotometer for rapid determination of pigment content. According to the broccoli black spot early diagnosis system and method based on multi-modal machine learning fusion, the system is highly integrated, portable and automatic, is suitable for a field environment, and provides an efficient disease diagnosis tool for non-expert users.

This invention belongs to the field of biochemical detection technology and discloses a method for detecting the content of the biomarker GSH based on TMB colorimetric method. The specific technical solution is as follows: Step 1: Hydrogen-bonded organic framework is synthesized by hydrothermal method, and the transition metal Fe is introduced for modification to obtain HOF-Fe material; Step 2: Acetic acid-sodium acetate solution is used as background solution, TMB solution and H2O2 solution are added respectively, and finally HOF-Fe is added to react. The peroxidase-like activity of the material is measured by ultraviolet spectrophotometer; Step 3: Glutathione solution of different concentrations is added to the above reaction system to quantitatively detect glutathione. This method constructs a high-performance colorimetric sensor by preparing a novel HOF-Fe nanozyme, which can realize rapid, high-sensitivity and high-specificity detection of glutathione, and can be widely used in the determination of glutathione content in actual samples.