4 results about "GeneQuant" patented technology

The present application relates to the field of probiotic activity detection, in particular to a reagent device for rapid detection of probiotic activity and equipment, comprising a shaking cup and a shaking device; the reagent device further comprises: a separation ring coaxially arranged in the shaking cup along the axis of the shaking cup, the diameter of the separation ring is the same as the diameter of the shaking cup, and the separation ring is made of a deformable material; a sampling device arranged at the upper part of the shaking cup; a storage cup arranged in a straight line with the shaking cup, and a plurality of storage cups are used to store reagents and probiotic samples respectively; a first linear driver for driving the sampling device to reciprocate along the arrangement direction of the storage cup; and an ultraviolet spectrophotometer arranged on one side of the storage cup and located on the moving path of the sampling device. The present application avoids the scattering and shielding interference of foam on the light path, and improves the reliability of the data.

A method for effectively measuring the tear protein content of a soft hydrophilic corneal contact lens is provided in the present invention. The method comprises: preparing a protein extraction solution by mixing acetonitrile, pure water and trifluoroacetic acid; placing the soft hydrophilic corneal contact lens with protein adsorbed on its surface into the protein extraction solution so that the protein extraction solution separates the protein from the soft hydrophilic corneal contact lens; taking out the soft hydrophilic corneal contact lens from the protein extraction solution; detecting a protein concentration in the protein extraction solution by a micro ultraviolet spectrophotometer to obtain a concentration value of the adsorbed protein on the soft hydrophilic corneal contact lens; and calculating a content value of the adsorbed protein on the soft hydrophilic corneal contact lens based on the concentration value of the adsorbed protein on the soft hydrophilic corneal contact lens and a volume of the protein extraction solution. By means of the method, the tear protein content on the surface of the soft hydrophilic corneal contact lens may be quantitatively and qualitatively detected, thus providing users with experimental test data and ensuring the safety of user consumption and use.

The invention belongs to the field of detection and analysis, and relates to a method for measuring protein, starch, cellulose, hemicellulose, pectin and lignin in a sample, which comprises the following steps: extracting the sample with an ethanol saturated salt solution to obtain a liquid phase substance; extracting the solid-phase substance with a dimethyl sulfoxide solution to obtain extract liquor and residues; measuring a liquid phase substance and the extract liquor by a coomassie brilliant blue method to obtain the protein content; carrying out acidolysis on the extract and dilute sulphuric acid, and measuring an acidolysis product by ion chromatography to obtain the content of starch; carrying out acidolysis on the residues and concentrated sulfuric acid, diluting, and continuously carrying out acidolysis to obtain liquid and solid products; measuring the liquid product by ion chromatography to obtain the contents of cellulose, hemicellulose and pectin; measuring the absorbance of a liquid product by using an ultraviolet spectrophotometer to obtain the content of the acid-soluble lignin; drying and roasting the solid product, and changing the mass before and after roasting to obtain the content of the acid-insoluble lignin; the sum of the acid-soluble lignin and the acid-insoluble lignin is the lignin content. The invention also relates to a method and a device for predicting the components in a sample. The method can quickly and accurately determine the contents of the components in the sample, and is high in precision.

This invention belongs to the field of biochemical detection technology and discloses a method for detecting the content of the biomarker GSH based on TMB colorimetric method. The specific technical solution is as follows: Step 1: Hydrogen-bonded organic framework is synthesized by hydrothermal method, and the transition metal Fe is introduced for modification to obtain HOF-Fe material; Step 2: Acetic acid-sodium acetate solution is used as background solution, TMB solution and H2O2 solution are added respectively, and finally HOF-Fe is added to react. The peroxidase-like activity of the material is measured by ultraviolet spectrophotometer; Step 3: Glutathione solution of different concentrations is added to the above reaction system to quantitatively detect glutathione. This method constructs a high-performance colorimetric sensor by preparing a novel HOF-Fe nanozyme, which can realize rapid, high-sensitivity and high-specificity detection of glutathione, and can be widely used in the determination of glutathione content in actual samples.