32 results about "GeneQuant" patented technology

The invention provides a method for detecting Escherichia coli in milk based on an ultraviolet-visible spectrum technology. Under the complex background environment similar to that of the milk, the problems that detection spectra of microorganisms are affected by unfavorable factors, the phenomena of characteristic wavelength red shift, obvious noise and the like are generated, consequently spectral characteristics are difficult to pick up, and the accuracy of quantitative detection results is low can be effectively solved. The method comprises the steps that firstly, Escherichia coli powder is activated and subcultured, and a to-be-detected bacterium suspension is prepared; an ultraviolet spectrophotometer obtains ultraviolet-visible spectra of the to-be-detected bacterium suspension at different culture times; noise interference is eliminated through a standard normal correction method and an S-G convolution smoothing method; the spectral characteristic red shift phenomenon is explained from the angle of a formation mechanism of a benzene ring conjugated structure, and thus the characteristic waveband range is determined; the characteristic wavelengths are picked up in the characteristic waveband through a continuous projection algorithm, and the characteristic wavelengths are effectively screened according to the secondary growth law of the microorganisms; and the model relationship between the characteristic wavelengths and the total number of the microorganisms is established through a partial least squares method, and the total number of the Escherichia coli in the milk is quantitatively analyzed

The invention discloses a reovirus-detecting fluorescence quantitative PCR kit and an application thereof. The kit comprises: a) an RNA extraction solution, b) a reverse transcriptase reaction solution, c) reverse transcriptase, d) an RNA enzyme inhibitor, e) a primer and a TaqMan probe, f) a standard positive DNA template and g) a PCR fluorescence quantitative reaction solution. The kit is characterized in that: primer sequence is a sense primer: 5'-TGCGCCTATCCTTGAGTTGA-3', and an antisense primer: 5'-TTGCCAGGAAATACGGGTCT-3', and the size of an amplicon is 138 bp; the sequence of a fluorescence probe is: 5'-FAM-TCAAAATGGTGGACTTCAGTTTCGATTT-TAMRA-3', the 5' end of the probe is marked with a fluorescence emission group FAM, the 3' end is marked with a fluorescence quenching group TAMRA, the standard positive DNA template transforms a colon bacillus DH5 alpha by a pGEM-T carrier inserted with reovirus S1 protein zone 361 bp segment, plasmid is extracted after proliferation, an A260 ration is measured in an ultraviolet spectrophotometer, and the plasmid is diluted by 10 times of gradient. The kit can efficiently and conveniently monitor the reovirus pollution in serum products in real time, can be applicable to epidemiology investigation of reovirus infection, and can provide technical support for relevant fundamental researches, thus having quite broad application prospect.

The invention discloses a fluorescence quantitative PCR kit for detecting calf diarrhea virus and application. The kit comprises a) RNA extract, b) reverse transcriptase reaction liquid, c) reverse transcriptase, d) RNA enzyme inhibitor, e) primers and TaqMan probe, f) standard positive DNA template, and g) fluorescence quantitative PCR reaction liquid. The kit is characterized in that in a positive primer, a negative primer and a fluorescence probe sequence, a 5' end of the probe marks a fluorescence emitting group FAM, a 3' end of the probe marks a fluorescence quenching group TAMRA, the standard positive DNA template converts colon bacillus DH5a by a pGEM-T carrier inserted into a 185bp fragment of a calf diarrhea virus 5'-UTR area, plasmids are extracted after multiplication, and A260 is measured by an ultraviolet spectrophotometer to definite quantity and is diluted by 10 times of gradient. Preparation for the kit comprises the following steps: A, treatment of a specimen and a standard product; and B, RT-PCR amplification by a two-step method and real-time fluorescence detection, and application of the fluorescence quantitative PCR kit in medicaments for quantitatively detecting calf diarrhea virus. The quantitative result is more accurate, reliable and stable, and the repeatability is good.

The invention discloses a bovine parainfluenza type-3 virus detecting fluorescence quantitative PCR kit and an application thereof. The kit comprises: a) an RNA extraction solution, b) a reverse transcriptase reaction solution, c) reverse transcriptase, d) an RNA enzyme inhibitor, e) a primer and a TaqMan probe, f) a standard positive DNA template, and g) a fluorescence quantitative PCR reaction solution. The kit is characterized in that primer sequence is respectively a sense primer and an antisense primer: the size of an amplicon is 129 bp; in the sequence of a fluorescence probe, the 5' end of the probe is marked with a fluorescence emission group FAM while the 3' end is marked with a fluorescence quenching group TAMRA; the standard positive DNA template transforms a colon bacillus DH5 alpha by a pGEM-T carrier inserted with bovine parainfluenza type-3 virus conservative F protein coding zone 188 bp segment, plasmid is extracted after proliferation, an A260 ration is measured in an ultraviolet spectrophotometer, and the plasmid is diluted by 10 times of gradient. The result of the kit is more accurate, reliable, stable and repeatable. The kit can also be applied to the epidemiology investigation of bovine epidemic influenza and provide technical support for relevant fundamental researches, thus having quite broad application prospect.

The invention discloses a rabies virus detecting fluorescence quantitative PCR kit and an application thereof. The kit comprises: a) an RNA extraction solution, b) a reverse transcriptase reaction solution, c) reverse transcriptase, d) an RNA enzyme inhibitor, e) a primer and a TaqMan probe, f) a standard positive DNA template, and g) a PCR fluorescence quantitative reaction solution. The kit is characterized in that: primer sequence is a sense primer: 5'-TAGGATGCTATATGGGTCAAGTCAGA-3', and an antisense primer: 5'-TTCAAATGTCCCTTTCCCGAAGAA-3', and the size of an amplicon is 125 bp; the sequence of a fluorescence probe is: 5'-FAM-CAACGGTTATTGCTGCATGTGCTCCTGA-TAMRA-3', the 5' end of the probe is marked with a fluorescence emission group FAM, the 3' end is marked with a fluorescence quenching group TAMRA, the standard positive DNA template transforms a colon bacillus DH5 alpha by a pGEM-T carrier inserted with rabies virus N protein zone 391 bp segment, plasmid is extracted after proliferation, an A260 ration is measured in an ultraviolet spectrophotometer, and the plasmid is diluted by 10 times of gradient. The kit can efficiently and conveniently monitor the rabies virus pollution in serum products in real time, and can provide technical support for relevant fundamental researches, thus having quite broad application prospect.

The invention provides an indirect ELISA (enzyme-linked immunosorbent assay) detection kit based on toxoplasma gondii matrix protein 1. The indirect ELISA detection kit consists of a 96-hole elisa plate, a substrate coating substance, a standard substance, a reference substance, a substrate solution, skim milk powder, a sample diluent, washing liquid, reaction liquid, color developing liquid and stopping liquid. The indirect ELISA detection kit can identify IgGs of a variety of mammals such as humans, rabbits, pigs, dogs, cats, monkeys, mice, etc., can detect infection of toxoplasma gondii relatively rapidly, is high in specificity and sensitivity, and especially in large-scale clinical detection, can greatly improve the detection efficiency; in a detection method, an expensive PCR detector and other equipment are not required, but only an UV spectrophotometer, a thermostat and other equipment are needed; the indirect ELISA detection kit is simple in operation, high in specificity, good in repeatability and clear and stable in result; a sample to be detected is very easy to obtain.

The invention discloses a method for measuring millet seed vigor based on an ultraviolet spectrophotometer technology. The light absorption value of a seed lixivium is determined by adopting an ultraviolet spectrophotometer detection technology according to the principle of amino acid exosmose because the integrity of a cell membrane is damaged during a storage process of seeds; the storage years are more, and the exosmosis organic electrolytes are more, so that the light absorption value is higher. According to the method, the millet seed vigor can be detected quickly; the sample soaking time are found, and the sample determination wavelengths are screened to determine a seed vigor standard; the detection accuracy is high; the time consumption is short; the millet seeds with strong vigor have a low light absorption value; the millet seeds with weak vigor have a strong amino acid outflow effect and have a high light absorption value; the evaluation method is simple and reliable.

The invention provides a method for detecting zymoprotein residues in cefprozil prepared by an enzymatic method. The method is characterized by comprising the following steps: preparing a Coomassie brilliant blue G-250 dye solution and a standard protein stock solution; preparing a standard curve solution by taking a sodium bicarbonate solution as a solvent; preparing a blank solution by taking asodium bicarbonate solution as a solvent; preparing a test sample determination solution by taking a sodium bicarbonate solution as a solvent; drawing an absorbance-protein concentration standard curve through blank solution correction by using an ultraviolet spectrophotometer; and calculating the residual content of zymoprotein contained in the test sample according to the absorbance value read by the test sample determination solution. Sodium bicarbonate is ingeniously selected as a solvent, cefprozil can be dissolved, zymoprotein can be fully dissolved, the residual quantity of zymoproteinin a cefprozil product prepared through an enzymatic method can be effectively measured, the medication safety of medicine is improved, and the detection method is easy to operate, high in accuracy and good in reproducibility and reliability.

The invention discloses a culture medium for enrichment of lactate-utilizing bacteria. The culture medium contains a carbon source, a nitrogen source, promoters for the lactate-utilizing bacteria, aninorganic salt and vitamins; the promoters for the lactate-utilizing bacteria are lactic acid and antibiotics, and the vitamins are hemin, biotin, vitamin B12, para-aminobenzoic acid, folic acid and vitamin B6; and stable lactate-utilizing bacteria flora can be obtained by utilizing the culture medium, and the concentration of the lactate-utilizing bacteria is measured to be 1 OD by using an ultraviolet spectrophotometer at a wavelength lambda of 580 nm; and the lactate-utilizing bacteria can improve enteritis. The culture medium disclosed by the invention is developed and utilized for the first time; the lactate-utilizing bacteria are domesticated by using gradient lactic acid; and infectious microbes are inhibited by using the antibiotics.

The invention relates to a near-infrared fluorescent probe for detecting selenocysteine (Sec), and concretely relates to an organic compound based on cyanine and an application thereof. A structural formula of the organic compound based on the cyanine is shown as a formula I, the structural formula of the compound is shown as the formula I in the specification, and the compound is taken as a fluorescent probe of Sec. The Sec fluorescent probe compound can generate obvious displacement of corresponding fluorescence wavelength under existence of Sec, which can be used for Sec detection, interference by external detection condition is greatly reduced, and detection precision is increased. Under existence of Sec, according to the Sec fluorescent probe compound, the ultraviolet absorption generates obvious change, and an ultraviolet spectrophotometer and naked eyes are simultaneously used for detection. The compound as the fluorescence probe can be used for detecting the Sec level inside and outside cells, and has important biomedical meaning on a deep research a dynamics mechanism of generation, conveying and accumulation of Sec in organism, especially the research of physiological effect of Sec in mitochondria.

The invention discloses a method for detecting content of hypoxanthine in fish, and relates to a method for detecting hypoxanthine. The method disclosed by the invention is simple, fast in completion speed, reasonable in design, high in accuracy degree, good in data reference and easy to operate, the detection purpose can be achieved without special testing instruments, that is, by using conventional detection instruments, large-scale popularization and application can be facilitated. The method disclosed by the invention comprises the following steps: preparing a sample liquid from fish meat, performing coupling catalysis on the sample liquid by using horse radish peroxidase and xanthine oxidase, and detecting by using an ultraviolet spectrophotometer, so as to obtain the content of the hypoxanthine. The method is adopted to judge the freshness of fish.

The invention discloses a T3 DNA ligase-based cadmium ion detection method, which comprises the following steps: designing a DNA template, and when a target object cadmium ion exists, reducing the distance between two ends of a chain by the target object, so that a 3'end and a 5 'end are complementarily paired to form a blunt end; under the action of T3 DNA ligase, cyclizing the template; adding a section of oligonucleotide chain complementarily paired with the annular template as a primer for subsequent amplification reaction; under the action of adding phi 29 DNA polymerase, starting a rolling circle amplification reaction to obtain a longer nucleotide chain; adding a fluorescent dye SYBR Green I into the obtained product, inserting the dye into a DNA chain, detecting the sample by using an ultraviolet spectrophotometer under the excitation wavelength of 517-537 nm, and then measuring the fluorescence intensity under the excitation wave of 487-507 nm; detecting the cadmium ions by using a Real-Time PCR method, and the accuracy of the method disclosed by the invention is verified.

The invention provides an enzyme-assisted hairpin probe remodeled label-free colorimetric sensor based on target trigger and an application of the sensor. The invention also provides a method for visual detection of nucleic acid. According to the method, the nucleic acid is used as a target, a reaction is performed with the help of enzymes, G-quadruplex sequences that can produce colorimetric signals are formed through multiple ways, and the G-quadruplex sequences can be used for visual detection of the nucleic acid; the method controls the reaction conditions, a hairpin probe (HGP) opened bya target gene under the action of a T4 DNA ligase is connected with another double-stranded DNA (DGP) to complete remodeling, under the synergy of a DNA polymerase and a restriction enzyme, the targetDNA is recycled, and multiple channels for generating the G-quadruplex sequences are generated. According to the method, the enzymes are used to assist the DNA reaction to form a large amount of G-quadruplexes, and the G-quadruplexes can be used for detection by using an ultraviolet spectrophotometer, and can be used for visual detection by using naked eyes; and the detection method is convenient, the materials used in the method are commercialized, and the biological safety is high.

The invention discloses a pretreatment method for determination of 18 amino acids in C57 mouse brain tissue and plasma and belongs to the field of pretreatment of endogenous substances in biological samples. According to the method, an ultraviolet spectrophotometer and a high performance liquid chromatograph are used, a method for treating brain tissue homogenate and plasma is investigated by combining liquid-liquid extraction with a protein precipitation method, and the method mainly investigates the influence of a sample pretreatment method on the removal amount of proteins, the removal amount of total lipids and the extraction amount of 18 amino acids in a sample, and further optimizes to obtain the optimal pretreatment method for determining 18 amino acids in C57 rat brain tissues andplasma.

The invention discloses a heterometrus spinifer poison antibacterial polypeptide gene and a preparation method and application thereof. The preparation method comprises the following steps of: A, constructing a heterometrus spinifer poison gland cDNA library which comprises separation of total RNA by poison gland thereof, grinding caudal gland into fine powder in liquid nitrogen, and preparing spinifer poison gland total RNA through a Trizol method; B, separating and purifying mRNA by adopting a PolyATractmRNA separation system through the prepared spinifer poison gland total RNA, and measuring the prepared spinifer poison gland mRNA by using an ultraviolet spectrophotometer; C, using RNA as an initial amount, and synthesizing a first chain and a second chain of cDNA; D, continuously connecting double-chain cDNA with a SalI adapter, digesting the double-chain cDNA through NotI, and removing excessive SalI adapters and restriction enzyme cutting fragments from double-chain cDNA molecules; and E, connecting the double-chain cDNA with a pSPORT1 vector, identifying the poison gland cell cDNA library, selecting clones from the heterometrus spinifer poison gland cell cDNA library to prepare the spinifer poison antibacterial polypeptide gene. The method has the advantages of operability, low cost and high water solubility, and the heterometrus spinifer antibacterial polypeptide has the advantages of high sterilization speed and good effect.

The invention discloses a preparation method of parallel-configuration G-quadruplex DNA probe for specific detection, and belongs to the technical field of biological probes. The specific structural formula of the probe is shown in the specification. The probe is simple in preparation steps, easily available in raw materials and low in fluorescence background. Through the ultraviolet spectrophotometer and the fluorescence spectrophotometer, rapid bifunctional specific detection of the parallel configuration G-quadruplex DNA can be realized. The defects that a traditional detection method is single, and G-quadruplex DNA of different configurations is difficult to distinguish are overcome, and the method has wide application prospects in the fields of biochemistry, molecular biology and thelike.

The invention belongs to the technical field of biochemical analysis. The invention particularly relates to a multi-target simultaneous detection method based on concentration change of latex microspheres. Nanometer magnetic particles are used as magnetic separation carriers, and the surfaces of the nanometer magnetic particles are coupled with capture antibodies of different target objects; latex microspheres with different particle sizes are used as signal probes, the surfaces of the latex microspheres are coupled with detection antibodies or complete antigens of different target objects, and each particle size corresponds to one target object; the magnetic separation carriers, the signal probes and a to-be-detected target object are subjected to immune reaction; after magnetic separation, a signal probe solution which does not participate in reaction in a separation solution is collected; meanwhile, the maximum absorbance corresponding to the ultraviolet absorption peaks of the signal probes with different particle sizes is measured, and the corresponding target content is calculated according to the change of the absorbance. According to the method, multiple targets can be detected simultaneously, the detection time is greatly shortened, and an ultraviolet spectrophotometer is simple and convenient to operate, high in sensitivity, good in stability and low in cost.

The invention discloses a method for constructing a perilla frutescens core germplasm resource library based on an SRAP molecular marker, and belongs to the technical field of genetic diversity evaluation of perilla frutescens germplasm resources and construction of core germplasm. The method comprises the following steps of screening out 100 parts of perilla frutescens variety materials, planting the perilla frutescens variety materials in a flowerpot, taking seedling plant leaves as test materials, extracting total DNA of each variety, detecting the purity and integrity of the total DNA of each variety by using 1% agarose gel electrophoresis, determining the light absorption values of the total DNA of each variety at 260nm and 280nm by using an ultraviolet spectrophotometer, determining the purity and concentration of the DNA, and storing at -20 DEG C for later use; and carrying out SRAP-PCR amplification by using the total DNA, and carrying out electrophoretic separation on the amplification product by using 6% polyacrylamide gel. According to the method, a fingerprint spectrum is constructed for 100 parts of perilla frutescens germplasm resources by utilizing an SRAP marking technology, a perilla frutescens molecular retrieval table is compiled, core germplasm construction is carried out, the genetic relationship between perilla frutescens species and cultivated varieties is disclosed, a scientific classification system is established, and genetic diversity evaluation is carried out on the perilla frutescens perilla species and cultivated varieties.

The invention belongs to the technical field of food detection, and discloses a method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel. The method comprises the following stepsof constructing the nucleic acid hydrogel by taking T-2 toxin aptamer as a linker cross-linking agent, wherein the horseradish peroxidase is uniformly embedded in the nucleic acid hydrogel; adding a to-be-detected sample into the nucleic acid hydrogel, wherein the concentration of the T-2 toxin in the to-be-detected sample is 0.01ng mL<-1>-10000ng mL<-1>, and the T-2 toxin aptamer is combined withthe T-2 toxin, the gel is broken to release the horseradish peroxidase; and enabling the horseradish peroxidase to catalyze hydrogen peroxide and potassium iodide within time to react to generate iodine simple substance etched gold nanorods, and computing the content of the T-2 toxin according to an absorption peak of the gold nanorods under an ultraviolet spectrophotometer. The method disclosedby the invention is convenient and sensitive to detect.

The invention relates to a near-infrared fluorescent probe for detecting a superoxide anion free radical (O2<.>), in particular to a cyanine-based organic compound and application thereof. The compound is shown as a structural formula I and is taken as the O2<.> fluorescent probe. The compound used as the O2<.> fluorescent probe has the benefits that under the existence of O2<.>, a corresponding fluorescence wavelength obviously displaces, and the compound can be used for O2<.> detection and can greatly reduce the interference from external detection conditions, so that the detection precision is improved; under the existence of the O2<.>, the ultraviolet absorption is also changed obviously, and an ultraviolet spectrophotometer and naked eyes can be simultaneously used for detection; the compound can be used for detection on intracellular and extracellular O2<.> levels and has important biomedical significances in deeply studying a dynamic mechanism on production, conveyance and accumulation of the O2<.> in organisms and particularly in studying a physiological role of the O2<.> in mitochondria.

The invention discloses a method for effectively detecting the protein concentration in an available chlorine-containing solution, which comprises the following steps of: detecting the content of detected protein in a corneal contact lens cleaning solution by using a trace ultraviolet spectrophotometer, and setting an experimental group and a parallel control group, wherein the experimental group utilizes cleaning equipment to cooperate with an electrophoretic dissociation liquid to perform electrophoretic dissociation on a certain amount of detected protein samples, after the electrophoretic dissociation is completed, the content of protein in the electrophoretic dissociation liquid is detected through the trace ultraviolet spectrophotometer, and the parallel control group utilizes the cleaning equipment to perform electrophoretic dissociation on the electrophoretic dissociation liquid; and the parallel control group is used as a background control group of the experimental group. According to the method for effectively detecting the protein concentration in the available chlorine-containing solution, the elution content of lens tear protein can be quantitatively and qualitatively detected, the deproteinization capability of lens cleaning equipment is proved, experimental detection data is provided for a user, and the consumption and use safety of the user is guaranteed.

The invention discloses a method for detecting hot spot mutation of a human TERT gene promoter. The method comprises the following steps: a, enriching templates by adopting DNA enrichment conventionalPCR aiming at different specimens; b, purifying the PCR product, determining the concentration by using an ultraviolet spectrophotometer, and performing concentration calibration and standard substance preparation; and c, carrying out TERT-146 (C / T) and TERT-124 (C / T) mutation rate quantitative detection on the calibrated specimens by adopting qPCR. According to the method, a qPCR technology platform which is conventionally developed clinically is adopted, the high-sensitivity TERT hot spot mutation rate quantitative detection method is established for DNA specimens from various sources, thelower limit of the detected mutation rate reaches 0.05%, and the method has the advantages of being high in detection sensitivity, high in specificity, capable of accurately and quantitatively detecting the mutation rate, low in cost, suitable for conventional clinical development and the like.

The invention discloses a preparation method of a DNA probe for specifically detecting a parallel configuration G-quadruplex, belonging to the technical field of biological probes. The specific structural formula related to the probe is as follows: the preparation steps of the probe are simple, the raw materials are easy to obtain, and the fluorescence background is low. The rapid bifunctional specific detection of G-quadruplex DNA in parallel configuration can be realized by UV spectrophotometer and fluorescence spectrophotometer. It overcomes the shortcomings of traditional detection methods that are single and difficult to distinguish different configurations of G-quadruplex DNA, and has broad application prospects in biochemistry, molecular biology and other fields.

The invention provides a method for measuring purine with an ultraviolet spectrophotometer. The steps include: accurately weighing 0.5 g of fully dried (dried by freeze dryer) chyme sample, and placing it in a 25 ml test tube with stopper and scale; adding 2.5 ml of perchloric acid, and placing it in a constant temperature water bath at 90-95 °C Incubate for 1 hour; then add 17.5ml of acetic acid buffer, place it again in a constant temperature water bath at 90-95°C and incubate for 10-15 minutes, then filter; transfer 0.5ml of the filtrate to a centrifuge tube with a volume of 15ml, and centrifuge at 4000rpm for 15 minutes ; be placed in a constant temperature water bath of 90-95 ℃ and cultivated for 30 minutes; use a UV spectrophotometer to compare color at 260nm, and the measured purine concentration is represented by RNA equivalent; the invention is easy to operate, high in repeatability, and accurate in value Spectrophotometer was used to measure the content of purine in digesta and the content of microbial protein in duodenal digesta, which provided a methodological basis for determining the content of metabolic protein in dairy cows.

The invention relates to the technical field of polypeptide decoloration, in particular to a method for measuring the decoloration effect of a protein polypeptide solution. The method comprises the following steps: (1) dissolving dry polypeptide samples before and after decoloration in ultrapure water or deionized water according to the same concentration ratio to obtain a sample solution, standing at room temperature for a certain time, respectively scanning the sample solution and the ultrapure water at 380nm-780nm at an interval of 1-10nm by adopting a microplate reader or an ultraviolet spectrophotometer, and measuring the absorbance of the sample solution and the ultrapure water in the range; (2) making a wavelength-absorbance broken line graph; and (3) determining the decolorization effect according to the change trend of the broken line graph or the calculated decolorization rate through the change of qualitative or quantitative indexes. The method for measuring the decolorization effect of the protein polypeptide solution makes up the defects in the research in the prior art, the decolorization effect is observed more visually, and the method is suitable for solutions which are difficult to distinguish visually and have small differences.

The invention relates to the field of chemical measurement, in particular to a method and system for measuring the anti-Xa factor titer of a low-molecular-weight heparin calcium injection.The method comprises the steps that 100 microliters of a low-molecular-weight heparin calcium solution and 100 microliters of an antithrombin solution are taken and evenly mixed, balancing is conducted for 1 minute at the temperature of 37 DEG C, and a first mixed solution is obtained; adding 200 [mu] l of an FXa solution into the first mixed solution, uniformly mixing, and balancing at 37 DEG C for 1 minute to obtain a second mixed solution; adding 500 [mu] l of a chromophoric substrate solution into the second mixed solution, mixing, and balancing at 37 DEG C for 4 min to obtain a test solution; continuously measuring the absorbance of the test solution at the wavelength of 405nm by using an ultraviolet spectrophotometer and taking water as a blank, recording the current absorbance every one minute, totally 5 minutes, and calculating the absorbance change rate; 100 [mu] l of a trihydroxymethyl aminomethane-sodium chloride buffer solution and 100 [mu] l of a test solution are taken to replace a low-molecular-weight heparin calcium solution to be operated according to the steps, the absorbance change rates are calculated respectively, operation is more convenient, and the working efficiency is improved.

The invention discloses a fluorescent quantitative PCR kit for detecting bovine adenovirus type 3 and its application. The kit contains: a) a DNA extraction reagent, b) a hot-start Taq DNA polymerase, c) a primer and a TaqMan probe, d) ) Standard positive DNA template, e) PCR fluorescent quantitative reaction solution, characterized in that: the primer sequences are respectively sense primer: 5'-CCTGAATTCTCTTGCAGCCAGA-3', antisense primer: 5'-CCTACCGAACCGACGCAGAT-3', and the amplicon size is 100bp, the fluorescent probe sequence is: 5′-FAM-TGAGAAGGTACTCCTCGTCGCTGGACCA-TAMRA-3′, the 5′ end of the probe is labeled with the fluorescent emitting group FAM, and the 3′ end is labeled with the fluorescent quenching group TAMRA, and the standard positive DNA template is prepared by The pGEM-T vector inserted with the 100bp fragment of the adenovirus type 3 pol protein was transformed into Escherichia coli DH5α, and after multiplication, the plasmid was extracted and prepared, and quantified by measuring A260 with a UV spectrophotometer and 10-fold serial dilution. Efficient and convenient real-time monitoring of bovine adenovirus type 3 contamination in serum products can be widely used in epidemiological investigations of the virus infection, and can also provide technical support for related basic research, with broad application prospects.

The invention discloses a culture medium for enrichment of lactate-utilizing bacteria. The culture medium contains a carbon source, a nitrogen source, promoters for the lactate-utilizing bacteria, aninorganic salt and vitamins; the promoters for the lactate-utilizing bacteria are lactic acid and antibiotics, and the vitamins are hemin, biotin, vitamin B12, para-aminobenzoic acid, folic acid and vitamin B6; and stable lactate-utilizing bacteria flora can be obtained by utilizing the culture medium, and the concentration of the lactate-utilizing bacteria is measured to be 1 OD by using an ultraviolet spectrophotometer at a wavelength lambda of 580 nm; and the lactate-utilizing bacteria can improve enteritis. The culture medium disclosed by the invention is developed and utilized for the first time; the lactate-utilizing bacteria are domesticated by using gradient lactic acid; and infectious microbes are inhibited by using the antibiotics.

The present invention relates to the technical field of uric acid content in poultry feces, in particular to a method for measuring uric acid content in poultry feces by ultraviolet spectrophotometry, comprising the following steps: S1. Select a fecal raw material sample with a fixed quality, dry it in the sun, remove impurities, and pulverize it. Then use a sieve to filter; S2, add quantitative glycine buffer solution to the feces raw material to form a mixed sample for testing; S3, heat the sample in a water bath for 20 minutes to hydrolyze; S4, hydrolyze the sample , diluted to 15 times the volume with 7.35% PCA, then zero-adjusted with PCA: water=1:1; S5, detect the sample after zero-adjusted by an ultraviolet spectrophotometer, measure the absorbance value, the ultraviolet spectrophotometric method of the present invention The requirements for equipment and testing personnel are low, and the method has good stability and repeatability, so it can be used for qualitative measurement in front-line production.

The invention relates to a hydrogen sulfide colorimetric sensor for targeted induction of mimic enzyme inactivation based on a mixed node metal organic framework material, and belongs to the technical field of biosensor environment detection. The synthetic Cu-Fe MOFs with mixed nodes are used as an artificial mimic enzyme to catalyze H2O2 to oxidize 3,3,5,5-tetramethylbenzidine (TMB) to generate oxTMB, and the solution is changed from colorless to blue; when dissolved H2S is added, H2S and Cu 2+ have strong binding capacity under an acidic condition; therefore, the mimic enzyme is inactivated, so that the capability of oxidizing TMB by H2O2 is weakened. then, along with the increase of the H2S concentration, the catalytic activity of the mimic enzyme is gradually reduced, and the color is obviously and gradually lightened, so that the qualitative or quantitative detection of H2S can be realized through an ultraviolet spectrophotometer or the color change. Based on the concerted catalysis of Cu-Fe in the Cu-Fe MOFs, the sensitivity and selectivity of H2S determination are improved. The method has a good application prospect in water quality analysis and environmental protection.