This invention belongs to the field of
biochemical detection technology and discloses a method for detecting the content of the biomarker GSH based on TMB colorimetric method. The specific technical solution is as follows: Step 1:
Hydrogen-bonded organic framework is synthesized by hydrothermal method, and the
transition metal Fe is introduced for modification to obtain HOF-Fe material; Step 2:
Acetic acid-
sodium acetate solution is used as background solution, TMB solution and H2O2 solution are added respectively, and finally HOF-Fe is added to react. The
peroxidase-like activity of the material is measured by
ultraviolet spectrophotometer; Step 3:
Glutathione solution of different concentrations is added to the above
reaction system to quantitatively detect
glutathione. This method constructs a high-performance
colorimetric sensor by preparing a novel HOF-Fe nanozyme, which can realize rapid, high-sensitivity and high-specificity detection of
glutathione, and can be widely used in the determination of
glutathione content in actual samples.