Method for constructing perilla frutescens core germplasm resource library based on SRAP molecular marker

Abstract

The invention discloses a method for constructing a perilla frutescens core germplasm resource library based on an SRAP molecular marker, and belongs to the technical field of genetic diversity evaluation of perilla frutescens germplasm resources and construction of core germplasm. The method comprises the following steps of screening out 100 parts of perilla frutescens variety materials, planting the perilla frutescens variety materials in a flowerpot, taking seedling plant leaves as test materials, extracting total DNA of each variety, detecting the purity and integrity of the total DNA of each variety by using 1% agarose gel electrophoresis, determining the light absorption values of the total DNA of each variety at 260nm and 280nm by using an ultraviolet spectrophotometer, determining the purity and concentration of the DNA, and storing at -20 DEG C for later use; and carrying out SRAP-PCR amplification by using the total DNA, and carrying out electrophoretic separation on the amplification product by using 6% polyacrylamide gel. According to the method, a fingerprint spectrum is constructed for 100 parts of perilla frutescens germplasm resources by utilizing an SRAP marking technology, a perilla frutescens molecular retrieval table is compiled, core germplasm construction is carried out, the genetic relationship between perilla frutescens species and cultivated varieties is disclosed, a scientific classification system is established, and genetic diversity evaluation is carried out on the perilla frutescens perilla species and cultivated varieties.

Description

technical field [0001] The invention belongs to the technical field of genetic diversity evaluation and core germplasm construction of perilla germplasm resources, and specifically relates to a method for constructing a perilla core germplasm resource bank based on SRAP molecular markers. Background technique [0002] Basil (Perillafrutescens L.), also known as Huihuisu, Suma, Red Perilla, Chisu, Suzi, etc., belongs to the Lamiaceae Perilla genus annual herb, and is a traditional Chinese medicinal and edible plant. Its stems, Both leaves and seeds can be used as medicine, and the seedlings and young leaves have a unique fragrance. It is a favorite vegetable and condiment in East Asian countries. In recent years, studies have found that the content of α-linolenic acid (essential fatty acid) in perilla seed oil is as high as 65%, which has various health effects such as promoting brain development and treating cardiovascular and cerebrovascular diseases. extensive attention. ...

AI Technical Summary

Problems solved by technology

Due to the many types of plant variations in the genus Perilla, the large variation range, easy interspecific hybridization, and difficult identification of germplasm resources, the classification of many varieties is still controversial.

At present, most studies on the genetic diversity of Perilla genus focus on a small number of typical materials, focusing on the origin, kinship or classification, and seldom carry out comprehensive and systematic genetic diversity analysis. It is still not possible to fully understand the existing Genetic background and distribution rules and characteristics of genetic diversity of perilla germplasm resources

In terms of excavation and utilization of excellent germplasm, although some excellent germplasms have been obtained through phenotypic identification of some germplasms, in the face of such a large number of germplasm resources, there is a lack of an efficient and accurate screening of excellent germplasms with different characteristics. Strategies and methods of germplasm, thus increasing the difficulty of evaluation and utilization of germplasm resources

[0004] In addition, due to the influence of the market economy and the improper protection and utilization of perilla germplasm resources by the people, many excellent germplasms are gradually degrading, shrinking or even dying out.

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