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A kind of culture medium for enriching lactic acid-utilizing bacteria

A technology for enriching lactic acid and medium, applied in the direction of microorganism-based methods, methods using microorganisms, bacteria, etc., can solve the problems of enrichment and cultivation of lactic acid-utilizing bacteria, low content of lactic acid-utilizing bacteria, and failure to improve enteritis

Inactive Publication Date: 2020-12-11
山西医科大学汾阳学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of lactic acid-utilizing bacteria in the human intestine is low (<1.7%), which cannot achieve the purpose of improving enteritis, and there is no report on the enrichment and cultivation of lactic acid-utilizing bacteria

Method used

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  • A kind of culture medium for enriching lactic acid-utilizing bacteria
  • A kind of culture medium for enriching lactic acid-utilizing bacteria
  • A kind of culture medium for enriching lactic acid-utilizing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A culture medium for enriching lactic acid-utilizing bacteria, the 1 (g / 100ml) culture medium is prepared from the following raw materials in parts by weight:

[0071] Lactic acid 1g

[0072] Tryptone 1g

[0073] Yeast Dip Powder 0.25g

[0074] Sodium bicarbonate 0.4g

[0075] Cysteine ​​1g

[0076] Dipotassium hydrogen phosphate 0.045g

[0077] Potassium dihydrogen phosphate 0.045g

[0078] Sodium chloride 0.09g

[0079] Magnesium Sulfate Heptahydrate 0.09g

[0080] Calcium chloride 0.09g

[0081] Hemin 1μg

[0082] Biotin 1μg

[0083] Vitamin B 12 1μg

[0084] p-aminobenzoic acid 1μg

[0085] Folic acid 1μg

[0086] Vitamin B 6 1μg

[0087] Streptomycin 5.1 mg

[0088] Kanamycin 1.9mg.

[0089] Utilize the method for above-mentioned culture medium to cultivate lactic acid bacteria colony, be to realize by following steps:

[0090] (1) Take fresh fecal samples from healthy people, and inject CO 2 Under airflow conditions, dilute with phosphate buff...

Embodiment 2

[0127] A culture medium for enriching lactic acid-utilizing bacteria, the 1 (g / 100ml) culture medium is prepared from the following raw materials in parts by weight:

[0128] Lactic acid 1g

[0129] Tryptone 1.2g

[0130] Yeast Dip Powder 0.2g

[0131] Sodium bicarbonate 0.5g

[0132] Cysteine ​​0.8g

[0133] Dipotassium hydrogen phosphate 0.05g

[0134] Potassium dihydrogen phosphate 0.04g

[0135] Sodium chloride 0.10g

[0136] Magnesium Sulfate Heptahydrate 0.08g

[0137] Calcium chloride 0.10g

[0138] Hemin 1μg

[0139] Biotin 1μg

[0140] Vitamin B 121μg

[0141] p-aminobenzoic acid 1μg

[0142] Folic acid 1μg

[0143] Vitamin B 6 1μg

[0144] Streptomycin 4.9 mg

[0145] Kanamycin 2.1mg.

[0146] Utilize the method for above-mentioned culture medium to cultivate lactic acid bacteria colony, be to realize by following steps:

[0147] (1) Take fresh fecal samples from healthy people, and inject CO 2 Under airflow conditions, dilute with phosphate buffe...

Embodiment 3

[0159] A culture medium for enriching lactic acid-utilizing bacteria, the 1 (g / 100ml) culture medium is prepared from the following raw materials in parts by weight:

[0160] Lactic acid 1 g

[0161] Tryptone 0.8g

[0162] Yeast Dip Powder 0.3g

[0163] Sodium bicarbonate 0.3g

[0164] Cysteine ​​1.2g

[0165] Dipotassium hydrogen phosphate 0.04g

[0166] Potassium dihydrogen phosphate 0.05g

[0167] Sodium chloride 0.08g

[0168] Magnesium sulfate heptahydrate 0.10g

[0169] Calcium chloride 0.08g

[0170] Hemin 1μg

[0171] Biotin 1μg

[0172] Vitamin B 12 1μg

[0173] p-aminobenzoic acid 1μg

[0174] Folic acid 1μg

[0175] Vitamin B 6 1μg

[0176] Streptomycin 5 mg

[0177] Kanamycin 2mg.

[0178] Utilize the method for cultivating lactic acid bacteria colony of above-mentioned culture medium, be to realize by following steps:

[0179] (1) Take fresh fecal samples from healthy people, and inject CO 2 Under airflow conditions, dilute with phosphate buff...

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Abstract

The invention discloses a culture medium for enrichment of lactate-utilizing bacteria. The culture medium contains a carbon source, a nitrogen source, promoters for the lactate-utilizing bacteria, aninorganic salt and vitamins; the promoters for the lactate-utilizing bacteria are lactic acid and antibiotics, and the vitamins are hemin, biotin, vitamin B12, para-aminobenzoic acid, folic acid and vitamin B6; and stable lactate-utilizing bacteria flora can be obtained by utilizing the culture medium, and the concentration of the lactate-utilizing bacteria is measured to be 1 OD by using an ultraviolet spectrophotometer at a wavelength lambda of 580 nm; and the lactate-utilizing bacteria can improve enteritis. The culture medium disclosed by the invention is developed and utilized for the first time; the lactate-utilizing bacteria are domesticated by using gradient lactic acid; and infectious microbes are inhibited by using the antibiotics.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and relates to an improved technology for cultivating lactic acid-utilizing bacteria, in particular to a culture medium capable of enriching lactic-acid-utilizing bacteria. Background technique [0002] With the acceleration of people's life rhythm and the advancement of social aging, more and more people's stomach is in a sub-healthy state, and inflammatory bowel disease (IBD) often occurs. Inflammatory bowel disease is a complex group of nonspecific intestinal inflammatory diseases, including ulcerative colitis (UC) and Crohn's disease (CD). It is generally believed that the dysbiosis and dysfunction of intestinal flora is one of the most important factors in inducing ulcerative colitis, so the stabilization and improvement of intestinal flora is an urgent problem to be solved. [0003] Lactic acid bacteria are a large number of intestinal bacteria, including Lactobacillus, B...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/36A61K35/744A61P1/00C12R1/01
Inventor 刘威李榕王杰李欣欣王子光
Owner 山西医科大学汾阳学院
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