Method and system for determining anti-Xa factor titer of low-molecular-weight heparin calcium injection

A low-molecular-weight heparin and titer determination technology, which is applied in the field of low-molecular-weight heparin calcium injection anti-Xa factor titer determination methods and systems thereof, and can solve the problems of large experimental errors, cumbersome operations, and it is difficult to ensure that the reaction time of small tubes is consistent.

Pending Publication Date: 2022-04-05
江苏大同盟制药有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0005] The inventor found that there are following deficiencies in the actual inspection process: the reaction is carried out in 16 test tubes, the operation is cumber

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  • Method and system for determining anti-Xa factor titer of low-molecular-weight heparin calcium injection
  • Method and system for determining anti-Xa factor titer of low-molecular-weight heparin calcium injection
  • Method and system for determining anti-Xa factor titer of low-molecular-weight heparin calcium injection

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Embodiment Construction

[0028] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0029] In describing the present invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientation or positional relationship indicated by "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than Nothing indicating or implying that a referenced device or element...

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Abstract

The invention relates to the field of chemical measurement, in particular to a method and system for measuring the anti-Xa factor titer of a low-molecular-weight heparin calcium injection.The method comprises the steps that 100 microliters of a low-molecular-weight heparin calcium solution and 100 microliters of an antithrombin solution are taken and evenly mixed, balancing is conducted for 1 minute at the temperature of 37 DEG C, and a first mixed solution is obtained; adding 200 [mu] l of an FXa solution into the first mixed solution, uniformly mixing, and balancing at 37 DEG C for 1 minute to obtain a second mixed solution; adding 500 [mu] l of a chromophoric substrate solution into the second mixed solution, mixing, and balancing at 37 DEG C for 4 min to obtain a test solution; continuously measuring the absorbance of the test solution at the wavelength of 405nm by using an ultraviolet spectrophotometer and taking water as a blank, recording the current absorbance every one minute, totally 5 minutes, and calculating the absorbance change rate; 100 [mu] l of a trihydroxymethyl aminomethane-sodium chloride buffer solution and 100 [mu] l of a test solution are taken to replace a low-molecular-weight heparin calcium solution to be operated according to the steps, the absorbance change rates are calculated respectively, operation is more convenient, and the working efficiency is improved.

Description

technical field [0001] The invention relates to the field of chemical measurement, in particular to a method for measuring the anti-Xa factor potency of low molecular weight heparin calcium injection and a system thereof. Background technique [0002] Low molecular weight heparin calcium is a new type of antithrombin Ⅲ (ATⅢ) dependent antithrombotic drug, which is mainly used for the prevention and treatment of deep vein thrombosis, and can also be used to prevent blood clot formation during hemodialysis. [0003] Low-molecular-weight heparin calcium injection is a sterilized aqueous solution of low-molecular-weight heparin calcium. It is clinically used to prevent blood clot formation and deep vein thrombosis during hemodialysis. It has definite curative effect and is widely used. Although low-molecular-weight heparin calcium injection has good clinical efficacy, the pharmacokinetics of this product is determined by its plasma anti-Xa factor activity, and the measurement de...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/38
Inventor 葛晓进李继杰聂利芳
Owner 江苏大同盟制药有限公司
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