Method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel

A hydrogel and T-2 technology, applied in the field of rapid detection of T-2 toxin in food based on nucleic acid hydrogel, can solve the problems of long detection time, expensive equipment, high detection cost, etc., and achieve simple and good choice Effects on Sex and Sensitivity

Inactive Publication Date: 2020-01-31
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require expensive instruments and equipment, high detection costs, strict pretreatment of samples, and long detection time.

Method used

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  • Method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel
  • Method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel
  • Method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel

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Embodiment Construction

[0041] The present application will be further introduced below in conjunction with the embodiments.

[0042] In order to more clearly describe the embodiments of the present invention or the technical solutions in the prior art, in the following description, different "an embodiment" or "an embodiment" do not necessarily refer to the same embodiment. Different embodiments can be replaced or combined, and those skilled in the art can obtain other implementations based on these embodiments without any creative effort.

[0043] combine figure 1 , a kind of method based on nucleic acid hydrogel rapid detection T-2 toxin in food, comprises the steps:

[0044]Constructing a nucleic acid hydrogel using the T-2 toxin aptamer as a linker cross-linking agent, wherein horseradish peroxidase is evenly embedded in the nucleic acid hydrogel;

[0045] Add the test sample to the nucleic acid hydrogel, and the T-2 toxin concentration in the test sample is 0.01ng mL -1 -10000ng mL -1 , the...

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Abstract

The invention belongs to the technical field of food detection, and discloses a method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel. The method comprises the following stepsof constructing the nucleic acid hydrogel by taking T-2 toxin aptamer as a linker cross-linking agent, wherein the horseradish peroxidase is uniformly embedded in the nucleic acid hydrogel; adding a to-be-detected sample into the nucleic acid hydrogel, wherein the concentration of the T-2 toxin in the to-be-detected sample is 0.01ng mL<-1>-10000ng mL<-1>, and the T-2 toxin aptamer is combined withthe T-2 toxin, the gel is broken to release the horseradish peroxidase; and enabling the horseradish peroxidase to catalyze hydrogen peroxide and potassium iodide within time to react to generate iodine simple substance etched gold nanorods, and computing the content of the T-2 toxin according to an absorption peak of the gold nanorods under an ultraviolet spectrophotometer. The method disclosedby the invention is convenient and sensitive to detect.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for rapidly detecting T-2 toxin in food based on nucleic acid hydrogel. Background technique [0002] The description of the background technology of the present invention belongs to the relevant technology related to the present invention, and is only used to illustrate and facilitate the understanding of the content of the present invention. prior art on the filing date. [0003] In recent years, food safety problems related to mycotoxin residues have emerged in an endless stream. The common ones are trichothecenes, aflatoxins, zearalenone, etc., among which T-2 toxin is the most important type A toxin of trichothecenes. , is also the most toxic one. T-2 toxin mainly acts on tissues and organs with vigorous cell division, can inhibit the synthesis of DNA, RNA and protein, has strong genotoxicity, immunotoxicity, blood toxicity, carcinogenic teratogenicity, and...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/5308
Inventor 梁俊李双孙云凤陈瑞鹏
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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