Method for measuring decolorization effect of protein polypeptide solution

A protein peptide and solution technology, applied in the field of peptide decolorization, can solve problems such as indistinguishability, and achieve the effect of outstanding practicability and accuracy

Inactive Publication Date: 2022-02-08
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] The invention provides a method based on measuring the decolorization effect of protein polypeptide solution, which makes up for the disadvantages existing in the research of the prior art, and the o...

Method used

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  • Method for measuring decolorization effect of protein polypeptide solution
  • Method for measuring decolorization effect of protein polypeptide solution
  • Method for measuring decolorization effect of protein polypeptide solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] After the fresh oysters were cleaned and shelled, put them into a beater, add 2 times the quality of deionized water, break and mix evenly, take out the oyster liquid and add animal protease (A), whey protease (R), trypsin ( Y), flavor protease (F), compound protease (M) and alkaline protease (J), were enzymatically hydrolyzed for 1, 3, 5, 7, and 9 hours under corresponding pH and temperature conditions, and collected different enzymatic hydrolysis conditions The enzymatic hydrolyzate under the solution was inactivated in a boiling water bath for 10 minutes, and then freeze-dried.

[0070] The dried powder was redissolved in deionized water at 10% and 20%, and after standing at room temperature for 30 minutes, an appropriate amount of sample solution was scanned on a microplate reader at 380nm-780nm intervals of 10nm, and its absorbance in this range was measured.

[0071] The enzymatic hydrolysis conditions are shown in Table 1 below.

[0072] Table 1 Enzymolysis cond...

Embodiment 2

[0078] Due to the different colors of the polypeptide solution samples, this experiment uses watercolor pigments for the determination of the basic color of the solution, and at the same time verifies the feasibility of the established method and the explanation of related principles. Since the gouache pigment is easy to precipitate and the color is darker after dissolving, it affects the determination. The watercolor raw material has a certain degree of transparency, and the materials are gum arabic (adhesive), glycerin / sugar water (wetting agent), phenol (preservative), etc. These materials have an influence on the absorbance of the solution at 380nm-780nm. Smaller, so this experiment selects watercolor pigments for the determination of the experiment.

[0079] Dilute 36 kinds of watercolor pigments of different colors in deionized water according to 500, 1000, 2000, 4000, 8000 and 16000 times respectively. After standing at room temperature for 30 minutes, take an appropria...

Embodiment 3

[0085] Sturgeon skin was used as the raw material of the experiment in this example, 4 times the mass of deionized water was added, and compound protease was added to enzymolyze at 50°C for the same time, the enzyme was inactivated, and centrifuged to obtain the supernatant as sturgeon skin protein polypeptide solution.

[0086] Five kinds of activated carbon commonly used in industry were selected to decolorize the sturgeon fish skin protein polypeptide solution, adding activated carbon at 0.15%, 0.30%, 0.45% and 0.60%, respectively, and decolorizing at 50°C for 30 min, 60 min, 90 min and 120 min respectively , and use filter paper to filter, and add the same quality of food-grade diatomaceous earth as a filter aid. Put the sturgeon fish skin protein peptide solution obtained after suction filtration at room temperature for 30 minutes, then use a microplate reader or an ultraviolet spectrophotometer to scan the sample solution and ultrapure water at intervals of 10nm from 380...

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Abstract

The invention relates to the technical field of polypeptide decoloration, in particular to a method for measuring the decoloration effect of a protein polypeptide solution. The method comprises the following steps: (1) dissolving dry polypeptide samples before and after decoloration in ultrapure water or deionized water according to the same concentration ratio to obtain a sample solution, standing at room temperature for a certain time, respectively scanning the sample solution and the ultrapure water at 380nm-780nm at an interval of 1-10nm by adopting a microplate reader or an ultraviolet spectrophotometer, and measuring the absorbance of the sample solution and the ultrapure water in the range; (2) making a wavelength-absorbance broken line graph; and (3) determining the decolorization effect according to the change trend of the broken line graph or the calculated decolorization rate through the change of qualitative or quantitative indexes. The method for measuring the decolorization effect of the protein polypeptide solution makes up the defects in the research in the prior art, the decolorization effect is observed more visually, and the method is suitable for solutions which are difficult to distinguish visually and have small differences.

Description

technical field [0001] The invention relates to the technical field of polypeptide decolorization, in particular to a method based on measuring the decolorization effect of a protein polypeptide solution. Background technique [0002] A large number of studies have shown that peptides have physiological activities such as anti-tumor, anti-inflammatory, anti-bacterial, and anti-oxidant, and their functional properties are closely related to human health, and are widely used in the fields of medicine, cosmetics, and food. There are many relevant standards for polypeptides in China, but the requirements for its color are relatively low. For example, GB31645-2018 requires 1% collagen peptide solution to be white or light yellow; GB / T22729-2008, SB / T10634-2011, QB / T2879-2007 and QB / T4588-2013 require the color of peptide powder to be white or light yellow ; GB / T22492-2008 requires the color of soybean peptide powder to be white, light yellow, yellow; QB / T4707-2014 requires corn ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/314G01N2021/3148
Inventor 赵元晖陈泽凡韩贵新刘康徐新星许贺白帆刘宇辰郑耀文
Owner OCEAN UNIV OF CHINA
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