Method for the determination of uric acid in poultry manure by UV spectrophotometry

A spectrophotometry and spectrophotometer technology, applied in the measurement of color/spectral characteristics, etc., can solve the problems of chromatographic column damage, unsatisfactory chromatographic peaks, and complicated extraction methods.

Active Publication Date: 2021-10-01
HUAIYIN TEACHERS COLLEGE
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Problems solved by technology

[0005] The purpose of the present invention is to solve the traditional high performance liquid chromatography detection uric acid sample that exists in the prior art, the extraction method is loaded down with trivial details, the chromatographic peak is not ideal enough, mobile phase mostly uses phosphate, the shortcoming that damages the chromatographic column relatively, and proposes A method for the determination of uric acid in poultry feces by ultraviolet spectrophotometry

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  • Method for the determination of uric acid in poultry manure by UV spectrophotometry
  • Method for the determination of uric acid in poultry manure by UV spectrophotometry
  • Method for the determination of uric acid in poultry manure by UV spectrophotometry

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be apparent from the embodiments of the present invention, and embodiments, are intended, as described in the embodiments of the invention, not all of the embodiments of the invention.

[0019] In the present embodiment, the ultraviolet spectrophotometry is measured by the method of uric acid content in the bird feces, including the following steps:

[0020] S1, select 250 mg of fecal feedstock samples, dry dry, induce, pulverized, and then filter the screen by 40 purposes;

[0021] S2, adding a glycine buffer having a 100 mLPH value of 8.3 in a fecal feedstock to form a mixed sample for test;

[0022] S3, heat 20 min under the condition of 50 ° C water bath, and hydrolyzes;

[0023] S4, the hydrolyzed sample is diluted to 15 times to 15 times in 7.35% PCA, and then zero it with PCA: water = 1: 1;

[0024] S5, set the ultraviolet spectrophotometer to detect the zero-zero sample at a wavelength of 285 n...

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Abstract

The present invention relates to the technical field of uric acid content in poultry feces, in particular to a method for measuring uric acid content in poultry feces by ultraviolet spectrophotometry, comprising the following steps: S1. Select a fecal raw material sample with a fixed quality, dry it in the sun, remove impurities, and pulverize it. Then use a sieve to filter; S2, add quantitative glycine buffer solution to the feces raw material to form a mixed sample for testing; S3, heat the sample in a water bath for 20 minutes to hydrolyze; S4, hydrolyze the sample , diluted to 15 times the volume with 7.35% PCA, then zero-adjusted with PCA: water=1:1; S5, detect the sample after zero-adjusted by an ultraviolet spectrophotometer, measure the absorbance value, the ultraviolet spectrophotometric method of the present invention The requirements for equipment and testing personnel are low, and the method has good stability and repeatability, so it can be used for qualitative measurement in front-line production.

Description

Technical field [0001] The present invention relates to the field of uric acid content in the poultry feces, and more particularly to a method of determining the uric acid content in foeoferia in an ultraviolet spectrophotometry. Background technique [0002] With the continuous improvement of the people's living standards, people's demand for meat products, milk, and eggs have also present the trend year by year, which makes the animal husbandry have developed rapidly, but at the same time, a large number of livestock and poultry is also produced. It creates serious pollution. How to deal with livestock feces become a big problem facing major farms. [0003] Studies have shown that poultry feces protein content is 24% -30%, and there is a data show that due to the short intestines of avian animals, foods in their intestines are short, and more than 60.0% of poultry feed nutrients are directly discharged from the intestines. The crude protein is 21.8% -28.0%, which has a total am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 谢鹏蔡羽娟陶德军徐永刚刁恩杰汪沐
Owner HUAIYIN TEACHERS COLLEGE
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