174 results about "Ultraviolet spectrophotometry" patented technology

The present invention relates to method of purifying and detecting metal ion in dimetyl sulfoxide as the solution for polymerizing polyactylonitrile carbon fiber. The metal impurities are eliminated from dimetyl sulfoxide based on static adsorption and exchange principle, and the elimination includes eliminating iron ion from dimetyl sulfoxide with macroporous weak alkali type anion exchange resin and eliminating calcium, magnesium, sodium and potassium ions from dimetyl sulfoxide with macroporous strong base type cation exchange resin. The detection includes ultraviolet spectrophotometry to detect the concentrations of various metal ions in the organic solvent, o-phenanthroline colorimetry to detect iron ion content, chloroposphonazo I colorimetry to detect calcium and magnesium ion content, and 18-crown-6 synergistic extraction process to detect potassium ion content.

Belonging to the field of food analysis, the invention relates to a method for detection of tea polysaccharide in Pu'er tea or a Pu'er tea extract. The detection method comprises the steps of: 1. preparing a glucose standard solution; 2. preparing a Pu'er tea or Pu'er tea extract sample into a detection solution; 3. measuring the absorbance of the standard reference solution and the detection solution by ultraviolet spectrophotometry, and drawing a standard curve; and 4. calculating the content of tea polysaccharide in the Pu'er tea or the Pu'er tea extract.

The invention provides a quality detection method for tibetan medicine rhododendron anthopogonoide and a tibetan medicine rhododendron anthopogonoide preparation. The method includes: determining the total flavonoid content in the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation by ultraviolet-spectrophotometry; simultaneously determining the total quercetin content in the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation in the same chromatograph by high-performance liquid chromatography; and establishing a finger-print chromatogram of flavonoids in the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation by the high-performance liquid chromatography. Therefore, quality of the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation can be guaranteed by detecting whether common peaks exist in the finger-print chromatogram or not, and further medication safety for patients is guaranteed. The quality detection method is stable in sample solution, high in precision and excellent in reproducibility, has certain specificity, is good in separating effect of each characteristic constituent in the finger-print chromatogram, can be used for quality detection of rhododendron anthopogonoide medical materials, and is beneficial to guarantee of safety, effectiveness, controllability in quality and good clinical effect for the tibetan medicine rhododendron anthopogonoide and the tibetan medicine rhododendron anthopogonoide preparation.

The invention discloses a cell culture method used for accelerating biological synthesis of ganoderam lucidum karst secondary metabolite, which comprises the following procedures: inoculating ganoderam lucidum karst bacterial to the oblique plane culture medium in order to culture; proceeding with one-stage liquid breed culture, two-stage liquid breed culture and liquid submerged cultuse sequentially, wherein definite quantity induction zygotic which is prepared by funginite mycelium is added in order to induce the biological synthesis of ganoderam lucidum karst polysaccharide and ganoderam lucidum karst acid during the liquid submerged cultuse process. The ganoderam lucidum karst polysaccharide content and ganoderam lucidum karst acid content of cell culture are determined separately with concentrated sulfuric acid-phenol method and ultraviolet spectrophotometry method, the result shows that the method can accelerate he biological synthesis of ganoderam lucidum karst polysaccharide and ganoderam lucidum karst acid in the culture and provides the basis for industry production and commercial production of ganoderam lucidum karst polysaccharide and ganoderam lucidum karst acid.

The invention discloses a method for extracting lutein ester compounds form marigolds, relating to an extraction method for extracting natural and edible uranidin lutein ester compounds from dried flower particles of the marigolds by adopting column chromatography and comprising two steps of ultrasonic extraction and the column chromatography. The extraction rate of the obtained lutein ester compounds can achieve 8.4%-8.6%, the lutein ester compounds have favorable purity after being determined by ultraviolet spectrophotometry, and the color scale E474nm1% is approximate to 698.

The invention provides an ultraviolet spectrophotometry of the content of petroleum in soil. The method comprises the following steps: 1. preparing standard petroleum; 2. preparing purified petroleum ether; 3. weighting 15-25g of fresh soil in a beaker, adding 0.5-0.7ml of hydrochloric acid (1:1) for acidification, mixing, then adding 20-30g of a dry sample of MgSO4.H2O, then performing Soxhlet extraction with chloroform, after reflux, reclaiming chloroform, wherein residue is used as a standby for ultraviolet measurement; 4. weighting 5-10ml of petroleum ether to dissolve the residue, using petroleum ether as reference to place in a quartz cuvette, using a ultraviolet spectrometer to scan within 201-270mm, quantitatively comparing the absorbance of petroleum ether in 256nm of wavelength with that of the standard series of residual petroleum to calculate the content of petroleum; 5. drawing a standard curve; and 6. calculating the petroleum content of soil. The method of the invention avoids the influence of solid-phase differentiation on the measurement result of petroleum so that all the petroleum test data have comparability and the test accuracy is increased.

The invention discloses a detection method and system for total nitrogen and total phosphorus. The detection method comprises the following steps: heating a water sample to which potassium persulfate is added in an alkaline and closed environment, and performing digestion for 1-10 minutes after heating to 70-110 DEG C; and then changing the pH value of the water sample for realizing an acid environment, and continuing to perform digestion on the water sample at 125-250 DEG C for 1-10 minutes, thereby obtaining the water sample in complete digestion, wherein the corrosion to a testing vessel by a high-temperature alkaline environment is prevented, and the high temperature of 125-250 DEG C accelerates the digestion process of the water sample; then determining the total nitrogen content by utilizing an existing ultraviolet spectrophotometry; and adding an ammonium molybdate reagent to the water sample after complete digestion, and determining the content of the total phosphorus in the water sample by utilizing the ammonium molybdate spectrophotometry. Therefore, the total nitrogen and the total phosphorus can be detected sequentially after digestion of the sample without secondary water sample digestion; the detection time is shortened further; the detection efficiency is improved.

The invention relates to a detection method, in particular to a method for detecting a purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry. The method comprises the following steps of: a, preparing purple sweet potato anthocyanidin pigment extract; b, adjusting the purple sweet potato anthocyanidin pigment extract to be acidic; and c, under the maximum absorption wavelength of the purple sweet potato anthocyanidin pigment, detecting the absorbance of anthocyanidin pigment solution to be detected. The detection method is easy to operate, convenient to compute, and accurate in result, has low cost of used instruments and low detection cost, and is suitable for the frontline of production; meanwhile, the detection method is special for the purple sweet potato anthocyanidin pigment, and the invention provides an economic, visual and rapid qualitative and quantitative test for purple sweet potato anthocyanidin pigment production enterprises.

The invention belongs to a preparation technology of natural antioxidant, particularly relates to a preparation method of antioxidant in blueberry leaves. The preparation method of the antioxidant in the blueberry leaves includes the steps as follows: (1) collecting fresh blueberry leaves, washing the fresh blueberry leaves with water, draining away water, shearing the leaves into segments with different length, adding 8-14 times water, adjusting pH to 3-8, conducting extraction in hot-water bath at the temperature of 90-100 DEG C, filtering the extract, and decompressing and concentrating the extract to 15-25 times of original concentration at the temperature of 50-60 DEG C; and (2) conducting adsorption with macroporous resin, eluting with water and 10%-30% alcohol, detecting by ultraviolet spectrophotometry, collecting adsorbed eluent with strong antioxidant activity at 280nm part, decompressing and concentrating the collected eluent to paste, and conducting vacuum-drying at the temperature 50-60 DEG C, thus obtaining light brown powder. The preparation method is simple in technology, friendly to environment and good in purity; and the product is safe and has strong antioxidantactivity.

The invention discloses a method for measuring the content of residue acrylamide monomer in water treatment agent cationic polyacrylamide, and the method is realized through the following steps: firstly, immersing polymer ationic polyacrylamide in a mixed solvent (volume ratio 9 to 1) formed by ethanol and water for 24 hours, transferring supernatant fluid into a volumetric flask, replenishing a certain amount of ethanol and water to enable the volume ratio of ethanol to water to be 1 to 1, and performing the isochoric process by mixed liquid. The absorbance A of to-be-measured solution is measured at a position with 205nm wavelength through the ultraviolet spectrophotometry, and the content of acrylamide in the to-be-measured solution can be calculated according to a preliminary drawn acrylamide standard sample standard curve. According to the invention, the recovery rate of measured material achieves more than 85%, and the method has the advantages of simple operation, excellent reproducibility, cheap and non-toxic solvent, low measurement cost and the like, thereby being applicable to quality inspection by manufacturing enterprises, use and application divisions and quality inspection divisions.

The invention discloses a dissolution method of tablets containing (+)-1-(3-Ethoxy-4-methoxyphenyl)-2-(methylsulfonyl) ethylamine N-acetyl-L-leucine salt. According to the technical scheme, a lauryl sodium sulfate aqueous solution with the concentration being 0.1%-1.0% is used as dissolution media, the dissolution temperature is 37 DEG C, a paddle method is used by 50-75 r/min, ultraviolet spectrophotometry is adopted, and the absorption degree is determined at 230 nm. Through the method, the dissolution behavior and the preparation mass of a reaction preparation can be reflected objectively. Meanwhile, the invention discloses a preparation technology of the tablets. The preparation technology includes the steps of sieving, mixing, dry granulating, total blending, tabletting, coating and packaging for being put in storage. The sticking problem of the tablets and the problem of being poor in content uniformity in the production process can be well solved.

The invention is a method for measuring the content of triterpenes compound in Ganoderma Lucidum, especially a method for using ultraviolet splitting luminosity to measure, the invention refers to biology technology field. The technology project is: the Ganodeerma Lucidum is put into a cleaned tube, adds chloroform to immerse for 0.5-1 hours, and acquires the supernate after being centrifugated, uses the saturate NaHCO3 solvent to extract for 2-3 times, collects the NaHCO3 layer, acquires the liquid for test, measures the volume accurately, uses ultraviolet splitting luminosity method to measure the light absorbing valve under 250-260nm wavelength, works out the content of triterpenes in the sample according to the standard curve. The method saves the acidification, chloroform extraction, reduced pressure distillation and drying steps, the period of measurement is shortened greatly.

The invention discloses a method for detecting cyanide ions based on gold nanorods, comprising the following steps: preparing gold nanorod solution, reacting the gold nanorod solution with a series of cyanide ion standard solutions, drawing a working curve through linear variations of ultraviolet absorption spectrum and/or two-photon excited fluorescent spectrum to obtain a linear equation with one unknown, measuring a sample by a corresponding scanning method, and taking a spectral value of the sample to the linear equation with one unknown for the standard working curve to calculate a sample concentration value. The preparation process of the gold nanorods is simple and convenient and is nontoxic and environment-friendly, the synthesized gold nanorods are uniform in size and good in stability and can react sensitively and quickly with cyanide ions in a water environment, cyanide ions in the water environment can be detected quickly and sensitively by using both ultraviolet spectrophotometry and two-photon excited fluorescence method, and lowest detection limit of ultraviolet spectrophotometry is up to 0.5 MuM, with lowest detection limit of the two-photon excited fluorescence method up to 0.01 MuM.

The invention discloses a method for testing quality of reineckea carnea medicinal materials, which includes part or whole of description, identification, examination and content measurement. The aforementioned is indicated as a thin-layer chromatography identification of kitigenin and/or kitigenin 5-O-beta-D-glucopyranose saponin, the content measuring is indicated as measuring the content of saponin in medicinal materials with the method of ultraviolet spectrophotometry. Compared to the existing technology, the invention brings forward a reineckea carnea thin-layer chromatography identification process and total saponin content measuring process with taking kitigenin and kitigenin 5-O-beta-D-glucopyranose saponin as reference substance, which perfects the quality detecting standard of reineckea carnea medicinal materials, recuperates the deficiency of the existing quality detecting technology, makes this quality detecting technology more scientific and reasonable, and ensures its security and validity in clinical application.

The invention discloses a method for quickly measuring the nitrate content in aquatic products, which comprises: measuring the nitrate content by using gradient restoration ultraviolet spectrophotometry according to the characteristics of the K value of the characteristic peaks of solution of nitrate, ashing weighed aquatic products to be measured, and preparing solution A and solution B, wherein the concentration of the solution A is twice that of the solution B; measuring the absorbancy value of the solution A and the solution B to be measured by using ultraviolet spectrophotometry respectively, calibrating the absorbancy value of the solution B at a corresponding position, reading the value of a corresponding concentration C1, recording the value as a one-time point, marking the absorbancy value of the solution A at the position of a concentration C2 which is twice the concentration C1, marking as a double point, and obtaining a measured K* value; and comparing with the K value of a standard curve to obtain the nitrate concentrations in the measured solutions. The method is simple, visual, quick, reliable and accurate, the detection limit of the method is 1 mu g/ml, and ionic interference is prevented. The method disclosed by the invention is used to measure the nitrate content in aquatic products and also can be used for quick measurement of other soluble salts having characteristic absorption peaks.

The invention discloses a method for measuring content of ascorbic acid in plants by utilizing ultraviolet spectroscopy. The method comprises the following steps of: measuring an ultraviolet absorption value of the ascorbic acid in sample extraction buffer, damaging the ascorbic acid so as to measure a background ultraviolet absorption value through an alkali treatment method, and calculating the content of the ascorbic acid through a difference. The method is characterized in that: a high-concentration alkali treatment method is adopted when a background sample is measured, so that the reduced form ascorbic acid is eliminated completely, and the influence of an alkaline environment on ultraviolet absorption of solution to be measured is avoided simultaneously. The method is simple to operate, the measurement result is accurate, and the reducing substances are not easy to get disturbed. The method is suitable for measuring sample ascorbic acid with higher numbers in botanical studies.

The invention discloses a spectroscopic seawater nitrate concentration detection method which comprises the following steps: 1) measuring a seawater spectrum by using an ultraviolet spectrophotometry,and calculating absorbance to obtain an ultraviolet absorption spectrum of seawater; 2) carrying out smoothing pretreatment on the seawater ultraviolet absorption spectrum; 3) sequentially carrying out seawater chloride ion, bromide ion and turbidity interference deduction on the absorption spectrum; and 4) establishing a partial least squares model by utilizing the absorption spectrum subjectedto smooth pretreatment and interference deduction, and optimizing selection of a modeling waveband to obtain a seawater nitrate concentration partial least squares method final meter. The spectroscopic seawater nitrate concentration detection method provided by the invention can effectively reduce errors caused by random noise when the seawater nitrate concentration is measured by the spectrography, can reduce interference caused by other substances with absorption to an ultraviolet band in seawater, and can improve the nitrate concentration prediction precision.

The invention discloses a method for detection of biological activity of the Fc region of human immune globulin through ultraviolet spectrophotometry. According to the method, an adsorbed diphtheria-acellular pertussis-tetanus vaccine is used to substitute high-cost hardly-available labelled antigen for sensitization erythrocytes; an Ultrospec 6300pro ultraviolet/visible light spectrophotometer produced by GE Company is used for detection of a sample, and software of the spectrophotometer carries out data processing. Experimental results of the invention prove that standard deviation is in a range of 0.06 to 15.92% and variance is in a range of 0.00 to 1.69%. Results of comparison between the results of an ultraviolet spectrophotometer and the mean value of three detection results of the method provided by the invention are as follows: average data deviation is -1.37%, standard deviation of data difference values is -1.28%, and variance of data difference values is 0.02%. The detection results of the invention is in a reasonable theoretical scope, so the method provided by the invention is indeed a cheap method for micro-scale detection of biological activity of the Fc region of human immune globulin in a blood product.

The invention relates to a high porosity cystose MnO2 catalytic material and a preparation and the application thereof, solving problems of catalyzing highly efficiently, prolonging the service life of catalyzed matters, improving the purity of the catalyzed matters, improving the catalytic effect and the like while ozone flows through a carrier. The catalytic material can be applied to an ozone converter of an ultraviolet spectrophotometry ozone analyzer based on a microprocessor and used as a highly efficient catalytic active matter for alkaline manganese batteries. Foam sponge is used as base material. After the conductive treatment, the foam sponge is plated with copper on the surface firstly. When Cu plated film reaches the required thickness, the foam sponge is taken out to be dried. The heat treatment is implemented on the foam sponge in a vacuum furnace or a furnace which is protected with argon. A cystose copper mesh is molded. Then, an Mn plated layer is deposited on the surface of the cystose copper mesh. When the Mn plated layer reaches the required thickness, the mesh is taken out and dried. Under the common oxygen atmosphere of an atmospheric pressure, the oxidation treatment is implemented on the copper mesh to obtain the high porosity cystose MnO2 catalytic material. The porosity of the material is 85-95 percent, and the specific area is regulated in the scope of 12.6m<2>/m<3>-29.6m<2>/m<3> according to practical requirements.

The invention discloses a method and a system used for measuring influence contribution rate of forest litter on surface runoff element contents. The method comprises following steps: firstly, the system used for measuring influence contribution rate of forest litter on surface runoff element contents is constructed; runoff plots are uniformly covered with forest litter using grid method; artificial simulated rainfall or natural rainfall are adopted so as to carry out experiments; after starting of rainfall, standard rain gauges and collecting tanks are used for collecting rainfall and runoff synchronously, and collecting samples; the rainfall and runoff samples are subjected to pre-treatment so as to remove impurities; ultraviolet spectrophotometry and platinum antimony colorimetry are adopted to measure the total nitrogen concentration content and the total phosphor concentration content in the rainfall and runoff samples respectively. The method and the system are capable of separating influences of forest surface soil on surface runoff chemical elements such as N and P, and measuring influence contribution rate of forest litter on the contents of surface runoff chemical elements such as N and P effectively at the same time.

The invention discloses a degrading bacterium for autotoxic substances of the rhizosphere of an apple tree and an application of the degrading bacterium. The strain is sorted and named Azospirillum lipoferum BL2; the collection unit is General Microbiological Center of China Committee for Culture Collection of Microorganisms (CGMCC); the address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; the collection date is 28th August, 2014; and the collection number is CGMCC No.9619. The strain has good degrading effect on phlorizin, p-hydroxybenzoic acid, phthalic acid and pyrogallic acid respectively under the condition of liquid culture in a shake flask. After the strain is subjected to liquid shake culture for three days, the result determined by an ultraviolet spectrophotometry shows that the degrading rates of the phlorizin, p-hydroxybenzoic acid, phthalic acid and pyrogallic acid are as follows respectively: 36%, 34%, 31% and 65%. The result shows that the BL2 strain has good degrading effect respectively on four types of autotoxic substances, and has potential application value for relieving successive cropping obstacles caused by the autotoxic substances.

The invention discloses a detecting method for hexavalent chromium in gelatin and products thereof, particularly relates to the detecting method for the hexavalent chromium in colored gelatin products. The method includes the following steps: using phosphate buffered solution to extract; adjusting PH value of the extraction solution to >=12.5; directly adding graphitized carbon black powder in the extraction solution with colorant for purification and decoloration; in acid medium, hexavalent chromium oxidation diphenyl carbonyl two hydrazine forms soluble amaranthine compounds. The ultraviolet spectrophotometry method is adopted to detect the content of the hexavalent chromium. The detecting method has the advantages that under the condition of highly alkalinity, the recovery rate of the hexavalent chromium can be guaranteed. Moreover, the graphitized carbon black powder can carry out sufficient decoloration for pigment in samples and avoid color interference. The detecting method is high in recovery rate, simple in pretreatment, low in cost, quick and accurate in result.

The invention provides a detection method for the concentration of a demulsifying agent in crude oil. The detection method comprises the following steps: (1) preparing a standard solution with a gradient concentration by utilizing the demulsifying agent; adding an extracting agent and extracting; developing through a color developing agent; determining the absorbance of an organic layer of the standard solution through ultraviolet spectrophotometry; drawing a standard curve according to the absorbance and the concentration; (2) adding the demulsifying agent with the known content into a sampleto be detected; separating an oil phase and a water phase in the sample to be detected, so as to obtain a water phase layer and a crude oil layer; (3) adding the extracting agent into the water phaselayer to extract and adding the color developing agent to carry out color development; determining the absorbance of the organic layer of the sample to be detected through the ultraviolet spectrophotometry; quantifying the concentration of the demulsifying agent in the water phase layer in step (2) according to the standard curve drawn in step (1); and (4) obtaining the concentration of the demulsifying agent in the crude oil according to the concentration of the demulsifying agent in the water phase layer in step (3) and the demulsifying agent with the known content in step (2).

The invention relates to a method for measuring index component content in a Qingkailing injection midbody and a finish product. The invention is characterized in that each index component content is measured by adopting the high performance liquid chromatography or other analysis methods; the diluted sample is scanned in full wavelength by adopting the ultraviolet spectrophotometric method; the sample is divided into two parts: a corrected collection and a verified collection. According to the ultraviolet spectroscopic data and the content of the corrected collection, the data are processed by adopting the chemometrics method; a mathematical model is established by utilizing a TQ Analyst software and a Matlab 6.5 software; the ultraviolet spectroscopic data of the verified collection sample is substituted into the mathematical model; a predicted value and a true value are compared, and the precision and the reliability of the technology are verified. The method of the invention can be used for content measurement of chlorogenic acid and baicalin in the silver yellow liquid, and the content measurement of Caryptoside and total nitrogen in the four-miscible liquid. The invention also can be used for the fast online detection of component content hereinbefore, and can be used for the content measurement and fast online detection of the Qingkailing injection midbody and the finish product.

The invention discloses a nano copper oxide with the activity of an ascorbic acid oxidization mimic enzyme, and aims at effectively catalyzing ascorbic acid oxidization to generate dehydroascorbic acid under aerobic environment and keeping the activity of the ascorbic acid oxidization mimic enzyme under strong acid, strong base or high temperature. By utilizing an ultraviolet spectrophotometry, the influences, on the nano copper oxide as ascorbic acid oxidase, of the temperature, pH and reaction time are respectively researched. The steady-state kinetic parameters of the nano copper oxide as the ascorbic acid oxidase are equivalent to the natural ascorbic acid oxidase, and the stability of the nano copper oxide as the ascorbic acid oxidase is obviously stronger than the natural ascorbic acid oxidase. By utilizing a deoxidization experiment and a fluorescence analysis method, a mechanism of the nano copper oxide as the ascorbic acid oxidase to catalyze ascorbic acid oxidization is verified. The activity of the ascorbic acid oxidization mimic enzyme shows a relatively tempting application prospect in the technical fields of nano enzymes, nanometers and bionic.

The invention discloses a pediatric paracetamol granule and a quality control method. The formulation is as follows: acetaminophen: in-vitro-cultured calculus bovis: chlorpheniramine maleate (125:5:0.5); the quality control method which combines a character method, thin-layer chromatography, thin layer chromatography scanning, and ultraviolet spectrophotometry is adopted; in the invention, in-vitro-cultured calculus bovis replaces natural calculus bovis to be used for the pediatric paracetamol granule, thus greatly reducing raw material cost of medicines while guaranteeing drug effect; in addition, in-vitro-cultured calculus bovis is used for replacing natural calculus bovis to be used for pediatric paracetamol granule, thus solving medicinal safety and drug effect problem, and simultaneously being beneficial to roundly controlling the quality of the pediatric paracetamol granules.

The invention relates to the field of medical testing and discloses a kit for detection of 1,5-AG and a method thereof. According to the kit, by selection of appropriate reagent R1 and reagent R2 andby adoption of HK+G6PD method+ lactate dehydrogenase method, endogenous glucose in a 1,5-AG sample is effectively eliminated, and then the content of 1,5-AG in human serum is detected by the utilization of an oxidase-ultraviolet spectrophotometry method. The test results show that the kit provided by the invention can eliminate the interference of 1-20nM endogenous glucose. In comparison with a detection kit in the prior art, the kit of the invention has advantages of wider application range, high test result accuracy, good precision and wide linear range, and can be widely used for measuringthe content of 1,5-AG in serum in hospitals of different levels, health and prevention departments and research and development institutions of medical biology.

The invention relates to a shuteria involucrata root extract as well as a preparation method and application thereof and belongs to the field of modernization of Chinese traditional medicines. The shuteria involucrata root extract is prepared by the following steps: taking shuteria involucrata roots as raw materials, carrying out ethanol water extraction and recycling a solvent to obtain a total extract; after water dispersion, centrifuging the total extract; degreasing the centrifuged liquid with petroleum ether and then extracting with ethyl acetate; recycling an extracting agent to obtain an ethyl acetate extract; after diluting the extract, carrying out column chromatography separation; determining and collecting a flavone part through a thin layer chromatography analysis and ultraviolet spectrophotometry, and concentrating the flavone part to obtain the extract. The research finds that the shuteria involucrata root extract has remarkable activity for relieving a cough and eliminating phlegm and particularly has an excellent phlegm eliminating effect when the general flavone part is in a low dosage; the shuteria involucrata root extract is safe and effective and can be used for preparing medicines for preventing and treating a cold and chronic bronchitis.