Pesticide residue rapid detection method based on nanogold model enzyme

A technology for pesticide residues and detection methods, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problem of harsh reaction conditions, high cost, and difficulty in natural enzyme modification. Preservation and other issues, to achieve the effect of low environmental conditions, low cost, and good stability

Inactive Publication Date: 2018-01-19
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Related invention patents include "a nanomaterial for detecting dimethoate and its preparation method", application number: 201410051064.4, which discloses using sol-gel as a carrier, immobilizing plant acylase molecules in the mesoporous channels of the carrier, and then Prepare J-aggregates on the surface of immobilized plant acetylase, and finally separate and dry to obtain detection nanomaterials, but the natural enzyme modifiers used in it require harsh reaction conditions and are not easy to store
Related invention patent "a reagent for colorimetric detection of omethoate based on nucleic acid aptamer-coated AuNPs probe and its application", application number 201510263644.4, the AuNPs in the disclosed detection method need to be modified to the sequence: 5'-AGCTTGCAGCGATTCTTGATCGCCACAGAGCT -3'nucleic acid aptamer, the preparation process requires high conditions and high cost
The complex modification process of AuNPs in these methods limits their applications to some extent.

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  • Pesticide residue rapid detection method based on nanogold model enzyme
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  • Pesticide residue rapid detection method based on nanogold model enzyme

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Embodiment 1

[0039] 1. Preparation method of AuNPs:

[0040] (1) Preparation method of seed solution: Add 5 mL of HAuCl with a concentration of 0.5 mM to 5 mL of CTAB solution with a concentration of 0.2 mol / L in a constant temperature water bath at 30 °C 4 Aqueous solution, after stirring and mixing, add 0.6mL sodium borohydride solution with a concentration of 0.01M and place it in an ice-water bath. A gold seed sol containing gold nanocrystals.

[0041] (2) Preparation of AuNPs: 150 μL of 4 mM silver nitrate solution and 5 mL of 0.5 mM HAuCl were added to 5 mL of 0.2 M CTAB solution 4 Solution, after stirring evenly, slowly add 70 μL of vitamin C with a concentration of 78.8 mM, vitamin C will immediately reduce the solution from brown to colorless, and stir the solution at 700 r / min for 2 minutes to obtain a growth solution; add 12 μL of gold crystal seed sol Stir in 10mL of growth solution at 200r / min for 3min, the solution turns from colorless to purple blue, place the solution at ...

Embodiment 2

[0057] 1. The preparation method of AuNPs: with specific embodiment 1.

[0058] 2. Establish a standard curve for organophosphorus pesticides (OPs) dichlorvos:

[0059] (1) Dichlorvos standard solution preparation: Dilute the dichlorvos standard with distilled water to a series of concentrations of 0 μL / mL, 20 μL / mL, 40 μL / mL, 60 μL / mL, 80 μL / mL, and 100 μL / mL;

[0060] (2) Preparation of chromogenic AB solution: preparation of solution A: take 0.012g TMB and dilute it with absolute ethanol to a 10mL volumetric flask, and ultrasonically oscillate for 3 minutes to obtain a TMB solution with a concentration of 1mM; preparation of solution B: take 0.575gH 2 o 2 Add it into a 10mL volumetric flask, make it up to volume with distilled water, shake evenly to get a concentration of 10mM H 2 o 2 Solution; AB solution needs to react within 2 hours after preparation.

[0061] (3) To establish a standard curve: take 40 μL of dichlorvos with a concentration of 0 μL / mL, 20 μL / mL, 40 μL...

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Abstract

The invention provides an OPs rapid detection method based on AuNPs mimetic peroxidase. The method includes the following three steps: 1, preparation of AuNPs mimetic peroxidase; 2, establishment of an organophosphorus pesticide OPs standard curve; and 3, detection of the content of OPs in a sample. The invention is a is convenient, rapid and visual colorimetric pesticide detection method based onsingle AuNPs as the model enzyme, the used nanogold can be prepared directly, no further modification is needed, and the preparation is simple and fast; the detection has low requirements for environmental conditions, and can adapt to wide temperature and PH conditions; the detection steps are simple, complex and expensive equipment are not involved, and the cost is low; the detection sensitivityis high, and the minimum detectable limit of ultraviolet spectrophotometry can reach 1.73; and the stabilizer CTAB is added in the preparation process of the AuNPs model enzyme solution, precipitatedoes not form easily, the stability is good, and the storage time is long.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, in particular to a rapid detection method for pesticide residues based on gold nanometers (AuNPs) simulated enzymes, and relates to the preparation of peroxide simulated enzymes based on gold nanoparticles (AuNPs) and its use in the rapid detection of organophosphorus pesticides (OPs). test methods. Background technique [0002] In recent years, the use of OPs has always been the first among all kinds of pesticides, and it is the most widely used and the largest amount of pesticides in my country. OPs mainly include dichlorvos, parathion (1605), phorate (3911), demeton (1059), dimethoate, trichlorfon, malathion (4049), acephate, etc. [0003] Trace amounts of OPs can cause acute and chronic poisoning in humans, and most of the pesticides used in agricultural production have entered the natural environment and caused serious ecological pollution; therefore, the content of OPs in natur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 邹小波石海军石吉勇黄晓玮翟晓东蒋彩萍李志华胡雪桃徐艺伟
Owner JIANGSU UNIV
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