Quality detection method for tibetan medicine rhododendron anthopogonoide and tibetan medicine rhododendron anthopogonoide preparation

A quality detection method, the technology of the rhododendron, which is applied in the direction of measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems that cannot fully reflect the quality of the rhododendron

Active Publication Date: 2012-12-05
SHANDONG JINHE DRUG RES DEV
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  • Abstract
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Problems solved by technology

This method is used to control the quality of Rhododendron lanceolata through the content determin...

Method used

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  • Quality detection method for tibetan medicine rhododendron anthopogonoide and tibetan medicine rhododendron anthopogonoide preparation
  • Quality detection method for tibetan medicine rhododendron anthopogonoide and tibetan medicine rhododendron anthopogonoide preparation
  • Quality detection method for tibetan medicine rhododendron anthopogonoide and tibetan medicine rhododendron anthopogonoide preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0075] Experimental example 1: Determination experiment of total flavonoids in Rhododendron lanceolata

[0076]1. Instruments, reagents and test samples

[0077] Instruments: Shimadzu UV-2450 ultraviolet-visible spectrophotometer; Shimadzu AuW220D electronic balance.

[0078] Reference substance: hyperoside reference substance (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 111521-201004.

[0079] Sample: Rhododendron japonica (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers: 110901, 110902, 110903, 111101, 111102, 111103.

[0080] 2. Preparation of reference solution

[0081] Take 15mg of hyperoside reference substance, weigh it accurately, put it in a 25ml measuring bottle, add an appropriate amount of ethanol, put it on a water bath to dissolve it, let it cool, add ethanol to the scale, and shake well; take 10ml precisely, and put it in 100ml In the bottle, add water to the mark and shake well to obtain (...

experiment example 2

[0106] Experimental example 2: Determination experiment of total quercetin and kaempferin in rhododendron

[0107] 1. Instruments, reagents and test samples

[0108] Instrument: High performance liquid chromatography: Hitachi L-2100 pump, Hitachi L-2400 UV detector;

[0109] Electronic balance: Shimadzu AUW220D type.

[0110] Reference substance: quercetin reference substance (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 100081-200406; kaempferol (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 110861~200808.

[0111] Sample: Rhododendron japonica (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers: 110901, 110902, 110903.

[0112] 2. Detection wavelength selection

[0113] Take quercetin and kaempferol mixed reference solution, scan in the wavelength range of 190-400nm, and select 360nm as the detection wavelength according to the ultraviolet absorption spectrum. ...

experiment example 3

[0165] Experimental example 3: Establishment of fingerprints of flavonoids in Rhododendron lanceolata

[0166] 1. Instruments, reagents and test samples

[0167] Instrument: High performance liquid chromatography: Hitachi L-2100 pump, Hitachi L-2400 UV detector;

[0168] Electronic balance: Shimadzu AUW220D type.

[0169] Reference substances: quercetin reference substance (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 100081-200406; kaempferol (National Institute for the Control of Pharmaceutical and Biological Products), batch number: 110861~200808; National Institute for the Control of Pharmaceutical Products), batch number: 111521-200303; rutin (China Institute for the Control of Pharmaceutical and Biological Products), batch number: 10080-200306.

[0170] Sample: Rhododendron japonica (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers: 110901, 110902, 110903.

[0171] 2. Detection wavelength selection

...

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Abstract

The invention provides a quality detection method for tibetan medicine rhododendron anthopogonoide and a tibetan medicine rhododendron anthopogonoide preparation. The method includes: determining the total flavonoid content in the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation by ultraviolet-spectrophotometry; simultaneously determining the total quercetin content in the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation in the same chromatograph by high-performance liquid chromatography; and establishing a finger-print chromatogram of flavonoids in the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation by the high-performance liquid chromatography. Therefore, quality of the rhododendron anthopogonoide and the rhododendron anthopogonoide preparation can be guaranteed by detecting whether common peaks exist in the finger-print chromatogram or not, and further medication safety for patients is guaranteed. The quality detection method is stable in sample solution, high in precision and excellent in reproducibility, has certain specificity, is good in separating effect of each characteristic constituent in the finger-print chromatogram, can be used for quality detection of rhododendron anthopogonoide medical materials, and is beneficial to guarantee of safety, effectiveness, controllability in quality and good clinical effect for the tibetan medicine rhododendron anthopogonoide and the tibetan medicine rhododendron anthopogonoide preparation.

Description

technical field [0001] The invention relates to a quality detection method, in particular to a quality detection method of a Tibetan medicine rhododendron azalea and a preparation thereof. Background technique [0002] Rhododendron anthopogoniodes Maxim.et Franch is the dried flower and leaves or twigs with leaves of Rhododendron anthopogoniodes Maxim.et Franch. It is mainly used for bronchitis, emphysema, edema, physical weakness and soil discomfort, indigestion, gastroptosis, gastric dilatation, external use to treat sores. "Yuewang Medicine Clinic" records: "Treating dragons, Chiba, Bacon's disease, spleen and stomach deficiency and cold, indigestion, and acclimatization." "Qi Tie Garland" records: bitter taste, warm nature, light effect. Cure bacon's disease. "Mingshi Chapter Thirty" records: warm in nature, cure Bacon's disease, lung disease pain, hiccups. "Picture Book" records: This product was born in the shade of the mountain. The trunk is white, the leaves are...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N30/88
Inventor 张义智江玉娟孙绪丁刘玉芹任松鹏
Owner SHANDONG JINHE DRUG RES DEV
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