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Degrading bacterium for autotoxic substances of rhizosphere of apple tree and application of degrading bacterium

A technology of self-toxic substances and degrading bacteria, applied in the fields of applications, bacteria, microorganisms, etc., can solve problems such as degradation that has not been reported

Active Publication Date: 2015-01-28
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the degradation of various autotoxic substances by apple rhizobacteria

Method used

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  • Degrading bacterium for autotoxic substances of rhizosphere of apple tree and application of degrading bacterium
  • Degrading bacterium for autotoxic substances of rhizosphere of apple tree and application of degrading bacterium
  • Degrading bacterium for autotoxic substances of rhizosphere of apple tree and application of degrading bacterium

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with embodiment.

[0017] An apple rhizosphere autotoxic substance-degrading bacterium, the strain is classified and named as Azospirillum lipoferum BL2, preserved by: General Microorganism Center (CGMCC), China Committee for the Collection of Microbial Cultures, address: Chaoyang District, Beijing No. 3, No. 1 Yard, Beichen West Road, date of deposit: August 28, 2014, deposit number: CGMCC No.9619.

[0018] (1) Screening of CGMCC 9619

[0019] The process of enriching degrading bacteria with phthalic acid as the only carbon source: Take 10 g of apple rhizosphere soil samples from Xibaizipo Village, Beijipo Town, Tai’an, Shandong Province (N36°04′54.9″E117°08′51.3″) 90mL basal salt medium (NH 4 Cl 0.5g; NaCl 1g; K 2 HPO 4 ·3H 2 O 1.3g; MgSO 4 ·7H 2 (00.4g; distilled water 1000mL; pH7.2), 30°C, 180r / min shaker culture after 7d, with 5% inoculum size transferred to new containing phthalic acid bas...

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Abstract

The invention discloses a degrading bacterium for autotoxic substances of the rhizosphere of an apple tree and an application of the degrading bacterium. The strain is sorted and named Azospirillum lipoferum BL2; the collection unit is General Microbiological Center of China Committee for Culture Collection of Microorganisms (CGMCC); the address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; the collection date is 28th August, 2014; and the collection number is CGMCC No.9619. The strain has good degrading effect on phlorizin, p-hydroxybenzoic acid, phthalic acid and pyrogallic acid respectively under the condition of liquid culture in a shake flask. After the strain is subjected to liquid shake culture for three days, the result determined by an ultraviolet spectrophotometry shows that the degrading rates of the phlorizin, p-hydroxybenzoic acid, phthalic acid and pyrogallic acid are as follows respectively: 36%, 34%, 31% and 65%. The result shows that the BL2 strain has good degrading effect respectively on four types of autotoxic substances, and has potential application value for relieving successive cropping obstacles caused by the autotoxic substances.

Description

technical field [0001] The invention relates to an apple rhizosphere autotoxic substance degrading bacterium and application thereof. Background technique [0002] Apple (Malus pumila) is a plant of the Rosaceae (Rosaceae) genus pomaceous, a deciduous tree with oval leaves and white flowers with red halos. The fruit is round, sweet or slightly sour. It is a common fruit, rich in nutrients, and has the functions of dietotherapy and auxiliary treatment. It is one of the fruits widely accepted and loved by the public. In recent years, continuous cropping obstacles caused by continuous planting in major apple producing areas have become key technical issues restricting apple production. The main causes of apple continuous cropping obstacles include the deterioration of soil physical and chemical properties, the aggravation of soil-borne diseases and insect pests, and the autotoxic effect of autotoxic substances. Among them, the poisonous effect of autotoxic substances on plants...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/00A01G7/06C12R1/01C09K101/00
CPCA01G7/06C09K17/00C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 丁延芹毛志泉杜秉海祁国振刘凯姚良同
Owner SHANDONG AGRICULTURAL UNIVERSITY
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