Shuteria involucrata root extract as well as preparation method and application thereof

A root extract and extract technology, applied in the field of bean root extracts, can solve problems such as no research reports on the extracts of flavonoids from bean roots, and achieve significant antitussive and expectorant activity, high purity, A stable effect

Inactive Publication Date: 2016-03-09
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the flavonoids extract from bean root

Method used

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  • Shuteria involucrata root extract as well as preparation method and application thereof
  • Shuteria involucrata root extract as well as preparation method and application thereof
  • Shuteria involucrata root extract as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of perennial bean root extract

[0032] 1. Take 102 g of bean root and pulverize it until the particle size is 40 mesh;

[0033] 2. The bean root powder was reflux extracted with 1 L of 70% (v / v) ethanol aqueous solution for 3 times at a temperature of 65° C. for 2 hours each time. The three extracts were combined, and the extraction solvent was recovered to obtain 13.3 g of the total extract;

[0034] 3. Disperse the total extract with 500ml of water, centrifuge at 20,000 rpm with a tube centrifuge to obtain 420ml of centrifugate, degrease 2 times with 1.2L petroleum ether, and then extract 3 times with 1L ethyl acetate. The ethyl acetate layers were combined, and the extractant was recovered to obtain 8.16 g of ethyl acetate extract;

[0035] 4. The obtained ethyl acetate extract was diluted with 150ml of water to a concentration of 5% by mass. Take 400g of polyanionic cellulose and pressurize it into a column, equilibrate the column wi...

Embodiment 2

[0037] Embodiment 2: the preparation of perennial bean root extract

[0038] 1. Take 94g of bean root and pulverize it until the particle size is 40 mesh;

[0039] 2. Cold soaking and extracting 1 L of 80% ethanol aqueous solution for 3 times with 1 L of 80% ethanol aqueous solution, 24 hours each time, combining the extracts three times, recovering the extraction solvent to obtain 11.2 g of the total extract;

[0040] 3. Disperse the total extract with 500ml of water, centrifuge at 20,000 rpm in a tubular centrifuge to obtain 430ml of centrifugate, degrease 2 times with 1.2L petroleum ether, and then extract 3 times with 1L ethyl acetate. The ethyl acetate layers were combined, and the extractant was recovered to obtain 6.52 g of ethyl acetate extract;

[0041] 4. The obtained ethyl acetate extract was diluted with 110 ml of water to a concentration of 6% by mass. Take 400g of polyanionic cellulose and pressurize it into a column, equilibrate the column with methanol aqueou...

Embodiment 3

[0044] 1. Take 100 g of dried bean root and grind it to a particle size of 60 mesh;

[0045] 2. The bean root powder was reflux extracted with 0.5 L of 90% v / v ethanol aqueous solution for 4 times at a temperature of 70°C for 1 hour each time, the extracts were combined for 4 times, and the extraction solvent was recovered to obtain 12.3 g of the total extract;

[0046] 3. Disperse the total extract with 500ml of water, centrifuge at 20,000 rpm with a tube centrifuge to obtain a centrifuge, degrease the centrifuge with 1.2L petroleum ether 3 times, then extract 4 times with 1L ethyl acetate, and combine Ethyl acetate layer, recovery of extractant to obtain ethyl acetate extract 7.77g;

[0047]4. The obtained ethyl acetate extract is diluted with 400ml of water to a concentration of 2% by mass. Take polyanionic cellulose with 30 times the amount of extract and pack it into a column under pressure, equilibrate the column with methanol aqueous solution with a concentration of 35...

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Abstract

The invention relates to a shuteria involucrata root extract as well as a preparation method and application thereof and belongs to the field of modernization of Chinese traditional medicines. The shuteria involucrata root extract is prepared by the following steps: taking shuteria involucrata roots as raw materials, carrying out ethanol water extraction and recycling a solvent to obtain a total extract; after water dispersion, centrifuging the total extract; degreasing the centrifuged liquid with petroleum ether and then extracting with ethyl acetate; recycling an extracting agent to obtain an ethyl acetate extract; after diluting the extract, carrying out column chromatography separation; determining and collecting a flavone part through a thin layer chromatography analysis and ultraviolet spectrophotometry, and concentrating the flavone part to obtain the extract. The research finds that the shuteria involucrata root extract has remarkable activity for relieving a cough and eliminating phlegm and particularly has an excellent phlegm eliminating effect when the general flavone part is in a low dosage; the shuteria involucrata root extract is safe and effective and can be used for preparing medicines for preventing and treating a cold and chronic bronchitis.

Description

technical field [0001] The invention relates to an extract of bean root, its preparation method and application, and belongs to the field of traditional Chinese medicine modernization. Background technique [0002] The root of the bean is derived from the plant of the genus Fabaceae, the plant of the genus Fabaceae, Shuteria sinensis Hemsl, also known as copper coin ephedra and wild pea. It is a traditional national medicine of the Dai nationality. The medicine is bitter in taste and cool in nature. It enters the earth pagoda and has the effects of clearing fire, detoxifying, expelling wind and relieving pain. The plant is mainly distributed in central and southern Yunnan and Guangxi, and it is also distributed in Vietnam, Cambodia, Thailand, Indonesia, Nepal and other places. [0003] Chinese Subaodou is also a common medicinal material of many ethnic minorities such as Yi and Wa. The Yi medicine is called Yesuonu, which is called Yiyancao in the Yi people's prescription;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P11/00A61P11/14A61P11/10
Inventor 杨竹雅张宝洪谭文红张彬若马晓霞钱子刚杨树德
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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