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Method for extracting protein in biological sludge

A technology of biological sludge and extraction method, applied in sludge treatment, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the problems of black and sticky extraction liquid, excessive heavy metal ions, and heavy metal dissolution.

Inactive Publication Date: 2013-03-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is beneficial to the lysis of sludge and the release of protein in it, but the biggest problem with this method is: there is a relatively serious Medela reaction, which produces more side reactions, and the color of the extract is black and sticky. It is not conducive to the separation of proteins, and its protein quality is poor
However, the traditional acid direct extraction method has the problem of heavy metal dissolution, which can easily cause heavy metal ions in protein products to exceed the standard

Method used

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  • Method for extracting protein in biological sludge
  • Method for extracting protein in biological sludge
  • Method for extracting protein in biological sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 500g of dewatered sludge from a sewage plant (with 80% moisture content), add 1750g of distilled water and 250g of concentrated hydrochloric acid, then stir at room temperature for 30 minutes, and perform centrifugal filtration to filter the filtrate to a moisture content of 92%, and then put the mud Put it into the container and put it into the high-temperature digestion device. Heat for 2h at 120°C. After cooling, perform centrifugal filtration to obtain about 450ml of filtrate. Take out 60ml of filtrate (the rest of the filtrate is stored in the refrigerator). Put it into a 50ml small beaker, adjust a series of pH with sodium hydroxide solution, respectively, to be 3.0, 4.2, 5.2, 5.6, 6.0 and 7.0, put it at room temperature for 12 hours, then perform high-speed centrifugation, save the supernatant, and determine the corresponding The pH of the supernatant was 2.9, 3.6, 4.7, 5.3, 5.6, and 6.5, and then the protein content in the supernatant was measured by the Feli...

Embodiment 2

[0029] Same as Example 1, except that the heating time is 5h and 8h, and there is no need to determine the protein isoelectric point. The obtained filtrate is directly subjected to protein precipitation at the isoelectric point determined in Example 1, and then washed with 95% ethanol , And dried, the solid materials obtained were 14.43g and 16.76g, respectively. Its protein content is 102.1% and 99.8% respectively. Corresponding heavy metals chromium, cadmium, mercury and arsenic were not detected, and the mass content of lead (calculated as PbO) was 0.02% and 0.03% respectively, which met the quality standard requirements for protein feed.

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Abstract

The invention discloses a method for extracting protein in biological sludge. The method mainly comprises the steps of: sludge acid-washing, high-temperature acid lysis, isoelectric point determination, isoelectric point crystallization, organic solvent washing and low-temperature drying. A protein solid obtained by separation is fine light yellow powder, and the purity of the protein, which is monitored by adopting an ultraviolet spectrophotometry method with bovine serum albumin as a standard, can be more than 99.8 percent. The infrared spectroscopy of a product proves that the solid powder has remarkable protein structure characteristics. The X-ray fluorometric analysis of the product proves that the protein obtained by separation has no heavy metals of mercury, cadmium and chromium or a toxic element of arsenic and only contains a small amount of lead with the mass content of less than 0.03 percent in the terms of PbO, thereby according with the requirement of a protein feed for the index. A method for separating and recycling protein in sludge generated in the sewage biological treatment process is beneficial to recycling of municipal sludge.

Description

Technical field [0001] The invention relates to a method for extracting protein from biological sludge produced in the biological treatment of urban sewage, and belongs to the field of solid waste treatment and resource utilization. Background technique [0002] With the development of social economy and the acceleration of urbanization, the amount of urban sewage and sludge is increasing rapidly. my country’s "Eleventh Five-Year Plan" report clearly pointed out that the COD emission reduction of sewage discharge should be reduced by 10% on the basis of the "Tenth Five Year Plan". Therefore, in recent years, the number of sewage plants in our country has increased rapidly, and a large amount of biological sludge has followed. Biological sludge is a flocculent mixture composed of microorganisms as the main body and suspended solids and colloidal substances in sewage, which contains a large amount of protein resources. It is reported in the literature that the crude protein conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C02F11/00
Inventor 刘勇郭祥
Owner TIANJIN UNIV
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