Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum

A technology for quantitative determination and compound, applied in the rapid quantitative determination of triterpenoid content in Ganoderma lucidum, quantitative determination, ultraviolet spectrophotometry to the rapid field, can solve the problems of unworthy of the name, inability to detect and quantitative determination, to achieve low cost, shorten the Measurement time, easy and fast reproducibility

Active Publication Date: 2004-11-17
无锡市三联高科技开发有限公司
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  • Abstract
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Problems solved by technology

Since there is no simple and practical detection method for such active ingredients, it is impossible t...

Method used

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  • Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum
  • Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum
  • Method for rapidly and quantitatively determining the triterpenoid content in ganoderma lucidum

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1 Instruments and materials

[0030] 1.1 Instruments TU-1901 double-beam UV-Vis spectrophotometer; high pressure liquid chromatography (HP 1100series); ODS RP-C 18 Chromatographic column: 5μm filler, 250mm×4.6mm. Portable UV detection analyzer UV-III type (detection wavelength 254nm).

[0031] 1.2 Materials Ganoderma lucid um (Fr.) Karst fruiting body (produced in Tai'an, Shandong). Methanol and acetic acid are chromatographically pure reagents, and chloroform and sodium bicarbonate are analytically pure reagents. 0.45μm microporous membrane (domestic).

[0032] 2 Experimental methods

[0033] 2.1 Standard product preparation and detection

[0034] First extract the total triterpenoids from Ganoderma lucidum powder according to the literature method, and then use repeated silica gel column chromatography to separate, use chloroform:methanol=98:2, 95:5, 50:50 gradient elution, collect 98:2 wash The separated fractions were tested. Thin Layer Chromatography (TLC) d...

Embodiment 2

[0050] Repeat Example 1 according to the same steps as described, but the ratio of Ganoderma lucidum test product to chloroform is 1 (g): 20 (ml), after soaking in chloroform for 0.5h, centrifuge to obtain the supernatant, and use an equal amount of saturated NaHCO 3 The solution was extracted 3 times, and the extract III was collected, that is, NaHCO 3 Layer, the content of triterpenoids in 257nm detection. After three determinations, the average content of triterpenoids was 1.51%.

Embodiment 3

[0052] Repeat Example 1 according to the same steps as described, but the ratio of Ganoderma lucidum test product to chloroform is 1 (g): 10 (ml), after soaking in chloroform for 0.5h, centrifuge to obtain the supernatant, and use an equal amount of saturated NaHCO 3 The solution was extracted 3 times to collect NaHCO 3 Layer, 257nm detects the content of triterpenoids in the extract solution III. After three determinations, the average content of triterpenoids was 1.49%.

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Abstract

The invention is a method for measuring the content of triterpenes compound in Ganoderma Lucidum, especially a method for using ultraviolet splitting luminosity to measure, the invention refers to biology technology field. The technology project is: the Ganodeerma Lucidum is put into a cleaned tube, adds chloroform to immerse for 0.5-1 hours, and acquires the supernate after being centrifugated, uses the saturate NaHCO3 solvent to extract for 2-3 times, collects the NaHCO3 layer, acquires the liquid for test, measures the volume accurately, uses ultraviolet splitting luminosity method to measure the light absorbing valve under 250-260nm wavelength, works out the content of triterpenes in the sample according to the standard curve. The method saves the acidification, chloroform extraction, reduced pressure distillation and drying steps, the period of measurement is shortened greatly.

Description

1. Technical field [0001] The invention relates to a method for rapidly and quantitatively determining the content of triterpenoids in Ganoderma lucidum, in particular to a method for rapidly and quantitatively determining by ultraviolet spectrophotometry, and relates to the field of biotechnology. 2. Background technology [0002] The separation and purification of Ganoderma lucidum [Ganoderma lucidum (Fr.) Karst] polysaccharide components, the composition structure, pharmacology, immune and anti-tumor activity research and clinical application have been widely seen in the literature. For the separation and purification of triterpenoids, structure There are also some reports on identification and biological activity, and it has always been an important direction of research on Ganoderma lucidum in the world. Its biological activities include inhibiting tumor cell proliferation, anti-virus, antibacterial, analgesic, liver protection and so on. The extraction process of trite...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 杨新林黄书铭朱鹤孙徐建兰
Owner 无锡市三联高科技开发有限公司
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