Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry

A purple sweet potato anthocyanin and spectrophotometry technology, which is applied in the detection field, can solve problems such as being unsuitable for use in grassroots factories and requiring standard samples, and achieve the effects of low detection cost, simple calculation and obvious results.

Inactive Publication Date: 2011-04-20
湖北紫鑫生物科技有限公司
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  • Application Information

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Problems solved by technology

This method can reflect the total pigment content more accurately, but i

Method used

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  • Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry
  • Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry
  • Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry

Examples

Experimental program
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Effect test

experiment Embodiment 1

[0022] Experimental Example 1: Take 10ml of purple sweet potato anthocyanin pigment extract in 6 beakers, adjust the pH to 1, 2, 3, 4, 5, 6 with 0.1mol / L HCl and 0.1mol / L NaOH , and then transferred to a 100mL volumetric flask, and adjusted to 100ml with a buffer solution of disodium hydrogen phosphate-citric acid with a pH of 1, 2, 3, 4, 5, and 6, respectively. After equilibrating for 2 hours, distilled water was used as a blank sample for detection. Scan in the wavelength range of 400-600nm to obtain the maximum absorption wavelength and maximum absorption value at different pH values. Table 1 shows the absorption of purple sweet potato anthocyanins at different pH values ​​at the same concentration.

[0023] Table 1 Effect of pH value on absorbance and maximum absorption wavelength of purple sweet potato anthocyanin pigment

[0024]

[0025] It can be seen from Table 1 that as the pH value decreases, the absorbance of purple sweet potato anthocyanin pigments increases....

experiment Embodiment 2

[0026] Experimental Example 2: The prepared purple sweet potato anthocyanin pigment solution with a pH value of 3 was heated in a constant temperature water bath at different temperatures for 30 minutes, and its absorbance was measured at 528nm. It was found that the absorbance did not change significantly at 10-60°C, and was greater than 60 The absorbance decreases gradually at ℃. The experimental results show that with the increase of heating temperature and the extension of heating time, the stability of the pigment gradually decreases, and it has better stability under acidic conditions at room temperature. High temperature and long-term heating should be avoided during operation.

Embodiment 3

[0027] Embodiment 3: the mensuration of the maximum absorption wavelength of the sweet potato anthocyanin pigment in the b step solution is specifically to prepare a solution with a pH of 3 and a pigment concentration of 1%, and carry out ultraviolet scanning in the range of 400-600nm to obtain an absorption spectrum, such as figure 2 shown. from figure 2 It can be seen that when the pH value is equal to 3, the maximum absorption wavelength of the purple sweet potato anthocyanin pigment is 528±1nm.

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Abstract

The invention relates to a detection method, in particular to a method for detecting a purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry. The method comprises the following steps of: a, preparing purple sweet potato anthocyanidin pigment extract; b, adjusting the purple sweet potato anthocyanidin pigment extract to be acidic; and c, under the maximum absorption wavelength of the purple sweet potato anthocyanidin pigment, detecting the absorbance of anthocyanidin pigment solution to be detected. The detection method is easy to operate, convenient to compute, and accurate in result, has low cost of used instruments and low detection cost, and is suitable for the frontline of production; meanwhile, the detection method is special for the purple sweet potato anthocyanidin pigment, and the invention provides an economic, visual and rapid qualitative and quantitative test for purple sweet potato anthocyanidin pigment production enterprises.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting purple sweet potato anthocyanin pigment by ultraviolet-spectrophotometry. Background technique [0002] Natural purple sweet potato anthocyanin has many advantages: non-toxic, no side effects, high safety; natural color tone, close to natural substances; convenient source of raw materials, low price; simple process, low extraction cost; It has good stability in beverages and strong anti-lipid oxidation; it has various nutritional, pharmacological and health functions. Therefore, purple sweet potato anthocyanin pigments are widely used in food, chemical, cosmetic and pharmaceutical industries. At present, there are many studies on the extraction, purification and functional properties of purple sweet potato anthocyanin pigments, but less research on detection methods. The detection methods are generally extended to other pigment detection methods, and there is no unifi...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 周树勇
Owner 湖北紫鑫生物科技有限公司
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