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Method for determining uric acid content in poultry manure by adopting ultraviolet spectrophotometry

A technology of spectrophotometry and spectrophotometer, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as chromatographic column damage, cumbersome extraction methods, and unsatisfactory chromatographic peaks

Active Publication Date: 2019-11-05
HUAIYIN TEACHERS COLLEGE
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Problems solved by technology

[0005] The purpose of the present invention is to solve the traditional high performance liquid chromatography detection uric acid sample that exists in the prior art, the extraction method is loaded down with trivial details, the chromatographic peak is not ideal enough, mobile phase mostly uses phosphate, the shortcoming that damages the chromatographic column relatively, and proposes A method for the determination of uric acid in poultry feces by ultraviolet spectrophotometry

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  • Method for determining uric acid content in poultry manure by adopting ultraviolet spectrophotometry
  • Method for determining uric acid content in poultry manure by adopting ultraviolet spectrophotometry
  • Method for determining uric acid content in poultry manure by adopting ultraviolet spectrophotometry

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Embodiment Construction

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them.

[0019] In the present embodiment, the method for measuring uric acid content in poultry manure by ultraviolet spectrophotometry may further comprise the steps:

[0020] S1. Select 250 mg of feces raw material samples, dry them in the sun, remove impurities, crush them, and then filter them with a 40-mesh sieve;

[0021] S2, adding 100ml of glycine buffer solution with a pH value of 8.3 to the feces raw material to form a mixed sample for testing;

[0022] S3. Heat the sample for 20 min under the condition of heating in a water bath at 50° C. for hydrolysis;

[0023] S4. Dilute the hydrolyzed sample to 15 times the volume with 7.35% PCA, and then perform zero adjustment with PCA:water=1:1;

[0024] S5. Set the ultraviolet spectrophotomete...

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Abstract

The invention relates to the technical field of poultry fecal uric acid content, in particular to a method for determining the uric acid content in poultry manure by adopting ultraviolet spectrophotometry. The method includes the following steps of S1, selecting a fixed mass of a manure raw material sample, and conducting drying, impurity removal, crushing and filtering by means of a screen; S2, adding quantitative glycine buffer solution to the manure raw material to form a mixed sample for testing; S3, heating the mixed sample under the condition of heating in a water bath for 20 minutes for hydrolysis; S4, diluting the sample after hydrolysis to a volume of 15 times with 7.35% PCA, and then conducting zeroing according to a ratio of water to PCA of 1:1; S5, detecting the sample after zeroing by means of an ultraviolet spectrophotometer, and measuring the absorbance. The method, by adopting the ultraviolet spectrophotometry, has low requirements on equipment and testing personnel, also has high stability and repeatability, and thus can be used for qualitative measurement in first-line production.

Description

technical field [0001] The invention relates to the technical field of uric acid content in poultry feces, in particular to a method for measuring uric acid content in poultry feces by ultraviolet spectrophotometry. Background technique [0002] With the continuous improvement of people's living standards, people's demand for meat products, milk, and eggs has also shown an increasing trend year by year, which has led to the rapid development of animal husbandry, but at the same time it also produces a large amount of livestock and poultry manure , causing serious pollution. How to deal with livestock and poultry manure has become a major problem faced by major farms. [0003] Studies have shown that the protein content of poultry feces is 24%-30%. According to data, due to the short intestines of poultry animals, the digestion and absorption time of food in the intestines is short, and more than 60.0% of the nutrients in poultry feed are directly excreted through the intest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 谢鹏蔡羽娟陶德军徐永刚刁恩杰汪沐
Owner HUAIYIN TEACHERS COLLEGE
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