Multi-target simultaneous detection method based on concentration change of latex microspheres

Abstract

The invention belongs to the technical field of biochemical analysis. The invention particularly relates to a multi-target simultaneous detection method based on concentration change of latex microspheres. Nanometer magnetic particles are used as magnetic separation carriers, and the surfaces of the nanometer magnetic particles are coupled with capture antibodies of different target objects; latex microspheres with different particle sizes are used as signal probes, the surfaces of the latex microspheres are coupled with detection antibodies or complete antigens of different target objects, and each particle size corresponds to one target object; the magnetic separation carriers, the signal probes and a to-be-detected target object are subjected to immune reaction; after magnetic separation, a signal probe solution which does not participate in reaction in a separation solution is collected; meanwhile, the maximum absorbance corresponding to the ultraviolet absorption peaks of the signal probes with different particle sizes is measured, and the corresponding target content is calculated according to the change of the absorbance. According to the method, multiple targets can be detected simultaneously, the detection time is greatly shortened, and an ultraviolet spectrophotometer is simple and convenient to operate, high in sensitivity, good in stability and low in cost.

Description

technical field [0001] The invention belongs to the field of biochemical analysis, and relates to a multi-target simultaneous detection method based on the concentration change of latex microspheres. Background technique [0002] The rapid detection method has the advantages of simplicity, speed and accuracy, and has played an increasingly important role in safeguarding human health and protecting the ecological environment. According to different signal readout methods, rapid detection methods are mainly divided into optical methods, electrochemical methods and magnetic methods. Among them, the optical-based detection method is compatible with a variety of biochemical reactions, and is a non-contact detection mode, which has been widely used in clinical diagnosis, environmental monitoring, food safety and other fields. [0003] Among the optical-based rapid detection methods, the representative and widely used ones are the enzyme-linked immunoassay method, the colloidal go...

AI Technical Summary

Problems solved by technology

Fluorescence spectrum analysis can meet the needs of trace analysis, but few substances emit fluorescence by themselves or form a fluorescence measurement system. Although some analytes can be made to form fluorescence through chemical derivatization, it is difficult and greatly limits its application.

The immunoassay method based on fluorescent microspheres has also been applied to a certain extent, but the price of fluorescent microspheres is high, and it needs to be protected from light during use, which limits its further application

Although the above method has played an important role in the rapid detection of targets, its application is limited in the simultaneous detection of multiple targets in complex sample matrices, and the detection efficiency is low.

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