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Selenite-cystine enrichment broth and use thereof

A cystine enrichment solution and a cystine enrichment technology are applied in the field of microorganisms to achieve the effects of rapid bacterial growth, low cost, and favorable popularization and application.

Inactive Publication Date: 2015-05-27
刘汉斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 28g of this product, dissolve it in 1000ml of distilled water by heating, wait until it is cooled to 60°C, and pour it into a sterile plate. No autoclaving required.

[0023] Incubate at 36±1°C for 18-24 hours

[0024] Quality Control Strains growing situation Listeria monocytogenes - Staphylococcus aureus - Escherichia coli - / + salmonella (10-8)+++

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PUM

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Abstract

The invention relates to the field of microbes and especially relates to selenite-cystine enrichment broth. The selenite-cystine enrichment broth comprises 21g / L of GN enrichment broth, 4.0g / L of lactose, 5.0g / L of sodium hydrogen selenite, 7.55g / L of disodium hydrogen phosphate, 4.5g / L of L-cystine, 4.5g / L of sodium malonate and 2.9g / L of sodium dihydrogen phosphate. The selenite-cystine enrichment broth has a pH value of 7.0-7.2. Each liter of the GN enrichment broth comprises 20.0g of tryptone, 2.0g of glucose, 1.5g of dipotassium hydrogen phosphate, 3g of mannitol, 5.8g of sodium citrate, 0.33g of deoxysodium cholate, 5.0g of sodium chloride and 2.0g of dipotassium hydrogen phosphate. The selenite-cystine enrichment broth has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a selenite cystine enrichment liquid and an application thereof. Background technique [0002] The causative agent of salmonellosis. Enterobacteriaceae, Gram-negative enterobacteriaceae. Nearly a thousand species (or strains) have been discovered. According to its antigenic composition, Salmonella can be divided into basic bacterial groups such as A, B, C, D, and E. Among them, those related to human diseases mainly include A. paratyphi in Group A, B. paratyphi and Bacillus typhimurium in group B, C. paratyphi and Bacillus choleraesuis in group C, typhoid bacillus and Enteritidis bacteria in group D, etc. In addition to typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning. Salmo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/42
Inventor 刘汉斌
Owner 刘汉斌
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